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. 1983 Dec;80(4):703–710. doi: 10.1111/j.1476-5381.1983.tb10061.x

Studies on an isolated innervated preparation of guinea-pig trachea.

J G Blackman, D J McCaig
PMCID: PMC2045041  PMID: 6571227

Abstract

An isolated preparation of the guinea-pig trachea with intact parasympathetic and sympathetic innervation has been devised. Responses to nerve stimulation were recorded as increases or decreases in intraluminal pressure from the fluid-filled trachea. The preparation maintained a positive resting intraluminal pressure of 3-4 cmH2O. This was unaffected by atropine, hexamethonium or propranolol. Brief pressor responses, which could be completely blocked by atropine or hexamethonium, were obtained by applying short trains of stimuli to the cervical segment of the right vagus. The amplitude of responses was frequency-dependent up to a maximum at 40 Hz. Depressor responses, more delayed and prolonged than the pressor responses and blocked by propranolol but not by hexamethonium, were obtained by stimulation of the right cervical sympathetic trunk or stellate ganglion in 70% of preparations. Dual pressor-depressor responses were observed in the remaining 30% of preparations. The pressor component was blocked by atropine, the depressor component by propranolol. In the presence of atropine and propranolol, sustained sympathetic stimulation sometimes evoked a small, delayed pressor response which was blocked by phentolamine. Under the same conditions, transmural stimulation produced a depressor response evidently due to non-adrenergic, non-cholinergic nerves. Spontaneous activity was observed in some preparations under normal conditions, but could always be evoked by hypoxia. Responses to sympathetic stimulation were reduced both by hypoxia and during periods of spontaneous activity.(ABSTRACT TRUNCATED AT 250 WORDS)

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Selected References

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  1. Beinfield W. H., Seifter J. Spontaneous mechanical activities of dog trachealis muscle in vivo. J Appl Physiol Respir Environ Exerc Physiol. 1980 Feb;48(2):320–328. doi: 10.1152/jappl.1980.48.2.320. [DOI] [PubMed] [Google Scholar]
  2. Bose R., Bose D. Excitation-contraction coupling in multiunit tracheal smooth muscle during metabolic depletion: induction of rhythmicity. Am J Physiol. 1977 Jul;233(1):C8–13. doi: 10.1152/ajpcell.1977.233.1.C8. [DOI] [PubMed] [Google Scholar]
  3. Cabezas G. A., Graf P. D., Nadel J. A. Sympathetic versus parasympathetic nervous regulation of airways in dogs. J Appl Physiol. 1971 Nov;31(5):651–655. doi: 10.1152/jappl.1971.31.5.651. [DOI] [PubMed] [Google Scholar]
  4. Chesrown S. E., Venugopalan C. S., Gold W. M., Drazen J. M. In vivo demonstration of nonadrenergic inhibitory innervation of the guinea pig trachea. J Clin Invest. 1980 Feb;65(2):314–320. doi: 10.1172/JCI109674. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Coburn R. F., Tomita T. Evidence for nonadrenergic inhibitory nerves in the guinea pig trachealis muscle. Am J Physiol. 1973 May;224(5):1072–1080. doi: 10.1152/ajplegacy.1973.224.5.1072. [DOI] [PubMed] [Google Scholar]
  6. Coleman R. A., Farmer J. B. The inducement of tone and its inhibition in isolated tracheal muscle. J Pharm Pharmacol. 1971 Mar;23(3):220–222. doi: 10.1111/j.2042-7158.1971.tb08648.x. [DOI] [PubMed] [Google Scholar]
  7. Diamond L., O'Donnell M. A nonadrenergic vagal inhibitory pathway to feline airways. Science. 1980 Apr 11;208(4440):185–188. doi: 10.1126/science.7361114. [DOI] [PubMed] [Google Scholar]
  8. Doidge J. M., Satchell D. G. Adrenergic and non-adrenergic inhibitory nerves in mammalian airways. J Auton Nerv Syst. 1982 Mar;5(2):83–99. doi: 10.1016/0165-1838(82)90030-3. [DOI] [PubMed] [Google Scholar]
  9. Everitt B. J., Cairncross K. D. Adrenergic receptors in the guinea-pig trachea. J Pharm Pharmacol. 1969 Feb;21(2):97–102. doi: 10.1111/j.2042-7158.1969.tb08204.x. [DOI] [PubMed] [Google Scholar]
  10. Fleisch J. H., Maling H. M., Brodie B. B. Evidence for existence of alpha-adrenergic receptors in the mammalian trachea. Am J Physiol. 1970 Feb;218(2):596–599. doi: 10.1152/ajplegacy.1970.218.2.596. [DOI] [PubMed] [Google Scholar]
  11. Green M., Widdicombe J. G. The effects of ventilation of dogs with different gas mixtures on airway calibre and lung mechanics. J Physiol. 1966 Oct;186(2):363–381. doi: 10.1113/jphysiol.1966.sp008040. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Hebb C. O. Bronchomotor responses to stimulation of the stellate ganglia and to injection of acetylcholine in isolated perfused guinea-pig lungs. J Physiol. 1940 Dec 20;99(1):57–75. doi: 10.1113/jphysiol.1940.sp003878. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Irvin C. G., Boileau R., Tremblay J., Martin R. R., Macklem P. T. Bronchodilatation: noncholinergic, nonadrenergic mediation demonstrated in vivo in the cat. Science. 1980 Feb 15;207(4432):791–792. doi: 10.1126/science.7352292. [DOI] [PubMed] [Google Scholar]
  14. Ito Y., Takeda K. Non-adrenergic inhibitory nerves and putative transmitters in the smooth muscle of cat trachea. J Physiol. 1982 Sep;330:497–511. doi: 10.1113/jphysiol.1982.sp014355. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Richardson J., Béland J. Nonadrenergic inhibitory nervous system in human airways. J Appl Physiol. 1976 Nov;41(5 Pt 1):764–771. doi: 10.1152/jappl.1976.41.5.764. [DOI] [PubMed] [Google Scholar]
  16. Russell J. A. Innervation of airway smooth muscle in the dog. Bull Eur Physiopathol Respir. 1980 Sep-Oct;16(5):671–692. [PubMed] [Google Scholar]
  17. Souhrada J. F., Dickey D. W. Effect of antigen challenge on sensitized guinea pig trachea. Respir Physiol. 1976 Aug;27(2):241–251. doi: 10.1016/0034-5687(76)90077-3. [DOI] [PubMed] [Google Scholar]
  18. Souhrada J. F., Dickey D. W. Mechanical activities of trachea as measured in vitro and in vivo. Respir Physiol. 1976 Feb;26(1):27–40. doi: 10.1016/0034-5687(76)90049-9. [DOI] [PubMed] [Google Scholar]
  19. Souhrada J. F., Kivity S. The effect of some factors on the inhibitory nervous systems of airway smooth muscle. Respir Physiol. 1982 Jun;48(3):297–308. doi: 10.1016/0034-5687(82)90035-4. [DOI] [PubMed] [Google Scholar]
  20. Stephens N. L., Kroeger E. Effect of hypoxia on airway smooth muscle mechanics and electrophysiology. J Appl Physiol. 1970 May;28(5):630–635. doi: 10.1152/jappl.1970.28.5.630. [DOI] [PubMed] [Google Scholar]
  21. Suzuki H., Morita K., Kuriyama H. Innervation and properties of the smooth muscle of the dog trachea. Jpn J Physiol. 1976;26(3):303–320. doi: 10.2170/jjphysiol.26.303. [DOI] [PubMed] [Google Scholar]
  22. WIDDICOMBE J. G. Regulation of tracheobronchial smooth muscle. Physiol Rev. 1963 Jan;43:1–37. doi: 10.1152/physrev.1963.43.1.1. [DOI] [PubMed] [Google Scholar]
  23. Wellens D. Pharmacological reactivity of an isolated tracheal preparation of the guinea-pig. Med Pharmacol Exp Int J Exp Med. 1966;14(5):427–434. doi: 10.1159/000135817. [DOI] [PubMed] [Google Scholar]
  24. Widdicombe J. G. Action potentials in parasympathetic and sympathetic efferent fibres to the trachea and lungs of dogs and cats. J Physiol. 1966 Sep;186(1):56–88. doi: 10.1113/jphysiol.1966.sp008020. [DOI] [PMC free article] [PubMed] [Google Scholar]
  25. Yip P., Palombini B., Coburn R. F. Inhibitory innervation to the guinea pig trachealis muscle. J Appl Physiol Respir Environ Exerc Physiol. 1981 Feb;50(2):374–382. doi: 10.1152/jappl.1981.50.2.374. [DOI] [PubMed] [Google Scholar]
  26. Zorychta E., Richardson J. B. Control of smooth muscle in human airways. Bull Eur Physiopathol Respir. 1980 Sep-Oct;16(5):581–586. [PubMed] [Google Scholar]

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