Skip to main content
PMC home page
Journal of Bacteriology logoLink to Journal of Bacteriology
. 1992 Jan;174(2):434–440. doi: 10.1128/jb.174.2.434-440.1992

Phosphoglycerate mutase from Streptomyces coelicolor A3(2): purification and characterization of the enzyme and cloning and sequence analysis of the gene.

P J White 1, J Nairn 1, N C Price 1, H G Nimmo 1, J R Coggins 1, I S Hunter 1
PMCID: PMC205734  PMID: 1530847

Abstract

The enzyme 3-phosphoglycerate mutase was purified 192-fold from Streptomyces coelicolor, and its N-terminal sequence was determined. The enzyme is tetrameric with a subunit Mr of 29,000. It is 2,3-bisphosphoglycerate dependent and inhibited by vanadate. The gene encoding the enzyme was cloned by using a synthetic oligonucleotide probe designed from the N-terminal peptide sequence, and the complete coding sequence was determined. The deduced amino acid sequence is 64% identical to that of the phosphoglycerate mutase of Saccharomyces cerevisiae and has substantial identity to those of other phosphoglycerate mutases.

Full text

PDF
436

Images in this article

435Image
on p.435

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Bibb M. J., Findlay P. R., Johnson M. W. The relationship between base composition and codon usage in bacterial genes and its use for the simple and reliable identification of protein-coding sequences. Gene. 1984 Oct;30(1-3):157–166. doi: 10.1016/0378-1119(84)90116-1. [DOI] [PubMed] [Google Scholar]
  2. Carreras J., Bartrons R., Grisolía S. Vanadate inhibits 2,3-bisphosphoglycerate dependent phosphoglycerate mutases but does not affect the 2,3-bisphosphoglycerate independent phosphoglycerate mutases. Biochem Biophys Res Commun. 1980 Oct 16;96(3):1267–1273. doi: 10.1016/0006-291x(80)90088-1. [DOI] [PubMed] [Google Scholar]
  3. Castellà-Escolà J., Montoliu L., Pons G., Puigdomènech P., Cohen-Solal M., Carreras J., Rigau J., Climent F. Sequence of rat skeletal muscle phosphoglycerate mutase cDNA. Biochem Biophys Res Commun. 1989 Dec 29;165(3):1345–1351. doi: 10.1016/0006-291x(89)92751-4. [DOI] [PubMed] [Google Scholar]
  4. Devereux J., Haeberli P., Smithies O. A comprehensive set of sequence analysis programs for the VAX. Nucleic Acids Res. 1984 Jan 11;12(1 Pt 1):387–395. doi: 10.1093/nar/12.1part1.387. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Hermann R., Rudolph R., Jaenicke R., Price N. C., Scobbie A. The reconstitution of denatured phosphoglycerate mutase. J Biol Chem. 1983 Sep 25;258(18):11014–11019. [PubMed] [Google Scholar]
  6. Johnson C. M., Price N. C. Denaturation and renaturation of the monomeric phosphoglycerate mutase from Schizosaccharomyces pombe. Biochem J. 1987 Jul 15;245(2):525–530. doi: 10.1042/bj2450525. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Joulin V., Peduzzi J., Roméo P. H., Rosa R., Valentin C., Dubart A., Lapeyre B., Blouquit Y., Garel M. C., Goossens M. Molecular cloning and sequencing of the human erythrocyte 2,3-bisphosphoglycerate mutase cDNA: revised amino acid sequence. EMBO J. 1986 Sep;5(9):2275–2283. doi: 10.1002/j.1460-2075.1986.tb04495.x. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Laemmli U. K. Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature. 1970 Aug 15;227(5259):680–685. doi: 10.1038/227680a0. [DOI] [PubMed] [Google Scholar]
  9. Lawlor E. J., Baylis H. A., Chater K. F. Pleiotropic morphological and antibiotic deficiencies result from mutations in a gene encoding a tRNA-like product in Streptomyces coelicolor A3(2). Genes Dev. 1987 Dec;1(10):1305–1310. doi: 10.1101/gad.1.10.1305. [DOI] [PubMed] [Google Scholar]
  10. Le Boulch P., Joulin V., Garel M. C., Rosa J., Cohen-Solal M. Molecular cloning and nucleotide sequence of murine 2,3-bisphosphoglycerate mutase cDNA. Biochem Biophys Res Commun. 1988 Oct 31;156(2):874–881. doi: 10.1016/s0006-291x(88)80925-2. [DOI] [PubMed] [Google Scholar]
  11. Price N. C., Duncan D., Ogg D. J. Purification and preliminary characterization of phosphoglycerate mutase from Schizosaccharomyces pombe. Int J Biochem. 1985;17(7):843–846. doi: 10.1016/0020-711x(85)90275-7. [DOI] [PubMed] [Google Scholar]
  12. Price N. C., Stevens E. Distinction between cofactor-dependent and -independent phosphoglycerate mutases by chromatography on Cibacron Blue-Sepharose. Biosci Rep. 1983 Sep;3(9):857–861. doi: 10.1007/BF01133784. [DOI] [PubMed] [Google Scholar]
  13. Russell G. A., Dunbar B., Fothergill-Gilmore L. A. The complete amino acid sequence of chicken skeletal-muscle enolase. Biochem J. 1986 May 15;236(1):115–126. doi: 10.1042/bj2360115. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Sanger F., Nicklen S., Coulson A. R. DNA sequencing with chain-terminating inhibitors. Proc Natl Acad Sci U S A. 1977 Dec;74(12):5463–5467. doi: 10.1073/pnas.74.12.5463. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Sedmak J. J., Grossberg S. E. A rapid, sensitive, and versatile assay for protein using Coomassie brilliant blue G250. Anal Biochem. 1977 May 1;79(1-2):544–552. doi: 10.1016/0003-2697(77)90428-6. [DOI] [PubMed] [Google Scholar]
  16. Shanske S., Sakoda S., Hermodson M. A., DiMauro S., Schon E. A. Isolation of a cDNA encoding the muscle-specific subunit of human phosphoglycerate mutase. J Biol Chem. 1987 Oct 25;262(30):14612–14617. [PubMed] [Google Scholar]
  17. Southern E. M. Detection of specific sequences among DNA fragments separated by gel electrophoresis. J Mol Biol. 1975 Nov 5;98(3):503–517. doi: 10.1016/s0022-2836(75)80083-0. [DOI] [PubMed] [Google Scholar]
  18. Stirling C. J., Szatmari G., Stewart G., Smith M. C., Sherratt D. J. The arginine repressor is essential for plasmid-stabilizing site-specific recombination at the ColE1 cer locus. EMBO J. 1988 Dec 20;7(13):4389–4395. doi: 10.1002/j.1460-2075.1988.tb03338.x. [DOI] [PMC free article] [PubMed] [Google Scholar]
  19. White M. F., Fothergill-Gilmore L. A. Sequence of the gene encoding phosphoglycerate mutase from Saccharomyces cerevisiae. FEBS Lett. 1988 Mar 14;229(2):383–387. doi: 10.1016/0014-5793(88)81161-x. [DOI] [PubMed] [Google Scholar]
  20. White P. J., Young J., Hunter I. S., Nimmo H. G., Coggins J. R. The purification and characterization of 3-dehydroquinase from Streptomyces coelicolor. Biochem J. 1990 Feb 1;265(3):735–738. doi: 10.1042/bj2650735. [DOI] [PMC free article] [PubMed] [Google Scholar]
  21. Winn S. I., Watson H. C., Harkins R. N., Fothergill L. A. Structure and activity of phosphoglycerate mutase. Philos Trans R Soc Lond B Biol Sci. 1981 Jun 26;293(1063):121–130. doi: 10.1098/rstb.1981.0066. [DOI] [PubMed] [Google Scholar]
  22. Yanagawa S., Hitomi K., Sasaki R., Chiba H. Isolation and characterization of cDNA encoding rabbit reticulocyte 2,3-bisphosphoglycerate synthase. Gene. 1986;44(2-3):185–191. doi: 10.1016/0378-1119(86)90181-2. [DOI] [PubMed] [Google Scholar]

Articles from Journal of Bacteriology are provided here courtesy of American Society for Microbiology (ASM)

RESOURCES