Skip to main content
PMC home page
Journal of Bacteriology logoLink to Journal of Bacteriology
. 1992 Apr;174(7):2388–2390. doi: 10.1128/jb.174.7.2388-2390.1992

Chromosome Partitioning in Escherichia coli in the Absence of Dam-Directed Methylation

Daniel Vinella 1, Aline Jaffé 1, Richard D'ari 1, Masamichi Kohiyama 1, Patrick Hughes 1,*
PMCID: PMC205863  PMID: 1551854

Abstract

Escherichia coli dam mutants, lacking the GATC DNA methylase, do not produce anucleate cells at high frequencies, suggesting that hemimethylation of the chromosome origin of replication, oriC, is not essential for correct chromosome partitioning.

Full text

PDF
2389

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Bakker A., Smith D. W. Methylation of GATC sites is required for precise timing between rounds of DNA replication in Escherichia coli. J Bacteriol. 1989 Oct;171(10):5738–5742. doi: 10.1128/jb.171.10.5738-5742.1989. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Boye E., Løbner-Olesen A. The role of dam methyltransferase in the control of DNA replication in E. coli. Cell. 1990 Sep 7;62(5):981–989. doi: 10.1016/0092-8674(90)90272-g. [DOI] [PubMed] [Google Scholar]
  3. Campbell J. L., Kleckner N. E. coli oriC and the dnaA gene promoter are sequestered from dam methyltransferase following the passage of the chromosomal replication fork. Cell. 1990 Sep 7;62(5):967–979. doi: 10.1016/0092-8674(90)90271-f. [DOI] [PubMed] [Google Scholar]
  4. D'Ari R., Huisman O. Novel mechanism of cell division inhibition associated with the SOS response in Escherichia coli. J Bacteriol. 1983 Oct;156(1):243–250. doi: 10.1128/jb.156.1.243-250.1983. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Glickman B. W., Radman M. Escherichia coli mutator mutants deficient in methylation-instructed DNA mismatch correction. Proc Natl Acad Sci U S A. 1980 Feb;77(2):1063–1067. doi: 10.1073/pnas.77.2.1063. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Hendrickson W. G., Kusano T., Yamaki H., Balakrishnan R., King M., Murchie J., Schaechter M. Binding of the origin of replication of Escherichia coli to the outer membrane. Cell. 1982 Oct;30(3):915–923. doi: 10.1016/0092-8674(82)90296-3. [DOI] [PubMed] [Google Scholar]
  7. Hiraga S., Niki H., Imamura R., Ogura T., Yamanaka K., Feng J., Ezaki B., Jaffé A. Mutants defective in chromosome partitioning in E. coli. Res Microbiol. 1991 Feb-Apr;142(2-3):189–194. doi: 10.1016/0923-2508(91)90029-a. [DOI] [PubMed] [Google Scholar]
  8. Hiraga S., Niki H., Ogura T., Ichinose C., Mori H., Ezaki B., Jaffé A. Chromosome partitioning in Escherichia coli: novel mutants producing anucleate cells. J Bacteriol. 1989 Mar;171(3):1496–1505. doi: 10.1128/jb.171.3.1496-1505.1989. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Hussain K., Elliott E. J., Salmond G. P. The parD- mutant of Escherichia coli also carries a gyrAam mutation. The complete sequence of gyrA. Mol Microbiol. 1987 Nov;1(3):259–273. doi: 10.1111/j.1365-2958.1987.tb01932.x. [DOI] [PubMed] [Google Scholar]
  10. Jaffé A., D'Ari R., Norris V. SOS-independent coupling between DNA replication and cell division in Escherichia coli. J Bacteriol. 1986 Jan;165(1):66–71. doi: 10.1128/jb.165.1.66-71.1986. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Kato J., Nishimura Y., Imamura R., Niki H., Hiraga S., Suzuki H. New topoisomerase essential for chromosome segregation in E. coli. Cell. 1990 Oct 19;63(2):393–404. doi: 10.1016/0092-8674(90)90172-b. [DOI] [PubMed] [Google Scholar]
  12. Landoulsi A., Malki A., Kern R., Kohiyama M., Hughes P. The E. coli cell surface specifically prevents the initiation of DNA replication at oriC on hemimethylated DNA templates. Cell. 1990 Nov 30;63(5):1053–1060. doi: 10.1016/0092-8674(90)90508-c. [DOI] [PubMed] [Google Scholar]
  13. Leonard A. C., Helmstetter C. E. Cell cycle-specific replication of Escherichia coli minichromosomes. Proc Natl Acad Sci U S A. 1986 Jul;83(14):5101–5105. doi: 10.1073/pnas.83.14.5101. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Marinus M. G., Carraway M., Frey A. Z., Brown L., Arraj J. A. Insertion mutations in the dam gene of Escherichia coli K-12. Mol Gen Genet. 1983;192(1-2):288–289. doi: 10.1007/BF00327681. [DOI] [PubMed] [Google Scholar]
  15. Marinus M. G. DNA methylation in Escherichia coli. Annu Rev Genet. 1987;21:113–131. doi: 10.1146/annurev.ge.21.120187.000553. [DOI] [PubMed] [Google Scholar]
  16. Niki H., Jaffé A., Imamura R., Ogura T., Hiraga S. The new gene mukB codes for a 177 kd protein with coiled-coil domains involved in chromosome partitioning of E. coli. EMBO J. 1991 Jan;10(1):183–193. doi: 10.1002/j.1460-2075.1991.tb07935.x. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Norris V., Alliotte T., Jaffé A., D'Ari R. DNA replication termination in Escherichia coli parB (a dnaG allele), parA, and gyrB mutants affected in DNA distribution. J Bacteriol. 1986 Nov;168(2):494–504. doi: 10.1128/jb.168.2.494-504.1986. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. Ogden G. B., Pratt M. J., Schaechter M. The replicative origin of the E. coli chromosome binds to cell membranes only when hemimethylated. Cell. 1988 Jul 1;54(1):127–135. doi: 10.1016/0092-8674(88)90186-9. [DOI] [PubMed] [Google Scholar]
  19. Ogura T., Miki T., Hiraga S. Copy-number mutants of the plasmid carrying the replication origin of the Escherichia coli chromosome: evidence for a control region of replication. Proc Natl Acad Sci U S A. 1980 Jul;77(7):3993–3997. doi: 10.1073/pnas.77.7.3993. [DOI] [PMC free article] [PubMed] [Google Scholar]
  20. Pang P. P., Lundberg A. S., Walker G. C. Identification and characterization of the mutL and mutS gene products of Salmonella typhimurium LT2. J Bacteriol. 1985 Sep;163(3):1007–1015. doi: 10.1128/jb.163.3.1007-1015.1985. [DOI] [PMC free article] [PubMed] [Google Scholar]
  21. Peterson K. R., Wertman K. F., Mount D. W., Marinus M. G. Viability of Escherichia coli K-12 DNA adenine methylase (dam) mutants requires increased expression of specific genes in the SOS regulon. Mol Gen Genet. 1985;201(1):14–19. doi: 10.1007/BF00397979. [DOI] [PubMed] [Google Scholar]
  22. Russell D. W., Zinder N. D. Hemimethylation prevents DNA replication in E. coli. Cell. 1987 Sep 25;50(7):1071–1079. doi: 10.1016/0092-8674(87)90173-5. [DOI] [PubMed] [Google Scholar]
  23. Sekimizu K., Yung B. Y., Kornberg A. The dnaA protein of Escherichia coli. Abundance, improved purification, and membrane binding. J Biol Chem. 1988 May 25;263(15):7136–7140. [PubMed] [Google Scholar]
  24. Shpetner H. S., Vallee R. B. Identification of dynamin, a novel mechanochemical enzyme that mediates interactions between microtubules. Cell. 1989 Nov 3;59(3):421–432. doi: 10.1016/0092-8674(89)90027-5. [DOI] [PubMed] [Google Scholar]
  25. de Boer P. A., Crossley R. E., Rothfield L. I. A division inhibitor and a topological specificity factor coded for by the minicell locus determine proper placement of the division septum in E. coli. Cell. 1989 Feb 24;56(4):641–649. doi: 10.1016/0092-8674(89)90586-2. [DOI] [PubMed] [Google Scholar]

Articles from Journal of Bacteriology are provided here courtesy of American Society for Microbiology (ASM)

RESOURCES