Skip to main content
PMC home page
British Journal of Pharmacology logoLink to British Journal of Pharmacology
. 1983 Jun;79(2):363–371. doi: 10.1111/j.1476-5381.1983.tb11008.x

The pharmacological modulation of [3H]-disaturated phosphatidylcholine overflow from perifused lung slices of adult rats: a new method for the study of lung surfactant secretion.

A M Gilfillan, M Hollingsworth, A W Jones
PMCID: PMC2044872  PMID: 6689133

Abstract

Lung slices from adult rats incubated in [methyl-3H]-choline chloride formed [3H]-disaturated phosphatidylcholine ( [3H]-DSPC) which was used as an index of lung surfactant. The slices were perifused after 3 h incubation in [methyl-3H]-choline chloride and the overflow of [3H]-DSPC, as a rate coefficient, was used as a measure of surfactant secretion. The basal overflow of [3H]-DSPC rapidly declined over the first 30 min of perifusion and then declined slowly. Salbutamol induced a prolonged, and sometimes delayed, increase in [3H]-DSPC overflow, which was reduced by (+/-)-propranolol. Potassium chloride produced an immediate, and usually transient, increase in [3H]-DSPC overflow which was not modified by atropine or (+/-)-propranolol. Adenosine 5'-triphosphate, but not phenylephrine, also increased [3H]-DSPC overflow. This method can measure the magnitude and time-course of lung surfactant secretion induced by drugs.

Full text

PDF
367

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Abdellatif M. M., Hollingsworth M. Effect of oxotremorine and epinephrine on lung surfactant secretion in neonatal rabbits. Pediatr Res. 1980 Aug;14(8):916–920. doi: 10.1203/00006450-198008000-00004. [DOI] [PubMed] [Google Scholar]
  2. Ahmed A., Chiswick M. L. Origin of osmophilic inclusion bodies in type II pneumocytes. J Pathol. 1974 Jul;113(3):161–163. doi: 10.1002/path.1711130305. [DOI] [PubMed] [Google Scholar]
  3. Brown L. A., Longmore W. J. Adrenergic and cholinergic regulation of lung surfactant secretion in the isolated perfused rat lung and in the alveolar type II cell in culture. J Biol Chem. 1981 Jan 10;256(1):66–72. [PubMed] [Google Scholar]
  4. Case R. M. Synthesis, intracellular transport and discharge of exportable proteins in the pancreatic acinar cell and other cells. Biol Rev Camb Philos Soc. 1978 May;53(2):211–354. doi: 10.1111/j.1469-185x.1978.tb01437.x. [DOI] [PubMed] [Google Scholar]
  5. Chevalier G., Collet A. J. In vivo incorporation of choline- 3 H, leucine- 3 H and galactose- 3 H in alveolar type II pneumocytes in relation to surfactant synthesis. A quantitative radoautographic study in mouse by electron microscopy. Anat Rec. 1972 Nov;174(3):289–310. doi: 10.1002/ar.1091740303. [DOI] [PubMed] [Google Scholar]
  6. Delahunty T. J., Johnston J. M. Neurohumoral control of pulmonary surfactant secretion. Lung. 1979;157(1):45–51. doi: 10.1007/BF02713593. [DOI] [PubMed] [Google Scholar]
  7. Dobbs L. G., Mason R. J. Pulmonary alveolar type II cells isolated from rats. Release of phosphatidylcholine in response to beta-adrenergic stimulation. J Clin Invest. 1979 Mar;63(3):378–387. doi: 10.1172/JCI109313. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Epstein M. F., Farrell P. M. The choline incorporation pathway: primary mechanism for de novo lecithin synthesis in fetal primate lung. Pediatr Res. 1975 Aug;9(8):658–665. doi: 10.1203/00006450-197508000-00009. [DOI] [PubMed] [Google Scholar]
  9. Gilfillan A. M., Harkes A., Hollingsworth M. Secretion of lung surfactant following delivery after uterine section. J Dev Physiol. 1980 Feb-Apr;2(1-2):101–110. [PubMed] [Google Scholar]
  10. Goerke J. Lung surfactant. Biochim Biophys Acta. 1974 Dec 16;344(3-4):241–261. doi: 10.1016/0304-4157(74)90009-4. [DOI] [PubMed] [Google Scholar]
  11. Hollingsworth M. Mechanical responses of rat isolated uterine horns to transmural stimulation. Br J Pharmacol. 1975 Sep;55(1):41–46. doi: 10.1111/j.1476-5381.1975.tb07607.x. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Jack D. An introduction to salbutamol and other modern beta-adrenoreceptor stimulants. Postgrad Med J. 1971 Mar;47(Suppl):8–11. [PubMed] [Google Scholar]
  13. Lacy P. E. Endocrine secretory mechanisms. A review. Am J Pathol. 1975 Apr;79(1):170–188. [PMC free article] [PubMed] [Google Scholar]
  14. Marino P. A., Rooney S. A. Surfactant secretion in a newborn rabbit lung slice model. Biochim Biophys Acta. 1980 Dec 5;620(3):509–519. doi: 10.1016/0005-2760(80)90143-5. [DOI] [PubMed] [Google Scholar]
  15. Marino P. A., Rooney S. A. The effect of labor on surfactant secretion in newborn rabbit lung slices. Biochim Biophys Acta. 1981 May 22;664(2):389–396. doi: 10.1016/0005-2760(81)90061-8. [DOI] [PubMed] [Google Scholar]
  16. Mason R. J., Nellenbogen J., Clements J. A. Isolation of disaturated phosphatidylcholine with osmium tetroxide. J Lipid Res. 1976 May;17(3):281–284. [PubMed] [Google Scholar]
  17. Oyarzún M. J., Clements J. A. Ventilatory and cholinergic control of pulmonary surfactant in the rabbit. J Appl Physiol Respir Environ Exerc Physiol. 1977 Jul;43(1):39–45. doi: 10.1152/jappl.1977.43.1.39. [DOI] [PubMed] [Google Scholar]
  18. Smith B. T., Bogues W. G. Effects of drugs and hormones on lung maturation in experimental animal and man. Pharmacol Ther. 1980;9(1):51–74. doi: 10.1016/0163-7258(80)90016-9. [DOI] [PubMed] [Google Scholar]
  19. Van Golde L. M. Metabolism of phospholipids in the lung. Am Rev Respir Dis. 1976 Nov;114(5):977–1000. doi: 10.1164/arrd.1976.114.5.977. [DOI] [PubMed] [Google Scholar]
  20. WROBLEWSKI F., LADUE J. S. Lactic dehydrogenase activity in blood. Proc Soc Exp Biol Med. 1955 Oct;90(1):210–213. doi: 10.3181/00379727-90-21985. [DOI] [PubMed] [Google Scholar]
  21. Williams J. A. Electrical correlates of secretion in endocrine and exocrine cells. Fed Proc. 1981 Feb;40(2):128–134. [PubMed] [Google Scholar]

Articles from British Journal of Pharmacology are provided here courtesy of The British Pharmacological Society

RESOURCES