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. 1992 Jan;174(2):638–642. doi: 10.1128/jb.174.2.638-642.1992

Structural organization of pBC1, a cryptic plasmid from Bacillus coagulans.

E De Rossi 1, A Milano 1, P Brigidi 1, F Bini 1, G Riccardi 1
PMCID: PMC205762  PMID: 1729254

Abstract

The complete nucleotide sequence of the Bacillus coagulans plasmid pBC1 was determined. The sequence revealed an open reading frame encoding a polypeptide of 259 amino acids. This open reading frame shows sequence similarity to genes coding for replication-associated proteins in a group of gram-positive bacterial plasmids known to replicate via single-stranded intermediates. A region required for replication in cis, when the intact replicon is supplied in trans, was identified as well.

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