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. 1983 Feb;78(2):395–404. doi: 10.1111/j.1476-5381.1983.tb09404.x

Interactions of dopamine and the release of [3H]-taurine and [3H]-glycine from the isolated retina of the rat

CJ Pycock, LFP Smith
PMCID: PMC2044701  PMID: 6187404

Abstract

1 The dose-related, calcium-dependent, potassium-stimulated release of preloaded [3H]-dopamine from the superfused rat retina has been demonstrated.

2 A high-affinity uptake system for dopamine exists in rat retina in vitro; Km value was calculated as 1.89 μM, Vmax value as 1.4 nmol g-1 tissue h-1.

3 Dopamine (0.8 and 4 mM) inhibited the spontaneous release of [3H]-glycine from retina, and in the case of 0.8 mM dopamine this inhibitory effect was antagonized by 10 μM (+)-butaclamol but not by 10 μM (-)-butaclamol.

4 The potassium-evoked (25 mM) release of [3H]-glycine from rat retina was similarly inhibited by dopamine (0.4-4 mM) in a dose-related manner when added to the superfusate with the potassium. The effect of 0.8 mM dopamine was antagonized by 10 μM (+)-butaclamol but not by 10 μM (-)-butaclamol.

5 Dopamine (4 mM) significantly reduced the spontaneous release of [3H]-taurine from rat retina.

6 The potassium-stimulated (25 mM) release of [3H]-taurine occurred after the cessation of the depolarizing stimulus. This delayed release of [3H]-taurine was unaffected if dopamine was applied to the superfusate at the same time as the potassium, but it was significantly reduced if dopamine (0.8 and 4 mM) was applied after the depolarizing stimulus had been removed and during the actual amino acid release phase.

7 The inhibition of K+-stimulated (25 mM) delayed release of [3H]-taurine by applying dopamine (0.8 mM) after the depolarizing stimulus was blocked by 10 μM (+)-butaclamol but not by 10 μM (-)-butaclamol.

8 The results are discussed with respect to the possible neurotransmitter role for dopamine within the rat retina, and its possible interaction with glycine and taurine.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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