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. 1980 Dec;70(4):537–544. doi: 10.1111/j.1476-5381.1980.tb09772.x

Effects of vasodilator drugs, alkaline phosphatase, and cyclic AMP-dependent protein kinase on the 45calcium uptake of sarcolemmal microsomes from human umbilical arteries.

V A Kreye, E Schlicker
PMCID: PMC2044383  PMID: 6258679

Abstract

1 A microsomal fraction was prepared from human umbilical arteries by differential centrifugation. The preparation was capable of an oxalate-stimulated Ca2+ uptake at a mean rate of 0.74 nmol Ca2+ mg-1 protein min-1 which could be inhibited by a Ca2+ ionophore, A 23 187, and by Tween 80. 2 The rate of Ca2+ uptake in the fraction obtained by density gradient fractionation paralleled 5'-nucleotidase activity suggesting that vesicles of predominantly sarcolemmal origin were responsible for the microsomal Ca2+ uptake. 3 Cyclic adenosine 3',5'-monophosphate-dependent protein kinase enhanced membrane phosphorylation but did not affect Ca2+ uptake. Preincubation with alkaline phosphatase reduced membrane phosphorylation to a greater extent than Ca2+ uptake. These data are not in favour of a close correlation between Ca2+ uptake and phosphorylation. 4 None of 15 vasodilator drugs (bencyclane, carbocromen, diazoxide, dilazep, hydralazine, indapamide, isosorbide dinitrate, methyl-isobutyl-xanthine, minoxidil, naftidrofuryl, nitroglycerine, prenylamine, sodium nitroprusside, tetracaine, and verapamil) had any effect on Ca2+ uptake at 10(-5) M. This suggests that vasodilator drugs do not act by a direct influence on the Ca2+ pumps of vascular smooth muscle cells.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Allen J. C. Ca++-binding properties of cznine aortic microsomes: lack of effect of c-AMP. Blood Vessels. 1977 Mar;14(2):91–104. doi: 10.1159/000158117. [DOI] [PubMed] [Google Scholar]
  2. Clyman R. I., Manganiello V. C., Lovell-Smith C. J., Vaughan M. Calcium uptake by subcellular fractions of human umbilical artery. Am J Physiol. 1976 Oct;231(4):1074–1081. doi: 10.1152/ajplegacy.1976.231.4.1074. [DOI] [PubMed] [Google Scholar]
  3. Klinner U., Ehlers D., Fermum R., Meisel P. Versuche zum Wirkungsmechanismus von Gefässspasmolytika. 4. Wirkung von Nitrosprussid-Natrium, Nitroglyzerin, Prenylamin und Verapamil auf die Ca-Aufnahme von Mikrosomen aus glatter Gefässmuskulatur. Acta Biol Med Ger. 1977;36(7-8):1143–1148. [PubMed] [Google Scholar]
  4. Kreye V. A., Baron G. D., Lüth J. B., Schmidt-Gayk H. Mode of action of sodium nitroprusside on vascular smooth muscle. Naunyn Schmiedebergs Arch Pharmacol. 1975;288(4):381–402. doi: 10.1007/BF00501284. [DOI] [PubMed] [Google Scholar]
  5. Kwan C. Y., Belbeck L., Daniel E. E. Abnormal biochemistry of vascular smooth muscle plasma membrane as an important factor in the initiation and maintenance of hypertension in rats. Blood Vessels. 1979;16(5):259–268. doi: 10.1159/000158214. [DOI] [PubMed] [Google Scholar]
  6. LOWRY O. H., ROSEBROUGH N. J., FARR A. L., RANDALL R. J. Protein measurement with the Folin phenol reagent. J Biol Chem. 1951 Nov;193(1):265–275. [PubMed] [Google Scholar]
  7. Nishikori K., Takenaka T., Maeno H. Stimulation of microsomal calcium uptake and protein phosphorylation by adenosine cyclic 3', 5'-monophosphate in rat uterus. Mol Pharmacol. 1977 Jul;13(4):671–678. [PubMed] [Google Scholar]
  8. Reuter H., Blaustein M. P., Haeusler G. Na-Ca exchange and tension development in arterial smooth muscle. Philos Trans R Soc Lond B Biol Sci. 1973 Mar 15;265(867):87–94. doi: 10.1098/rstb.1973.0011. [DOI] [PubMed] [Google Scholar]
  9. Thorens S., Haeusler G. Effects of adenosine 3' : 5'-monophosphate and guanosine 3' : 5'-monophosphate on calcium uptake and phosphorylation in membrane fractions of vascular smooth muscle. Biochim Biophys Acta. 1978 Sep 22;512(2):415–428. doi: 10.1016/0005-2736(78)90264-x. [DOI] [PubMed] [Google Scholar]
  10. Thorens S., Haeusler G. Effects of some vasodilators on calcium translocation in intact and fractionated vascular smooth muscle. Eur J Pharmacol. 1979 Feb 15;54(1-2):79–91. doi: 10.1016/0014-2999(79)90410-2. [DOI] [PubMed] [Google Scholar]
  11. Wei J. W., Janis R. A., Daniel E. E. Isolation and characterization of plasma membrane from rat mesenteric arteries. Blood Vessels. 1976;13(5):279–292. doi: 10.1159/000158098. [DOI] [PubMed] [Google Scholar]
  12. Wuytack F., Landon E., Fleischer S., Hardman J. G. The calcium accumulation in a microsomal fraction from porcine coronary artery smooth muscle. A study of the heterogeneity of the fraction. Biochim Biophys Acta. 1978 May 3;540(2):253–269. doi: 10.1016/0304-4165(78)90138-1. [DOI] [PubMed] [Google Scholar]

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