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. 1992 Jan;174(2):377–383. doi: 10.1128/jb.174.2.377-383.1992

Characterization of Aspergillus nidulans mutants deficient in cell wall chitin or glucan.

P T Borgia 1, C L Dodge 1
PMCID: PMC205727  PMID: 1729232

Abstract

By screening for the osmotically remediable phenotype, mutations in two genes (orlA and orlB) affecting the cell wall chitin content of Aspergillus nidulans were identified. Strains carrying temperature-sensitive alleles of these genes produce conidia which swell excessively and lyse when germinated at restrictive temperatures. Growth under these conditions is remedied by osmotic stabilizers and by N-acetylglucosamine (GlcNAc). Remediation by GlcNAc suggests that the mutations affect early steps in the synthesis of chitin. Temperature and medium shift experiments indicate that the phenotype is the result of decreased synthesis rather than increased chitin degradation and that osmotic stabilizers act to stabilize a defective wall rather than to stabilize the gene product. Two genes, orlC and orlD, which affect cell wall beta-1,3-glucan content were also identified. Walls from strains carrying mutations in these genes exhibit normal amounts of alpha-1,3-glucan and chitin but reduced amounts of beta-1,3-glucan. As for the chitin-deficient mutants, orlC and orlD mutants spontaneously lyse on conventional media but are remedied by osmotic stabilizers. These results indicate that both chitin and beta-1,3-glucan are likely to contribute to the structural rigidity of the cell wall.

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Selected References

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  1. Au-Young J., Robbins P. W. Isolation of a chitin synthase gene (CHS1) from Candida albicans by expression in Saccharomyces cerevisiae. Mol Microbiol. 1990 Feb;4(2):197–207. doi: 10.1111/j.1365-2958.1990.tb00587.x. [DOI] [PubMed] [Google Scholar]
  2. Bartnicki-Garcia S. Cell wall chemistry, morphogenesis, and taxonomy of fungi. Annu Rev Microbiol. 1968;22:87–108. doi: 10.1146/annurev.mi.22.100168.000511. [DOI] [PubMed] [Google Scholar]
  3. Borgia P. T. Roles of the orlA, tsE, and bimG genes of Aspergillus nidulans in chitin synthesis. J Bacteriol. 1992 Jan;174(2):384–389. doi: 10.1128/jb.174.2.384-389.1992. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Bulawa C. E., Osmond B. C. Chitin synthase I and chitin synthase II are not required for chitin synthesis in vivo in Saccharomyces cerevisiae. Proc Natl Acad Sci U S A. 1990 Oct;87(19):7424–7428. doi: 10.1073/pnas.87.19.7424. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Bulawa C. E., Slater M., Cabib E., Au-Young J., Sburlati A., Adair W. L., Jr, Robbins P. W. The S. cerevisiae structural gene for chitin synthase is not required for chitin synthesis in vivo. Cell. 1986 Jul 18;46(2):213–225. doi: 10.1016/0092-8674(86)90738-5. [DOI] [PubMed] [Google Scholar]
  6. Bull A. T. Chemical composition of wild-type and mutant Aspergillus nidulans cell walls. The nature of polysaccharide and melanin constituents. J Gen Microbiol. 1970 Sep;63(1):75–94. doi: 10.1099/00221287-63-1-75. [DOI] [PubMed] [Google Scholar]
  7. Cabib E., Bowers B. Chitin and yeast budding. Localization of chitin in yeast bud scars. J Biol Chem. 1971 Jan 10;246(1):152–159. [PubMed] [Google Scholar]
  8. Cabib E., Roberts R., Bowers B. Synthesis of the yeast cell wall and its regulation. Annu Rev Biochem. 1982;51:763–793. doi: 10.1146/annurev.bi.51.070182.003555. [DOI] [PubMed] [Google Scholar]
  9. Cabib E., Sburlati A., Bowers B., Silverman S. J. Chitin synthase 1, an auxiliary enzyme for chitin synthesis in Saccharomyces cerevisiae. J Cell Biol. 1989 May;108(5):1665–1672. doi: 10.1083/jcb.108.5.1665. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Katz D., Rosenberger R. F. A mutation in Aspergillus nidulans producing hyphal walls which lack chitin. Biochim Biophys Acta. 1970 Jun;208(3):452–460. doi: 10.1016/0304-4165(70)90218-7. [DOI] [PubMed] [Google Scholar]
  11. Marshall M. A., Timberlake W. E. Aspergillus nidulans wetA activates spore-specific gene expression. Mol Cell Biol. 1991 Jan;11(1):55–62. doi: 10.1128/mcb.11.1.55. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Martinelli S. D., Clutterbuck A. J. A quantitative survey of conidiation mutants in Aspergillus nidulans. J Gen Microbiol. 1971 Dec;69(2):261–268. doi: 10.1099/00221287-69-2-261. [DOI] [PubMed] [Google Scholar]
  13. Polacheck I., Rosenberger R. F. Aspergillus nidulans mutant lacking alpha-(1,3)-glucan, melanin, and cleistothecia. J Bacteriol. 1977 Nov;132(2):650–656. doi: 10.1128/jb.132.2.650-656.1977. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Roncero C., Valdivieso M. H., Ribas J. C., Durán A. Isolation and characterization of Saccharomyces cerevisiae mutants resistant to Calcofluor white. J Bacteriol. 1988 Apr;170(4):1950–1954. doi: 10.1128/jb.170.4.1950-1954.1988. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Sewall T. C., Mims C. W., Timberlake W. E. Conidium differentiation in Aspergillus nidulans wild-type and wet-white (wetA) mutant strains. Dev Biol. 1990 Apr;138(2):499–508. doi: 10.1016/0012-1606(90)90215-5. [DOI] [PubMed] [Google Scholar]
  16. Shaw J. A., Mol P. C., Bowers B., Silverman S. J., Valdivieso M. H., Durán A., Cabib E. The function of chitin synthases 2 and 3 in the Saccharomyces cerevisiae cell cycle. J Cell Biol. 1991 Jul;114(1):111–123. doi: 10.1083/jcb.114.1.111. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Shepherd M. G. Cell envelope of Candida albicans. Crit Rev Microbiol. 1987;15(1):7–25. doi: 10.3109/10408418709104445. [DOI] [PubMed] [Google Scholar]
  18. Silverman S. J., Sburlati A., Slater M. L., Cabib E. Chitin synthase 2 is essential for septum formation and cell division in Saccharomyces cerevisiae. Proc Natl Acad Sci U S A. 1988 Jul;85(13):4735–4739. doi: 10.1073/pnas.85.13.4735. [DOI] [PMC free article] [PubMed] [Google Scholar]
  19. Silverman S. J. Similar and different domains of chitin synthases 1 and 2 of S. cerevisiae: two isozymes with distinct functions. Yeast. 1989 Nov-Dec;5(6):459–467. doi: 10.1002/yea.320050605. [DOI] [PubMed] [Google Scholar]
  20. Surarit R., Gopal P. K., Shepherd M. G. Evidence for a glycosidic linkage between chitin and glucan in the cell wall of Candida albicans. J Gen Microbiol. 1988 Jun;134(6):1723–1730. doi: 10.1099/00221287-134-6-1723. [DOI] [PubMed] [Google Scholar]
  21. Valdivieso M. H., Mol P. C., Shaw J. A., Cabib E., Durán A. CAL1, a gene required for activity of chitin synthase 3 in Saccharomyces cerevisiae. J Cell Biol. 1991 Jul;114(1):101–109. doi: 10.1083/jcb.114.1.101. [DOI] [PMC free article] [PubMed] [Google Scholar]
  22. Zonneveld B. J. Alpha-1,3 glucan synthesis correlated with alpha-1,3 glucanase synthesis, conidiation and fructification in morphogenetic mutants of Aspergillus nidulans. J Gen Microbiol. 1974 Apr;81(2):445–451. doi: 10.1099/00221287-81-2-445. [DOI] [PubMed] [Google Scholar]
  23. Zonneveld B. J. Biochemical analysis of the cell wall of Aspergillus nidulans. Biochim Biophys Acta. 1971 Dec 3;249(2):506–514. doi: 10.1016/0005-2736(71)90126-x. [DOI] [PubMed] [Google Scholar]

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