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. 1994 Jun;176(11):3403–3407. doi: 10.1128/jb.176.11.3403-3407.1994

Cloning and characterization of the Inc A/C plasmid RA1 replicon.

C Llanes 1, P Gabant 1, M Couturier 1, Y Michel-Briand 1
PMCID: PMC205517  PMID: 8195101

Abstract

The Inc A/C plasmids, like Inc P and Inc Q plasmids, have a broad host range. However, their maintenance functions remain to be studied. An autoreplicative region of 2.79 kb named RepA/C, able to replicate both in the family Enterobacteriaceae and in Pseudomonas spp., was isolated and sequenced. The stability, copy number, and incompatibility expression of this replicon were determined. RepA/C and a nonautoreplicative fragment of 16 kb of this replicon were used as probes and showed specific hybridizations with the Inc P3-A/C plasmids from Pseudomonas spp. and members of the Enterobacteriaceae. These probes could be used as tools for identification of the plasmids of this epidemiologically important Inc group.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Aoki T., Egusa S., Ogata Y., Watanabe T. Detection of resistance factors in fish pathogen Aeromonas liquefaciens. J Gen Microbiol. 1971 Mar;65(3):343–349. doi: 10.1099/00221287-65-3-343. [DOI] [PubMed] [Google Scholar]
  2. Bradley D. E. Conjugation system of IncC plasmid RA1, and the interaction of RA1 pili with specific RNA phage C-1. Res Microbiol. 1989 Sep;140(7):439–446. doi: 10.1016/0923-2508(89)90064-8. [DOI] [PubMed] [Google Scholar]
  3. Chattoraj D. K., Mason R. J., Wickner S. H. Mini-P1 plasmid replication: the autoregulation-sequestration paradox. Cell. 1988 Feb 26;52(4):551–557. doi: 10.1016/0092-8674(88)90468-0. [DOI] [PubMed] [Google Scholar]
  4. Couturier M., Bex F., Bergquist P. L., Maas W. K. Identification and classification of bacterial plasmids. Microbiol Rev. 1988 Sep;52(3):375–395. doi: 10.1128/mr.52.3.375-395.1988. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Fang F. C., Helinski D. R. Broad-host-range properties of plasmid RK2: importance of overlapping genes encoding the plasmid replication initiation protein TrfA. J Bacteriol. 1991 Sep;173(18):5861–5868. doi: 10.1128/jb.173.18.5861-5868.1991. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Griffin H. G., Foster T. J., Falkiner F. R., Carr M. E., Coleman D. C. Molecular analysis of multiple-resistance plasmids transferred from gram-negative bacteria isolated in a urological unit. Antimicrob Agents Chemother. 1985 Sep;28(3):413–418. doi: 10.1128/aac.28.3.413. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Hedges R. W., Rodriguez-Lemoine V., Datta N. R factors from Serratia marcescens. J Gen Microbiol. 1975 Jan;86(1):88–92. doi: 10.1099/00221287-86-1-88. [DOI] [PubMed] [Google Scholar]
  8. Kieser T. Factors affecting the isolation of CCC DNA from Streptomyces lividans and Escherichia coli. Plasmid. 1984 Jul;12(1):19–36. doi: 10.1016/0147-619x(84)90063-5. [DOI] [PubMed] [Google Scholar]
  9. Kües U., Stahl U. Replication of plasmids in gram-negative bacteria. Microbiol Rev. 1989 Dec;53(4):491–516. doi: 10.1128/mr.53.4.491-516.1989. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Lin L. S., Kim Y. J., Meyer R. J. The 20 bp, directly repeated DNA sequence of broad host range plasmid R1162 exerts incompatibility in vivo and inhibits R1162 DNA replication in vitro. Mol Gen Genet. 1987 Jul;208(3):390–397. doi: 10.1007/BF00328129. [DOI] [PubMed] [Google Scholar]
  11. Llanes C., Couturier M., Asfeld L., Grimont F., Michel-Briand Y. Replicon typing of 71 multiresistant Serratia marcescens strains. Res Microbiol. 1994 Jan;145(1):17–25. doi: 10.1016/0923-2508(94)90063-9. [DOI] [PubMed] [Google Scholar]
  12. Manen D., Upegui-Gonzalez L. C., Caro L. Monomers and dimers of the RepA protein in plasmid pSC101 replication: domains in RepA. Proc Natl Acad Sci U S A. 1992 Oct 1;89(19):8923–8927. doi: 10.1073/pnas.89.19.8923. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Martínez-Salazar J. M., Gómez-Eichelmann M. C. Molecular rearrangements between two plasmids of the FII incompatibility group in different recombination--deficient Escherichia coli strains. Plasmid. 1987 Nov;18(3):237–245. doi: 10.1016/0147-619x(87)90066-7. [DOI] [PubMed] [Google Scholar]
  14. Nordström K., Austin S. J. Mechanisms that contribute to the stable segregation of plasmids. Annu Rev Genet. 1989;23:37–69. doi: 10.1146/annurev.ge.23.120189.000345. [DOI] [PubMed] [Google Scholar]
  15. Perri S., Helinski D. R., Toukdarian A. Interactions of plasmid-encoded replication initiation proteins with the origin of DNA replication in the broad host range plasmid RK2. J Biol Chem. 1991 Jul 5;266(19):12536–12543. [PubMed] [Google Scholar]
  16. Roberts R. C., Helinski D. R. Definition of a minimal plasmid stabilization system from the broad-host-range plasmid RK2. J Bacteriol. 1992 Dec;174(24):8119–8132. doi: 10.1128/jb.174.24.8119-8132.1992. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Sadler J. R., Tecklenburg M., Betz J. L. Plasmids containing many tandem copies of a synthetic lactose operator. Gene. 1980 Feb;8(3):279–300. doi: 10.1016/0378-1119(80)90005-0. [DOI] [PubMed] [Google Scholar]
  18. Sanger F., Nicklen S., Coulson A. R. DNA sequencing with chain-terminating inhibitors. Proc Natl Acad Sci U S A. 1977 Dec;74(12):5463–5467. doi: 10.1073/pnas.74.12.5463. [DOI] [PMC free article] [PubMed] [Google Scholar]
  19. Sayre P., Miller R. V. Bacterial mobile genetic elements: importance in assessing the environmental fate of genetically engineered sequences. Plasmid. 1991 Nov;26(3):151–171. doi: 10.1016/0147-619x(91)90040-4. [DOI] [PubMed] [Google Scholar]
  20. Schaefer C., Messer W. DnaA protein/DNA interaction. Modulation of the recognition sequence. Mol Gen Genet. 1991 Apr;226(1-2):34–40. doi: 10.1007/BF00273584. [DOI] [PubMed] [Google Scholar]
  21. Smith C. A., Thomas C. M. Comparison of the nucleotide sequences of the vegetative replication origins of broad host range IncP plasmids R751 and RK2 reveals conserved features of probable functional importance. Nucleic Acids Res. 1985 Jan 25;13(2):557–572. doi: 10.1093/nar/13.2.557. [DOI] [PMC free article] [PubMed] [Google Scholar]
  22. Smith C. A., Thomas C. M. Comparison of the organisation of the genomes of phenotypically diverse plasmids of incompatibility group P: members of the IncP beta sub-group are closely related. Mol Gen Genet. 1987 Mar;206(3):419–427. doi: 10.1007/BF00428881. [DOI] [PubMed] [Google Scholar]
  23. Stalker D. M., Thomas C. M., Helinski D. R. Nucleotide sequence of the region of the origin of replication of the broad host range plasmid RK2. Mol Gen Genet. 1981;181(1):8–12. doi: 10.1007/BF00338997. [DOI] [PubMed] [Google Scholar]
  24. Thomas C. M., Meyer R., Helinski D. R. Regions of broad-host-range plasmid RK2 which are essential for replication and maintenance. J Bacteriol. 1980 Jan;141(1):213–222. doi: 10.1128/jb.141.1.213-222.1980. [DOI] [PMC free article] [PubMed] [Google Scholar]
  25. Van der Plas J., Oosterhoff-Teertstra R., Borrias M., Weisbeek P. Identification of replication and stability functions in the complete nucleotide sequence of plasmid pUH24 from the cyanobacterium Synechococcus sp. PCC 7942. Mol Microbiol. 1992 Mar;6(5):653–664. doi: 10.1111/j.1365-2958.1992.tb01513.x. [DOI] [PubMed] [Google Scholar]
  26. Vanooteghem J. C., Cornelis G. R. Structural and functional similarities between the replication region of the Yersinia virulence plasmid and the RepFIIA replicons. J Bacteriol. 1990 Jul;172(7):3600–3608. doi: 10.1128/jb.172.7.3600-3608.1990. [DOI] [PMC free article] [PubMed] [Google Scholar]
  27. Wickner S., Hoskins J., McKenney K. Monomerization of RepA dimers by heat shock proteins activates binding to DNA replication origin. Proc Natl Acad Sci U S A. 1991 Sep 15;88(18):7903–7907. doi: 10.1073/pnas.88.18.7903. [DOI] [PMC free article] [PubMed] [Google Scholar]

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