Skip to main content
PMC home page
Journal of Bacteriology logoLink to Journal of Bacteriology
. 1994 Jul;176(14):4459–4462. doi: 10.1128/jb.176.14.4459-4462.1994

Transformation of Mycoplasma gallisepticum with Tn916, Tn4001, and integrative plasmid vectors.

J Cao 1, P A Kapke 1, F C Minion 1
PMCID: PMC205662  PMID: 8021232

Abstract

Mycoplasma gallisepticum causes respiratory disease in avian species, but little is known about its mechanism(s) of pathogenesis. These studies were undertaken in order to develop genetic systems for analysis of potential virulence factors. M. gallisepticum was transformed with plasmids containing one of the gram-positive transposons Tn916 or Tn4001, which inserted randomly into the mycoplasmal chromosome. Plasmids containing cloned chromosomal DNA were also constructed and tested for integration into regions of DNA homology derived either from chromosomal fragments or from the gentamicin resistance marker from Tn4001. These studies demonstrate that M. gallisepticum is amenable to transformation with both transposons and integrative vectors.

Full text

PDF
4461

Images in this article

4460Image
on p.4460
4461Image
on p.4461

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Birnboim H. C. A rapid alkaline extraction method for the isolation of plasmid DNA. Methods Enzymol. 1983;100:243–255. doi: 10.1016/0076-6879(83)00059-2. [DOI] [PubMed] [Google Scholar]
  2. Bove J. M., Candresse T., Mouches C., Renaudin J., Saillard C. Spiroplasmas and the transfer of genetic material by transformation and transfection. Isr J Med Sci. 1984 Sep;20(9):836–839. [PubMed] [Google Scholar]
  3. Dybvig K., Alderete J. Transformation of Mycoplasma pulmonis and Mycoplasma hyorhinis: transposition of Tn916 and formation of cointegrate structures. Plasmid. 1988 Jul;20(1):33–41. doi: 10.1016/0147-619x(88)90005-4. [DOI] [PubMed] [Google Scholar]
  4. Dybvig K., Cassell G. H. Transposition of gram-positive transposon Tn916 in Acholeplasma laidlawii and Mycoplasma pulmonis. Science. 1987 Mar 13;235(4794):1392–1394. doi: 10.1126/science.3029869. [DOI] [PubMed] [Google Scholar]
  5. Dybvig K. Transformation of Acholeplasma laidlawii with streptococcal plasmids pVA868 and pVA920. Plasmid. 1989 Mar;21(2):155–160. doi: 10.1016/0147-619x(89)90061-9. [DOI] [PubMed] [Google Scholar]
  6. Gawron-Burke C., Clewell D. B. A transposon in Streptococcus faecalis with fertility properties. Nature. 1982 Nov 18;300(5889):281–284. doi: 10.1038/300281a0. [DOI] [PubMed] [Google Scholar]
  7. Hedreyda C. T., Lee K. K., Krause D. C. Transformation of Mycoplasma pneumoniae with Tn4001 by electroporation. Plasmid. 1993 Sep;30(2):170–175. doi: 10.1006/plas.1993.1047. [DOI] [PubMed] [Google Scholar]
  8. King K. W., Dybvig K. Plasmid transformation of Mycoplasma mycoides subspecies mycoides is promoted by high concentrations of polyethylene glycol. Plasmid. 1991 Sep;26(2):108–115. doi: 10.1016/0147-619x(91)90050-7. [DOI] [PubMed] [Google Scholar]
  9. Knudtson K. L., Minion F. C. Construction of Tn4001lac derivatives to be used as promoter probe vectors in mycoplasmas. Gene. 1993 Dec 31;137(2):217–222. doi: 10.1016/0378-1119(93)90009-r. [DOI] [PubMed] [Google Scholar]
  10. Mahairas G. G., Jian C., Minion F. C. Development of a cloning system in Mycoplasma pulmonis. Gene. 1990 Sep 1;93(1):61–66. doi: 10.1016/0378-1119(90)90136-f. [DOI] [PubMed] [Google Scholar]
  11. Mahairas G. G., Minion F. C. Random insertion of the gentamicin resistance transposon Tn4001 in Mycoplasma pulmonis. Plasmid. 1989 Jan;21(1):43–47. doi: 10.1016/0147-619x(89)90085-1. [DOI] [PubMed] [Google Scholar]
  12. Mahairas G. G., Minion F. C. Transformation of Mycoplasma pulmonis: demonstration of homologous recombination, introduction of cloned genes, and preliminary description of an integrating shuttle system. J Bacteriol. 1989 Apr;171(4):1775–1780. doi: 10.1128/jb.171.4.1775-1780.1989. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Nascimento E. R., Yamamoto R., Herrick K. R., Tait R. C. Polymerase chain reaction for detection of Mycoplasma gallisepticum. Avian Dis. 1991 Jan-Mar;35(1):62–69. [PubMed] [Google Scholar]
  14. Roberts M. C., Kenny G. E. Conjugal transfer of transposon Tn916 from Streptococcus faecalis to Mycoplasma hominis. J Bacteriol. 1987 Aug;169(8):3836–3839. doi: 10.1128/jb.169.8.3836-3839.1987. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Salyers A. A., Speer B. S., Shoemaker N. B. New perspectives in tetracycline resistance. Mol Microbiol. 1990 Jan;4(1):151–156. doi: 10.1111/j.1365-2958.1990.tb02025.x. [DOI] [PubMed] [Google Scholar]
  16. Stamburski C., Renaudin J., Bove J. M. First step toward a virus-derived vector for gene cloning and expression in spiroplasmas, organisms which read UGA as a tryptophan codon: synthesis of chloramphenicol acetyltransferase in Spiroplasma citri. J Bacteriol. 1991 Apr;173(7):2225–2230. doi: 10.1128/jb.173.7.2225-2230.1991. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Whitley J. C., Finch L. R. Location of sites of transposon Tn916 insertion in the Mycoplasma mycoides genome. J Bacteriol. 1989 Dec;171(12):6870–6872. doi: 10.1128/jb.171.12.6870-6872.1989. [DOI] [PMC free article] [PubMed] [Google Scholar]

Articles from Journal of Bacteriology are provided here courtesy of American Society for Microbiology (ASM)

RESOURCES