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. 1988 Oct;54(10):2345–2348. doi: 10.1128/aem.54.10.2345-2348.1988

Rapid latex agglutination test for detection of staphylococcal enterotoxins A to E that uses high-density latex particles.

H Fujikawa 1, H Igarashi 1
PMCID: PMC204255  PMID: 3202627

Abstract

A rapid reversed passive latex agglutination method that uses high-density latex particles for the detection of staphylococcal enterotoxins (SE) A to E was developed. It took 3 h for incubation, much less than the 16 h needed with a customary latex agglutination test for SE detection such as a commercial test kit (SET-RPLA; Denka Seiken Co. Ltd., Tokyo, Japan). The rapid test was shown to be highly specific and sensitive for SE detection (detection limit, about 0.5 ng of SE per ml), comparable to the SET-RPLA test. The rapid test was also efficient in SE detection in foods and culture supernatants of staphylococcal strains, similar to the SET-RPLA test. This showed that a rapid test with high-density latex particles is fully reliable for use.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. HALL H. E., ANGELOTTI R., LEWIS K. H. DETECTION OF THE STAPHYLOCOCCAL ENTEROTOXINS IN FOOD. Health Lab Sci. 1965 Jul;2:179–191. [PubMed] [Google Scholar]
  2. Igarashi H., Fujikawa H., Shingaki M., Bergdoll M. S. Latex agglutination test for staphylococcal toxic shock syndrome toxin 1. J Clin Microbiol. 1986 Mar;23(3):509–512. doi: 10.1128/jcm.23.3.509-512.1986. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Lee A. C., Robbins R. N., Bergdoll M. S. Isolation of specific and common antibodies to staphylococcal enterotoxins A and E by affinity chromatography. Infect Immun. 1978 Aug;21(2):387–391. doi: 10.1128/iai.21.2.387-391.1978. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Morse S. A., Mah R. A. Microtiter hemagglutination-inhibition assay for staphylococcal enterotoxin B. Appl Microbiol. 1967 Jan;15(1):58–61. doi: 10.1128/am.15.1.58-61.1967. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Oda T. [Application of SP-sephadex chromatography to the purification of staphylococcal enterotoxin D and E (author's transl)]. Nihon Saikingaku Zasshi. 1980 May;35(3):559–567. [PubMed] [Google Scholar]
  6. Oda T. [Application of SP-sephadex chromatography to the purification of staphylococcal enterotoxins A, B and C2 (author's transl)]. Nihon Saikingaku Zasshi. 1978;33(6):743–752. [PubMed] [Google Scholar]
  7. Orth D. S. Statistical analysis and quality control in radioimmunoassays for staphylococcal enterotoxins A, B, and C. Appl Environ Microbiol. 1977 Dec;34(6):710–714. doi: 10.1128/aem.34.6.710-714.1977. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Park C. E., Szabo R. Evaluation of the reversed passive latex agglutination (RPLA) test kits for detection of staphylococcal enterotoxins A, B, C, and D in foods. Can J Microbiol. 1986 Sep;32(9):723–727. doi: 10.1139/m86-131. [DOI] [PubMed] [Google Scholar]
  9. Robbins R., Gould S., Bergdoll M. Detecting the enterotoxigenicity of Staphylococcus aureus strains. Appl Microbiol. 1974 Dec;28(6):946–950. doi: 10.1128/am.28.6.946-950.1974. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Salomon L. L., Tew R. W. Assay of staphylococcal enterotoxin B by latex agglutination. Proc Soc Exp Biol Med. 1968 Nov;129(2):539–542. doi: 10.3181/00379727-129-33364. [DOI] [PubMed] [Google Scholar]
  11. Saunders G. C., Bartlett M. L. Double-antibody solid-phase enzyme immunoassay for the detection of staphylococcal enterotoxin A. Appl Environ Microbiol. 1977 Nov;34(5):518–522. doi: 10.1128/aem.34.5.518-522.1977. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Todd J., Fishaut M., Kapral F., Welch T. Toxic-shock syndrome associated with phage-group-I Staphylococci. Lancet. 1978 Nov 25;2(8100):1116–1118. doi: 10.1016/s0140-6736(78)92274-2. [DOI] [PubMed] [Google Scholar]
  13. Yamada S., Igarashi H., Terayama T. Improved reversed passive hemagglutination for simple and rapid detection of staphycococcal enterotoxins A approximately E in food. Microbiol Immunol. 1977;21(12):675–682. doi: 10.1111/j.1348-0421.1977.tb00336.x. [DOI] [PubMed] [Google Scholar]

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