Skip to main content
PMC home page
Applied and Environmental Microbiology logoLink to Applied and Environmental Microbiology
. 1988 Nov;54(11):2871–2873. doi: 10.1128/aem.54.11.2871-2873.1988

Simplified X-ray film method for detection of bacterial volatilization of mercury chloride by Escherichia coli.

K Nakamura 1, H Nakahara 1
PMCID: PMC204390  PMID: 3063210

Abstract

A simplified X-ray film method for detecting bacterial production of Hgo by Escherichia coli was developed. There was excellent agreement of results between this X-ray film method and radioactive mercury volatilization assays. Although not quantitative, the X-ray film method has the advantages of being rapid, simple to perform, and inexpensive.

Full text

PDF
2873

Images in this article

2871Image
on p.2871
2872Image
on p.2872

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Clark D. L., Weiss A. A., Silver S. Mercury and organomercurial resistances determined by plasmids in Pseudomonas. J Bacteriol. 1977 Oct;132(1):186–196. doi: 10.1128/jb.132.1.186-196.1977. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Foster T. J., Nakahara H., Weiss A. A., Silver S. Transposon A-generated mutations in the mercuric resistance genes of plasmid R100-1. J Bacteriol. 1979 Oct;140(1):167–181. doi: 10.1128/jb.140.1.167-181.1979. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Kondo I., Ishikawa T., Nakahara H. Mercury and cadmium resistances mediated by the penicillinase plasmid in Staphylococcus aureus. J Bacteriol. 1974 Jan;117(1):1–7. doi: 10.1128/jb.117.1.1-7.1974. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Nakahara H., Ishikawa T., Sarai Y., Kondo I. Frequency of heavy-metal resistance in bacteria from inpatients in Japan. Nature. 1977 Mar 10;266(5598):165–167. doi: 10.1038/266165a0. [DOI] [PubMed] [Google Scholar]
  5. Nakahara H., Ishikawa T., Sarai Y., Kondo I., Kozukue H., Silver S. Linkage of mercury, cadmium, and arsenate and drug resistance in clinical isolates of Pseudomonas aeruginosa. Appl Environ Microbiol. 1977 Apr;33(4):975–976. doi: 10.1128/aem.33.4.975-976.1977. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Nakahara H., Kinscherf T. G., Silver S., Miki T., Easton A. M., Rownd R. H. Gene copy number effects in the mer operon of plasmid NR1. J Bacteriol. 1979 Apr;138(1):284–287. doi: 10.1128/jb.138.1.284-287.1979. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Nakahara H., Schottel J. L., Yamada T., Miyakawa Y., Asakawa M., Harville J., Silver S. Mercuric reductase enzymes from Streptomyces species and group B Streptococcus. J Gen Microbiol. 1985 May;131(5):1053–1059. doi: 10.1099/00221287-131-5-1053. [DOI] [PubMed] [Google Scholar]
  8. Nakahara H., Silver S., Miki T., Rownd R. H. Hypersensitivity to Hg2+ and hyperbinding activity associated with cloned fragments of the mercurial resistance operon of plasmid NR1. J Bacteriol. 1979 Oct;140(1):161–166. doi: 10.1128/jb.140.1.161-166.1979. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Nakamura K., Fujisaki T., Tamashiro H. Characteristics of Hg-resistant bacteria isolated from Minamata Bay sediment. Environ Res. 1986 Jun;40(1):58–67. doi: 10.1016/s0013-9351(86)80081-0. [DOI] [PubMed] [Google Scholar]
  10. Olson B. H., Barkay T., Colwell R. R. Role of plasmids in mercury transformation by bacteria isolated from the aquatic environment. Appl Environ Microbiol. 1979 Sep;38(3):478–485. doi: 10.1128/aem.38.3.478-485.1979. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Schottel J. L. The mercuric and organomercurial detoxifying enzymes from a plasmid-bearing strain of Escherichia coli. J Biol Chem. 1978 Jun 25;253(12):4341–4349. [PubMed] [Google Scholar]
  12. Schottel J., Mandal A., Clark D., Silver S., Hedges R. W. Volatilisation of mercury and organomercurials determined by inducible R-factor systems in enteric bacteria. Nature. 1974 Sep 27;251(5473):335–337. doi: 10.1038/251335a0. [DOI] [PubMed] [Google Scholar]
  13. Smith D. H. R factors mediate resistance to mercury, nickel, and cobalt. Science. 1967 May 26;156(3778):1114–1116. doi: 10.1126/science.156.3778.1114. [DOI] [PubMed] [Google Scholar]
  14. Summers A. O., Silver S. Mercury resistance in a plasmid-bearing strain of Escherichia coli. J Bacteriol. 1972 Dec;112(3):1228–1236. doi: 10.1128/jb.112.3.1228-1236.1972. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Summers A. O., Silver S. Microbial transformations of metals. Annu Rev Microbiol. 1978;32:637–672. doi: 10.1146/annurev.mi.32.100178.003225. [DOI] [PubMed] [Google Scholar]
  16. Timoney J. F., Port J., Giles J., Spanier J. Heavy-metal and antibiotic resistance in the bacterial flora of sediments of New York Bight. Appl Environ Microbiol. 1978 Sep;36(3):465–472. doi: 10.1128/aem.36.3.465-472.1978. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Tonomura K., Maeda K., Futai F., Nakagami T., Yamada M. Stimulative vaporization of phenylmercuric acetate by mercury-resistant bacteria. Nature. 1968 Feb 17;217(5129):644–646. doi: 10.1038/217644b0. [DOI] [PubMed] [Google Scholar]

Articles from Applied and Environmental Microbiology are provided here courtesy of American Society for Microbiology (ASM)

RESOURCES