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. 1988 Oct;54(10):2536–2540. doi: 10.1128/aem.54.10.2536-2540.1988

Rapid hydrophobic grid membrane filter-enzyme-labeled antibody procedure for identification and enumeration of Escherichia coli O157 in foods.

E C Todd 1, R A Szabo 1, P Peterkin 1, A N Sharpe 1, L Parrington 1, D Bundle 1, M A Gidney 1, M B Perry 1
PMCID: PMC204308  PMID: 3060018

Abstract

An O-antigen-specific monoclonal antibody, labeled by horseradish peroxidase-protein A, was used in a hydrophobic grid membrane filter-enzyme-labeled antibody method for rapid detection of Escherichia coli O157 in foods. The method yielded presumptive identification within 24 h and recovered, on average, 95% of E. coli O157:H7 artificially inoculated into comminuted beef, veal, pork, chicken giblets, and chicken carcass washings. In food samples from two outbreaks involving E. coli O157:H7, the organism was isolated at levels of up to 10(3)/g. The lower limit of sensitivity was 10 E. coli O157 per g of meat. Specific typing for E. coli O157:H7 can be achieved through staining with labeled H7 antiserum or tube agglutination.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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