Abstract
The host factor (HF-I) for phage Q beta RNA replication is a small protein of 102 amino acid residues encoded by the hfq gene at 94.8 min on the Escherichia coli chromosome. The synthesis rate of HF-I at the exponential-growth phase is higher than at the stationary phase, and it increases concomitantly with the increase in cell growth rate. The intracellular level of HF-I is about 30,000 to 60,000 molecules per cell, the majority being associated with ribosomes as one of the salt wash proteins. Taken together, we suggest that HF-I is one of the growth-related proteins.
Full text
PDFImages in this article
Selected References
These references are in PubMed. This may not be the complete list of references from this article.
- Blumenthal T., Carmichael G. G. RNA replication: function and structure of Qbeta-replicase. Annu Rev Biochem. 1979;48:525–548. doi: 10.1146/annurev.bi.48.070179.002521. [DOI] [PubMed] [Google Scholar]
- Carmichael G. G., Weber K., Niveleau A., Wahba A. J. The host factor required for RNA phage Qbeta RNA replication in vitro. Intracellular location, quantitation, and purification by polyadenylate-cellulose chromatography. J Biol Chem. 1975 May 25;250(10):3607–3612. [PubMed] [Google Scholar]
- DuBow M. S., Ryan T., Young R. A., Blumenthal T. Host factor for coliphage Q beta RNA replication: presence in procaryotes and association with the 30S ribosomal subunit in Escherichia coli. Mol Gen Genet. 1977 May 20;153(1):39–43. doi: 10.1007/BF01035994. [DOI] [PubMed] [Google Scholar]
- Eikhom T. S., Stockley D. J., Spiegelman S. Direct participation of a host protein in the replication of viral RNA in vitro. Proc Natl Acad Sci U S A. 1968 Feb;59(2):506–512. doi: 10.1073/pnas.59.2.506. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Enami M., Ishihama A. Biosynthesis of RNA polymerase in Escherichia coli. XII. Noncoordinate synthesis of core enzyme subunits after suppression of cell growth. Mol Gen Genet. 1982;185(3):373–378. doi: 10.1007/BF00334127. [DOI] [PubMed] [Google Scholar]
- Franze de Fernandez M. T., Eoyang L., August J. T. Factor fraction required for the synthesis of bacteriophage Qbeta-RNA. Nature. 1968 Aug 10;219(5154):588–590. doi: 10.1038/219588a0. [DOI] [PubMed] [Google Scholar]
- Franze de Fernandez M. T., Hayward W. S., August J. T. Bacterial proteins required for replication of phage Q ribonucleic acid. Pruification and properties of host factor I, a ribonucleic acid-binding protein. J Biol Chem. 1972 Feb 10;247(3):824–831. [PubMed] [Google Scholar]
- Haruna I., Spiegelman S. Recognition of size and sequence by an RNA replicase. Proc Natl Acad Sci U S A. 1965 Oct;54(4):1189–1193. doi: 10.1073/pnas.54.4.1189. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Igarashi K., Ishihama A. Bipartite functional map of the E. coli RNA polymerase alpha subunit: involvement of the C-terminal region in transcription activation by cAMP-CRP. Cell. 1991 Jun 14;65(6):1015–1022. doi: 10.1016/0092-8674(91)90553-b. [DOI] [PubMed] [Google Scholar]
- Iwakura Y., Ito K., Ishihama A. Biosynthesis of RNA polymerase in Escherichia coli. I. Control of RNA polymerase content at various growth rates. Mol Gen Genet. 1974;133(1):1–23. doi: 10.1007/BF00268673. [DOI] [PubMed] [Google Scholar]
- Kajitani M., Ishihama A. Identification and sequence determination of the host factor gene for bacteriophage Q beta. Nucleic Acids Res. 1991 Mar 11;19(5):1063–1066. doi: 10.1093/nar/19.5.1063. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Kamen R., Kondo M., Römer W., Weissmann C. Reconstitution of Q replicase lacking subunit with protein-synthesis-interference factor i. Eur J Biochem. 1972 Nov 21;31(1):44–51. doi: 10.1111/j.1432-1033.1972.tb02498.x. [DOI] [PubMed] [Google Scholar]
- Kawakami K., Saitoh T., Ishihama A. Biosynthesis of RNA polymerase in Escherichia coli. IX. Growth-dependent variations in the synthesis rate, content and distribution of RNA polymerase. Mol Gen Genet. 1979 Jul 13;174(2):107–116. doi: 10.1007/BF00268348. [DOI] [PubMed] [Google Scholar]
- Senear A. W., Steitz J. A. Site-specific interaction of Qbeta host factor and ribosomal protein S1 with Qbeta and R17 bacteriophage RNAs. J Biol Chem. 1976 Apr 10;251(7):1902–1912. [PubMed] [Google Scholar]
- Shapiro L., Franze de Fernandez M. T., August J. T. Resolution of two factors required in the Q-beta-RNA polymerase reaction. Nature. 1968 Nov 2;220(5166):478–480. doi: 10.1038/220478a0. [DOI] [PubMed] [Google Scholar]
- Wada A. Analysis of Escherichia coli ribosomal proteins by an improved two dimensional gel electrophoresis. I. Detection of four new proteins. J Biochem. 1986 Dec;100(6):1583–1594. doi: 10.1093/oxfordjournals.jbchem.a121866. [DOI] [PubMed] [Google Scholar]
- Wada A., Yamazaki Y., Fujita N., Ishihama A. Structure and probable genetic location of a "ribosome modulation factor" associated with 100S ribosomes in stationary-phase Escherichia coli cells. Proc Natl Acad Sci U S A. 1990 Apr;87(7):2657–2661. doi: 10.1073/pnas.87.7.2657. [DOI] [PMC free article] [PubMed] [Google Scholar]