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. 1971 Aug;64(2):295–304.

Target Cell Destruction In Vitro by Concanavalin A-Stimulated Lymphoid Cells

Howard J Schwartz, Frank Wilson
PMCID: PMC2047574  PMID: 5142268

Abstract

Guinea pig lymphoid cells obtained from lymph nodes and spleens of nonimmune animals are cytotoxic for mouse L-cell fibroblasts on incubation with concanavalin A. This effect is blocked by methyl-α-D-mannoside. The results indicate that this effect may be due to the release of a lymphotoxin by the concanavalin A-stimulated lymphocytes.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Granger G. A., Williams T. W. Lymphocyte cytotoxicity in vitro: activation and release of a cytotoxic factor. Nature. 1968 Jun 29;218(5148):1253–1254. doi: 10.1038/2181253a0. [DOI] [PubMed] [Google Scholar]
  2. HARRIS H., ROBSON E. B. Precipitin reactions between extracts of seeds of Canavalia ensiformis (Jack Bean) and normal and pathological serum proteins. Vox Sang. 1963 May-Jun;8:348–355. [PubMed] [Google Scholar]
  3. HOLM G., PERLMANN P., WERNER B. PHYTOHAEMAGGLUTININ-INDUCED CYTOTOXIC ACTION OF NORMAL LYMPHOID CELLS ON CELLS IN TISSUE CULTURE. Nature. 1964 Aug 22;203:841–843. doi: 10.1038/203841a0. [DOI] [PubMed] [Google Scholar]
  4. Leon M. A. Concanavalin A reaction with human normal immunoglobulin G and myeloma immunoglobulin G. Science. 1967 Dec 8;158(3806):1325–1326. doi: 10.1126/science.158.3806.1325. [DOI] [PubMed] [Google Scholar]
  5. Leon M. A., Schwartz H. J. Inhibition of delayed hypersensitivity to tuberculin by concanavalin A. Proc Soc Exp Biol Med. 1969 Jul;131(3):735–736. doi: 10.3181/00379727-131-33964. [DOI] [PubMed] [Google Scholar]
  6. Markowitz H., Person D. A., Gitnick G. L., Ritts R. E., Jr Immunosuppressive activity of concanavalin A. Science. 1969 Jan 31;163(3866):476–476. doi: 10.1126/science.163.3866.476. [DOI] [PubMed] [Google Scholar]
  7. Perlmann P., Holm G. Cytotoxic effects of lymphoid cells in vitro. Adv Immunol. 1969;11:117–193. doi: 10.1016/s0065-2776(08)60479-4. [DOI] [PubMed] [Google Scholar]
  8. Perlmann P., Nilsson H., Leon M. A. Inhibition of cytotoxicity of lymphocytes by concanavalin A in vitro. Science. 1970 May 29;168(3935):1112–1115. doi: 10.1126/science.168.3935.1112. [DOI] [PubMed] [Google Scholar]
  9. Pick E., Krejcí J., Turk J. L. Release of skin reactive factor from guinea-pig lymphocytes by mitogens. Nature. 1970 Jan 17;225(5229):236–238. doi: 10.1038/225236a0. [DOI] [PubMed] [Google Scholar]
  10. Rosenau W. Target cell destruction. Fed Proc. 1968 Jan-Feb;27(1):34–38. [PubMed] [Google Scholar]
  11. Ruddle N. H., Waksman B. H. Cytotoxic effect of lymphocyte-antigen interaction in delayed hypersensitivity. Science. 1967 Sep 1;157(3792):1060–1062. doi: 10.1126/science.157.3792.1060. [DOI] [PubMed] [Google Scholar]
  12. Ruddle N. H., Waksman B. H. Cytotoxicity mediated by soluble antigen and lymphocytes in delayed hypersensitivity. 3. Analysis of mechanism. J Exp Med. 1968 Dec 1;128(6):1267–1279. doi: 10.1084/jem.128.6.1267. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Schlossman S. F., Levine H. Desensitization to delayed hypersensitivity reactions. With special reference to the requirement for an immunogenic molecule. J Immunol. 1967 Jul;99(1):111–114. [PubMed] [Google Scholar]
  14. Schwartz H. J., Leon M. A., Pelley R. P. Concanavalin A-induced release of skin-reactive factor from lymphoid cells. J Immunol. 1970 Jan;104(1):265–268. [PubMed] [Google Scholar]

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