Skip to main content
Journal of Bacteriology logoLink to Journal of Bacteriology
. 1994 Apr;176(7):1997–2002. doi: 10.1128/jb.176.7.1997-2002.1994

Detailed structural characterization of succinoglycan, the major exopolysaccharide of Rhizobium meliloti Rm1021.

B B Reinhold 1, S Y Chan 1, T L Reuber 1, A Marra 1, G C Walker 1, V N Reinhold 1
PMCID: PMC205305  PMID: 8144468

Abstract

The detailed structure of the symbiotically important exopolysaccharide succinoglycan from Rhizobium meliloti Rm1021 was determined by mass spectrometry with electrospray ionization and collision-induced dissociation of the octameric oligosaccharide repeating unit. Previously undetermined locations of the succinyl and acetyl modifications were determined, in respect to both residue locations within the octamer and the carbon positions within the pyranose ring. Glycosidic linkages determined previously by methylation analysis were also verified.

Full text

PDF
1998

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Björndal H., Erbing C., Lindberg B., Fåhraeus G., Ljunggren H. Studies on an extracellular polysaccharide from Rhizobium meliloti. Acta Chem Scand. 1971;25(4):1281–1286. doi: 10.3891/acta.chem.scand.25-1281. [DOI] [PubMed] [Google Scholar]
  2. Fenn J. B., Mann M., Meng C. K., Wong S. F., Whitehouse C. M. Electrospray ionization for mass spectrometry of large biomolecules. Science. 1989 Oct 6;246(4926):64–71. doi: 10.1126/science.2675315. [DOI] [PubMed] [Google Scholar]
  3. Geyer R., Geyer H., Kühnhardt S., Mink W., Stirm S. Methylation analysis of complex carbohydrates in small amounts: capillary gas chromatography-mass fragmentography of methylalditol acetates obtained from N-glycosidically linked glycoprotein oligosaccharides. Anal Biochem. 1983 Aug;133(1):197–207. doi: 10.1016/0003-2697(83)90243-9. [DOI] [PubMed] [Google Scholar]
  4. Glazebrook J., Walker G. C. A novel exopolysaccharide can function in place of the calcofluor-binding exopolysaccharide in nodulation of alfalfa by Rhizobium meliloti. Cell. 1989 Feb 24;56(4):661–672. doi: 10.1016/0092-8674(89)90588-6. [DOI] [PubMed] [Google Scholar]
  5. Her G. R., Glazebrook J., Walker G. C., Reinhold V. N. Structural studies of a novel exopolysaccharide produced by a mutant of Rhizobium meliloti strain Rm1021. Carbohydr Res. 1990 May 1;198(2):305–312. doi: 10.1016/0008-6215(90)84300-j. [DOI] [PubMed] [Google Scholar]
  6. Jansson P. E., Kenne L., Lindberg B., Ljunggren H., Lönngren J., Rudén U., Svensson S. Demonstration of an octasaccharide repeating unit in the extracellular polysaccharide of Rhizobium meliloti by sequential degradation. J Am Chem Soc. 1977 May 25;99(11):3812–3815. doi: 10.1021/ja00453a049. [DOI] [PubMed] [Google Scholar]
  7. Leigh J. A., Coplin D. L. Exopolysaccharides in plant-bacterial interactions. Annu Rev Microbiol. 1992;46:307–346. doi: 10.1146/annurev.mi.46.100192.001515. [DOI] [PubMed] [Google Scholar]
  8. Leigh J. A., Reed J. W., Hanks J. F., Hirsch A. M., Walker G. C. Rhizobium meliloti mutants that fail to succinylate their calcofluor-binding exopolysaccharide are defective in nodule invasion. Cell. 1987 Nov 20;51(4):579–587. doi: 10.1016/0092-8674(87)90127-9. [DOI] [PubMed] [Google Scholar]
  9. Leigh J. A., Signer E. R., Walker G. C. Exopolysaccharide-deficient mutants of Rhizobium meliloti that form ineffective nodules. Proc Natl Acad Sci U S A. 1985 Sep;82(18):6231–6235. doi: 10.1073/pnas.82.18.6231. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Reuber T. L., Walker G. C. The acetyl substituent of succinoglycan is not necessary for alfalfa nodule invasion by Rhizobium meliloti Rm1021. J Bacteriol. 1993 Jun;175(11):3653–3655. doi: 10.1128/jb.175.11.3653-3655.1993. [DOI] [PMC free article] [PubMed] [Google Scholar]

Articles from Journal of Bacteriology are provided here courtesy of American Society for Microbiology (ASM)

RESOURCES