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. 1993 May;175(10):3121–3130. doi: 10.1128/jb.175.10.3121-3130.1993

Synthesis of peptidoglycan and membrane during the division cycle of rod-shaped, gram-negative bacteria.

D Gally 1, K Bray 1, S Cooper 1
PMCID: PMC204634  PMID: 8491728

Abstract

A modified procedure for determining the pattern of peptidoglycan synthesis during the division cycle has allowed the measurement of the rate of side wall synthesis during the division cycle without the contribution due to pole formation. As predicted by a model proposing that the surface growth of the cell is regulated by mass increase, we find a decrease in side wall synthesis in the latter half of the division cycle. This supports the proposal that, upon invagination, pole growth accommodates a significant proportion of the increasing cell mass and that residual side wall growth occurs in response to the residual mass increase not accommodated by pole volume. The observed side wall synthesis patterns support the proposal that mass increase is a major, and possibly sole, regulator of bacterial surface increase. Membrane synthesis during the division cycle of the gram-negative, rod-shaped bacteria Escherichia coli and Salmonella typhimurium has also been measured with similar methods. The rate of membrane synthesis--measured by incorporation of radioactive glycerol or palmitate relative to simultaneous labeling with radioactive leucine--exhibits the same pattern as peptidoglycan synthesis. The results are compatible with a model of cell surface growth containing the following elements. (i) During the period of the division cycle prior to invagination, growth of the cell occurs predominantly in the side wall and the cell grows only in length. (ii) When invagination begins, pole growth accommodates some cytoplasmic increase, leading to a concomitant decrease in side wall synthesis. (iii) Surface synthesis increases relative to mass synthesis during the last part of the division cycle because of pole formation. It is proposed here that membrane synthesis passively follows the pattern of peptidoglycan synthesis during the division cycle.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. BLIGH E. G., DYER W. J. A rapid method of total lipid extraction and purification. Can J Biochem Physiol. 1959 Aug;37(8):911–917. doi: 10.1139/o59-099. [DOI] [PubMed] [Google Scholar]
  2. Cooper S., Hsieh M. L. The rate and topography of cell wall synthesis during the division cycle of Escherichia coli using N-acetylglucosamine as a peptidoglycan label. J Gen Microbiol. 1988 Jun;134(6):1717–1721. doi: 10.1099/00221287-134-6-1717. [DOI] [PubMed] [Google Scholar]
  3. Cooper S. Leucine uptake and protein synthesis are exponential during the division cycle of Escherichia coli B/r. J Bacteriol. 1988 Jan;170(1):436–438. doi: 10.1128/jb.170.1.436-438.1988. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Cooper S. Rate and topography of cell wall synthesis during the division cycle of Salmonella typhimurium. J Bacteriol. 1988 Jan;170(1):422–430. doi: 10.1128/jb.170.1.422-430.1988. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Joseleau-Petit D., Kepes F., Peutat L., D'Ari R., Rothfield L. I. Biosynthesis of a membrane adhesion zone fraction throughout the cell cycle of Escherichia coli. J Bacteriol. 1990 Nov;172(11):6573–6575. doi: 10.1128/jb.172.11.6573-6575.1990. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Joseleau-Petit D., Képès F., Peutat L., D'Ari R., Képès A. DNA replication initiation, doubling of rate of phospholipid synthesis, and cell division in Escherichia coli. J Bacteriol. 1987 Aug;169(8):3701–3706. doi: 10.1128/jb.169.8.3701-3706.1987. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Koch A. L. The surface stress theory of microbial morphogenesis. Adv Microb Physiol. 1983;24:301–366. doi: 10.1016/s0065-2911(08)60388-4. [DOI] [PubMed] [Google Scholar]
  8. Koch A. L., Woeste S. Elasticity of the sacculus of Escherichia coli. J Bacteriol. 1992 Jul;174(14):4811–4819. doi: 10.1128/jb.174.14.4811-4819.1992. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Kubitschek H. E. Buoyant density variation during the cell cycle in microorganisms. Crit Rev Microbiol. 1987;14(1):73–97. doi: 10.3109/10408418709104436. [DOI] [PubMed] [Google Scholar]
  10. Pierucci O., Melzer M., Querini C., Rickert M., Krajewski C. Comparison among patterns of macromolecular synthesis in Escherichia coli B/r at growth rates of less and more than one doubling per hour at 37 degrees C. J Bacteriol. 1981 Nov;148(2):684–696. doi: 10.1128/jb.148.2.684-696.1981. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Pierucci O. Phospholipid synthesis during the cell division cycle of Escherichia coli. J Bacteriol. 1979 May;138(2):453–460. doi: 10.1128/jb.138.2.453-460.1979. [DOI] [PMC free article] [PubMed] [Google Scholar]

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