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. 1994 Jun;176(11):3403–3407. doi: 10.1128/jb.176.11.3403-3407.1994

Cloning and characterization of the Inc A/C plasmid RA1 replicon.

C Llanes 1, P Gabant 1, M Couturier 1, Y Michel-Briand 1
PMCID: PMC205517  PMID: 8195101

Abstract

The Inc A/C plasmids, like Inc P and Inc Q plasmids, have a broad host range. However, their maintenance functions remain to be studied. An autoreplicative region of 2.79 kb named RepA/C, able to replicate both in the family Enterobacteriaceae and in Pseudomonas spp., was isolated and sequenced. The stability, copy number, and incompatibility expression of this replicon were determined. RepA/C and a nonautoreplicative fragment of 16 kb of this replicon were used as probes and showed specific hybridizations with the Inc P3-A/C plasmids from Pseudomonas spp. and members of the Enterobacteriaceae. These probes could be used as tools for identification of the plasmids of this epidemiologically important Inc group.

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Selected References

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