Skip to main content
PMC home page
Journal of Bacteriology logoLink to Journal of Bacteriology
. 1994 Jan;176(2):540–542. doi: 10.1128/jb.176.2.540-542.1994

Mutations in the delta subunit influence the assembly of F1F0 ATP synthase in Escherichia coli.

A E Stack 1, B D Cain 1
PMCID: PMC205083  PMID: 8288552

Abstract

Missense mutations affecting Asp-161 and Ser-163 in the delta subunit of F1F0 ATP synthase have been generated. Although most substitutions allowed substantial enzyme function, the delta Asp-161-->Pro substitution resulted in a loss of enzyme activity. The loss of activity was attributable to a structural failure altering assembly of the enzyme complex.

Full text

PDF
541

Images in this article

542Image
on p.542

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Angov E., Ng T. C., Brusilow W. S. Effect of the delta subunit on assembly and proton permeability of the F0 proton channel of Escherichia coli F1F0 ATPase. J Bacteriol. 1991 Jan;173(1):407–411. doi: 10.1128/jb.173.1.407-411.1991. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Aris J. P., Klionsky D. J., Simoni R. D. The Fo subunits of the Escherichia coli F1Fo-ATP synthase are sufficient to form a functional proton pore. J Biol Chem. 1985 Sep 15;260(20):11207–11215. [PubMed] [Google Scholar]
  3. Cain B. D., Simoni R. D. Proton translocation by the F1F0ATPase of Escherichia coli. Mutagenic analysis of the a subunit. J Biol Chem. 1989 Feb 25;264(6):3292–3300. [PubMed] [Google Scholar]
  4. Deckers-Hebestreit G., Simoni R. D., Altendorf K. Influence of subunit-specific antibodies on the activity of the F0 complex of the ATP synthase of Escherichia coli. I. Effects of subunit b-specific polyclonal antibodies. J Biol Chem. 1992 Jun 15;267(17):12364–12369. [PubMed] [Google Scholar]
  5. Gogol E. P., Lücken U., Capaldi R. A. The stalk connecting the F1 and F0 domains of ATP synthase visualized by electron microscopy of unstained specimens. FEBS Lett. 1987 Jul 27;219(2):274–278. doi: 10.1016/0014-5793(87)80234-x. [DOI] [PubMed] [Google Scholar]
  6. Hartzog P. E., Cain B. D. Mutagenic analysis of the a subunit of the F1F0 ATP synthase in Escherichia coli: Gln-252 through Tyr-263. J Bacteriol. 1993 Mar;175(5):1337–1343. doi: 10.1128/jb.175.5.1337-1343.1993. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Hoesche J. A., Berzborn R. J. Primary structure, deduced from cDNA, secondary structure analysis and conclusions concerning interaction surfaces of the delta subunit of the photosynthetic ATP-synthase (E.C. 3.6.1.34) from millet (Sorghum bicolor) and maize (Zea mays). Biochim Biophys Acta. 1993 May 6;1142(3):293–305. doi: 10.1016/0005-2728(93)90157-b. [DOI] [PubMed] [Google Scholar]
  8. Humbert R., Brusilow W. S., Gunsalus R. P., Klionsky D. J., Simoni R. D. Escherichia coli mutants defective in the uncH gene. J Bacteriol. 1983 Jan;153(1):416–422. doi: 10.1128/jb.153.1.416-422.1983. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Jounouchi M., Takeyama M., Chaiprasert P., Noumi T., Moriyama Y., Maeda M., Futai M. Escherichia coli H(+)-ATPase: role of the delta subunit in binding Fl to the Fo sector. Arch Biochem Biophys. 1992 Feb 1;292(2):376–381. doi: 10.1016/0003-9861(92)90005-h. [DOI] [PubMed] [Google Scholar]
  10. Klionsky D. J., Brusilow W. S., Simoni R. D. Assembly of a functional F0 of the proton-translocating ATPase of Escherichia coli. J Biol Chem. 1983 Aug 25;258(16):10136–10143. [PubMed] [Google Scholar]
  11. Markwell M. A., Haas S. M., Bieber L. L., Tolbert N. E. A modification of the Lowry procedure to simplify protein determination in membrane and lipoprotein samples. Anal Biochem. 1978 Jun 15;87(1):206–210. doi: 10.1016/0003-2697(78)90586-9. [DOI] [PubMed] [Google Scholar]
  12. McCormick K. A., Cain B. D. Targeted mutagenesis of the b subunit of F1F0 ATP synthase in Escherichia coli: Glu-77 through Gln-85. J Bacteriol. 1991 Nov;173(22):7240–7248. doi: 10.1128/jb.173.22.7240-7248.1991. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Mendel-Hartvig J., Capaldi R. A. Structure-function relationships of domains of the delta subunit in Escherichia coli adenosine triphosphatase. Biochim Biophys Acta. 1991 Sep 27;1060(1):115–124. doi: 10.1016/s0005-2728(05)80126-4. [DOI] [PubMed] [Google Scholar]
  14. Monticello R. A., Angov E., Brusilow W. S. Effects of inducing expression of cloned genes for the F0 proton channel of the Escherichia coli F1F0 ATPase. J Bacteriol. 1992 May;174(10):3370–3376. doi: 10.1128/jb.174.10.3370-3376.1992. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Sternweis P. C., Smith J. B. Characterization of the purified membrane attachment (beta) subunit of the proton translocating adenosine triphosphatase from Escherichia coli. Biochemistry. 1977 Sep 6;16(18):4020–4025. doi: 10.1021/bi00637a013. [DOI] [PubMed] [Google Scholar]

Articles from Journal of Bacteriology are provided here courtesy of American Society for Microbiology (ASM)

RESOURCES