Skip to main content
PMC home page
Journal of Bacteriology logoLink to Journal of Bacteriology
. 1994 Apr;176(7):2133–2135. doi: 10.1128/jb.176.7.2133-2135.1994

Reconstruction of glucose uptake and phosphorylation in a glucose-negative mutant of Escherichia coli by using Zymomonas mobilis genes encoding the glucose facilitator protein and glucokinase.

J L Snoep 1, N Arfman 1, L P Yomano 1, R K Fliege 1, T Conway 1, L O Ingram 1
PMCID: PMC205325  PMID: 8144485

Abstract

Expression of the Zymomonas mobilis glf (glucose facilitator protein) and glk (glucokinase) genes in Escherichia coli ZSC113 (glucose negative) provided a new functional pathway for glucose uptake and phosphorylation. Both genes were essential for the restoration of growth in glucose minimal medium and for acid production on glucose-MacConkey agar plates.

Full text

PDF
2134

Images in this article

2134Image
on p.2134
2134Image
on p.2134

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. An H., Scopes R. K., Rodriguez M., Keshav K. F., Ingram L. O. Gel electrophoretic analysis of Zymomonas mobilis glycolytic and fermentative enzymes: identification of alcohol dehydrogenase II as a stress protein. J Bacteriol. 1991 Oct;173(19):5975–5982. doi: 10.1128/jb.173.19.5975-5982.1991. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Arfman N., Worrell V., Ingram L. O. Use of the tac promoter and lacIq for the controlled expression of Zymomonas mobilis fermentative genes in Escherichia coli and Zymomonas mobilis. J Bacteriol. 1992 Nov;174(22):7370–7378. doi: 10.1128/jb.174.22.7370-7378.1992. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Barnell W. O., Liu J., Hesman T. L., O'Neill M. C., Conway T. The Zymomonas mobilis glf, zwf, edd, and glk genes form an operon: localization of the promoter and identification of a conserved sequence in the regulatory region. J Bacteriol. 1992 May;174(9):2816–2823. doi: 10.1128/jb.174.9.2816-2823.1992. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Barnell W. O., Yi K. C., Conway T. Sequence and genetic organization of a Zymomonas mobilis gene cluster that encodes several enzymes of glucose metabolism. J Bacteriol. 1990 Dec;172(12):7227–7240. doi: 10.1128/jb.172.12.7227-7240.1990. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Belaich J. P., Senez J. C., Murgier M. Microcalorimetric study of glucose permeation in microbial cells. J Bacteriol. 1968 May;95(5):1750–1757. doi: 10.1128/jb.95.5.1750-1757.1968. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Bisson L. F., Fraenkel D. G. Involvement of kinases in glucose and fructose uptake by Saccharomyces cerevisiae. Proc Natl Acad Sci U S A. 1983 Mar;80(6):1730–1734. doi: 10.1073/pnas.80.6.1730. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Bradford M. M. A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal Biochem. 1976 May 7;72:248–254. doi: 10.1006/abio.1976.9999. [DOI] [PubMed] [Google Scholar]
  8. Carey V. C., Ingram L. O. Lipid composition of Zymomonas mobilis: effects of ethanol and glucose. J Bacteriol. 1983 Jun;154(3):1291–1300. doi: 10.1128/jb.154.3.1291-1300.1983. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Curtis S. J., Epstein W. Phosphorylation of D-glucose in Escherichia coli mutants defective in glucosephosphotransferase, mannosephosphotransferase, and glucokinase. J Bacteriol. 1975 Jun;122(3):1189–1199. doi: 10.1128/jb.122.3.1189-1199.1975. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Fliege R., Tong S., Shibata A., Nickerson K. W., Conway T. The Entner-Doudoroff pathway in Escherichia coli is induced for oxidative glucose metabolism via pyrroloquinoline quinone-dependent glucose dehydrogenase. Appl Environ Microbiol. 1992 Dec;58(12):3826–3829. doi: 10.1128/aem.58.12.3826-3829.1992. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Hommes R. W., Simons J. A., Snoep J. L., Postma P. W., Tempest D. W., Neijssel O. M. Quantitative aspects of glucose metabolism by Escherichia coli B/r, grown in the presence of pyrroloquinoline quinone. Antonie Van Leeuwenhoek. 1991 Oct-Nov;60(3-4):373–382. doi: 10.1007/BF00430375. [DOI] [PubMed] [Google Scholar]
  12. Postma P. W., Lengeler J. W., Jacobson G. R. Phosphoenolpyruvate:carbohydrate phosphotransferase systems of bacteria. Microbiol Rev. 1993 Sep;57(3):543–594. doi: 10.1128/mr.57.3.543-594.1993. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Sahm H., Rohmer M., Bringer-Meyer S., Sprenger G. A., Welle R. Biochemistry and physiology of hopanoids in bacteria. Adv Microb Physiol. 1993;35:247–273. doi: 10.1016/s0065-2911(08)60100-9. [DOI] [PubMed] [Google Scholar]
  14. van Schie B. J., Hellingwerf K. J., van Dijken J. P., Elferink M. G., van Dijl J. M., Kuenen J. G., Konings W. N. Energy transduction by electron transfer via a pyrrolo-quinoline quinone-dependent glucose dehydrogenase in Escherichia coli, Pseudomonas aeruginosa, and Acinetobacter calcoaceticus (var. lwoffi). J Bacteriol. 1985 Aug;163(2):493–499. doi: 10.1128/jb.163.2.493-499.1985. [DOI] [PMC free article] [PubMed] [Google Scholar]

Articles from Journal of Bacteriology are provided here courtesy of American Society for Microbiology (ASM)

RESOURCES