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. 1980;71(2):435–444. doi: 10.1111/j.1476-5381.1980.tb10956.x

Adrenaline activation of prejunctional beta-adrenoceptors in guinea-pig atria.

H Majewski, M W McCulloch, M J Rand, D F Story
PMCID: PMC2044449  PMID: 6258697

Abstract

1. Adrenaline in a concentration of 1.0 microM depressed the stimulation-induced efflux of tritium from the guinea-pig atria incubated with [3H]-noradrenaline, whereas adrenaline in a concentration of 0.5 nM significantly enhanced the stimulation-induced efflux of tritium. This enhancement was blocked by metoprolol (0.1 microM) and thus appears to be mediated by beta-adrenoceptors. 2. In guinea-pig atria incubated with unlabelled adrenaline and then with [3H]-noradrenaline, both catecholamines were released by field stimulation. In such atria metoprolol, practolol, oxprenolol or propranolol decreased the stimulation-induced efflux of tritium. These effects did not occur if the atria were incubated with unlabelled noradrenaline and then with [3H]-noradrenaline, suggesting that neuronally released adrenaline activates prejunctional beta-adrenoceptors. 3. The effect of oxprenolol in decreasing the release of tritium from guinea-pig atria, incubated with unlabelled adrenaline and then with [3H]-noradrenaline was greater in the presence of phentolamine. This may reflect the alpha-adrenoceptor blocking activity of oxprenolol.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Adler-Graschinsky E., Langer S. Z. Possible role of a beta-adrenoceptor in the regulation of noradrenaline release by nerve stimulation through a positive feed-back mechanism. Br J Pharmacol. 1975 Jan;53(1):43–50. doi: 10.1111/j.1476-5381.1975.tb07328.x. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Dahlöf C., Ljung B., Ablad B. Increased noradrenaline release in rat portal vein during sympathetic nerve stimulation due to activation of presynaptic beta-adrenoceptors by noradrenaline and adrenaline. Eur J Pharmacol. 1978 Jul 1;50(1):75–78. doi: 10.1016/0014-2999(78)90255-8. [DOI] [PubMed] [Google Scholar]
  3. De Champlain J., Cousineau D., Van Amerigen M. R., Marc-Aurèle J., Yamaguchi N. The role of the sympathetic system in experimental and human hypertension. Postgrad Med J. 1977;53 (Suppl 3):15–30. [PubMed] [Google Scholar]
  4. Franco-Morselli R., Elghozi J. L., Joly E., Di Giuilio S., Meyer P. Increased plasma adrenaline concentrations in benign essential hypertension. Br Med J. 1977 Nov 12;2(6097):1251–1254. doi: 10.1136/bmj.2.6097.1251. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Hedqvist P., Moawad A. Presynaptic alpha- and beta-adrenoceptor medicated control of noradrenaline release in human oviduct. Acta Physiol Scand. 1975 Dec;95(4):494–496. doi: 10.1111/j.1748-1716.1975.tb10079.x. [DOI] [PubMed] [Google Scholar]
  6. Lands A. M., Arnold A., McAuliff J. P., Luduena F. P., Brown T. G., Jr Differentiation of receptor systems activated by sympathomimetic amines. Nature. 1967 May 6;214(5088):597–598. doi: 10.1038/214597a0. [DOI] [PubMed] [Google Scholar]
  7. Langer S. Z. The metabolism of (3H)noradrenaline released by electrical stimulation from the isolated nictitating membrane of the cat and from the vas deferens of the rat. J Physiol. 1970 Jul;208(3):515–546. doi: 10.1113/jphysiol.1970.sp009135. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Majewski H., Story D. F. Paper chromatographic separation of noradrenaline and its major metabolites. J Chromatogr. 1977 Sep 11;139(1):218–222. doi: 10.1016/s0021-9673(01)84149-2. [DOI] [PubMed] [Google Scholar]
  9. Rand M., Law M., Story D. F., McCulloch M. W. Effects of beta-adrenoreceptor blocking drugs on adrenergic transmission. Drugs. 1976;11(Suppl 1):134–143. doi: 10.2165/00003495-197600111-00029. [DOI] [PubMed] [Google Scholar]
  10. Stj-5aarne L., Brundin J. Dual adreoceptor-mediated control of noradrenaline secretion from human vasoconstrictor nerves: facilitation by BETA-receptors and inhibitor by alpha-receptors. Acta Physiol Scand. 1975 May;94(1):139–141. doi: 10.1111/j.1748-1716.1975.tb05872.x. [DOI] [PubMed] [Google Scholar]
  11. Stjärne L., Brundin J. Beta2-adrenoceptors facilitating noradrenaline secretion from human vasoconstrictor nerves. Acta Physiol Scand. 1976 Mar;97(1):88–93. doi: 10.1111/j.1748-1716.1976.tb10238.x. [DOI] [PubMed] [Google Scholar]
  12. Westfall T. C., Peach M. J., Tittermary V. Enhancement of the electrically induced release of norepinephrine from the rat portal vein: mediation by beta 2-adrenoceptors. Eur J Pharmacol. 1979 Sep 1;58(1):67–74. doi: 10.1016/0014-2999(79)90341-8. [DOI] [PubMed] [Google Scholar]
  13. Yamaguchi N., de Champlain J., Nadeau R. A. Regulation of norepinephrine release from cardiac sympathetic fibers in the dog by presynaptic alpha- and beta-receptors. Circ Res. 1977 Jul;41(1):108–117. doi: 10.1161/01.res.41.1.108. [DOI] [PubMed] [Google Scholar]
  14. de Champlain J., Farley L., Cousineau D., van Ameringen M. R. Circulating catecholamine levels in human and experimental hypertension. Circ Res. 1976 Feb;38(2):109–114. doi: 10.1161/01.res.38.2.109. [DOI] [PubMed] [Google Scholar]

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