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. 1992 Jun;174(11):3494–3498. doi: 10.1128/jb.174.11.3494-3498.1992

Physical map of Campylobacter jejuni TGH9011 and localization of 10 genetic markers by use of pulsed-field gel electrophoresis.

N W Kim 1, H Bingham 1, R Khawaja 1, H Louie 1, E Hani 1, K Neote 1, V L Chan 1
PMCID: PMC206033  PMID: 1350583

Abstract

The physical map of Campylobacter jejuni TGH9011 (ATCC 43430) was constructed by mapping the three restriction enzyme sites SacII (CCGCGG), SalI (GTCGAC), and SmaI (CCCGGG) on the genome of C. jejuni by using pulsed-field gel electrophoresis and Southern hybridization. A total of 25 restriction enzyme sites were mapped onto the C. jejuni chromosome. The size of the genome was reevaluated and was shown to be 1,812.5 kb. Ten C. jejuni genetic markers that have been isolated in our laboratory were mapped to specific restriction enzyme fragments. Furthermore, we have accurately mapped one of the three rRNA operons (rrnA) and have demonstrated a separation of the 16S and 23S rRNA-encoding sequences in one of the rRNA operons.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Allardet-Servent A., Carles-Nurit M. J., Bourg G., Michaux S., Ramuz M. Physical map of the Brucella melitensis 16 M chromosome. J Bacteriol. 1991 Apr;173(7):2219–2224. doi: 10.1128/jb.173.7.2219-2224.1991. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Carle G. F., Olson M. V. An electrophoretic karyotype for yeast. Proc Natl Acad Sci U S A. 1985 Jun;82(11):3756–3760. doi: 10.1073/pnas.82.11.3756. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Chan V. L., Bingham H. L. Complete sequence of the Campylobacter jejuni glyA gene encoding serine hydroxymethyltransferase. Gene. 1991 May 15;101(1):51–58. doi: 10.1016/0378-1119(91)90223-x. [DOI] [PubMed] [Google Scholar]
  4. Chan V. L., Bingham H. L. Lysyl-tRNA synthetase gene of Campylobacter jejuni. J Bacteriol. 1992 Feb;174(3):695–701. doi: 10.1128/jb.174.3.695-701.1992. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Chan V. L., Bingham H., Kibue A., Nayudu P. R., Penner J. L. Cloning and expression of the Campylobacter jejuni glyA gene in Escherichia coli. Gene. 1988 Dec 15;73(1):185–191. doi: 10.1016/0378-1119(88)90324-1. [DOI] [PubMed] [Google Scholar]
  6. Chang N., Taylor D. E. Use of pulsed-field agarose gel electrophoresis to size genomes of Campylobacter species and to construct a SalI map of Campylobacter jejuni UA580. J Bacteriol. 1990 Sep;172(9):5211–5217. doi: 10.1128/jb.172.9.5211-5217.1990. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Chen X., Finch L. R. Novel arrangement of rRNA genes in Mycoplasma gallisepticum: separation of the 16S gene of one set from the 23S and 5S genes. J Bacteriol. 1989 May;171(5):2876–2878. doi: 10.1128/jb.171.5.2876-2878.1989. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Hartmann R. K., Ulbrich N., Erdmann V. A. An unusual rRNA operon constellation: in Thermus thermophilus HB8 the 23S/5S rRNA operon is a separate entity from the 16S rRNA operon. Biochimie. 1987 Oct;69(10):1097–1104. doi: 10.1016/0300-9084(87)90009-5. [DOI] [PubMed] [Google Scholar]
  9. Johnson W. M., Lior H. Cytotoxic and cytotonic factors produced by Campylobacter jejuni, Campylobacter coli, and Campylobacter laridis. J Clin Microbiol. 1986 Aug;24(2):275–281. doi: 10.1128/jcm.24.2.275-281.1986. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Lee J. J., Smith H. O. Sizing of the Haemophilus influenzae Rd genome by pulsed-field agarose gel electrophoresis. J Bacteriol. 1988 Sep;170(9):4402–4405. doi: 10.1128/jb.170.9.4402-4405.1988. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Nuijten P. J., Bartels C., Bleumink-Pluym N. M., Gaastra W., van der Zeijst B. A. Size and physical map of the Campylobacter jejuni chromosome. Nucleic Acids Res. 1990 Nov 11;18(21):6211–6214. doi: 10.1093/nar/18.21.6211. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Owen R. J. Nucleic acids in the classification of Campylobacters. Eur J Clin Microbiol. 1983 Aug;2(4):367–377. doi: 10.1007/BF02019473. [DOI] [PubMed] [Google Scholar]
  13. Pace N. R. Structure and synthesis of the ribosomal ribonucleic acid of prokaryotes. Bacteriol Rev. 1973 Dec;37(4):562–603. doi: 10.1128/br.37.4.562-603.1973. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Römling U., Grothues D., Bautsch W., Tümmler B. A physical genome map of Pseudomonas aeruginosa PAO. EMBO J. 1989 Dec 20;8(13):4081–4089. doi: 10.1002/j.1460-2075.1989.tb08592.x. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Römling U., Tümmler B. The impact of two-dimensional pulsed-field gel electrophoresis techniques for the consistent and complete mapping of bacterial genomes: refined physical map of Pseudomonas aeruginosa PAO. Nucleic Acids Res. 1991 Jun 25;19(12):3199–3206. doi: 10.1093/nar/19.12.3199. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Smith C. L., Econome J. G., Schutt A., Klco S., Cantor C. R. A physical map of the Escherichia coli K12 genome. Science. 1987 Jun 12;236(4807):1448–1453. doi: 10.1126/science.3296194. [DOI] [PubMed] [Google Scholar]
  17. Vollrath D., Davis R. W., Connelly C., Hieter P. Physical mapping of large DNA by chromosome fragmentation. Proc Natl Acad Sci U S A. 1988 Aug;85(16):6027–6031. doi: 10.1073/pnas.85.16.6027. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. Vollrath D., Davis R. W. Resolution of DNA molecules greater than 5 megabases by contour-clamped homogeneous electric fields. Nucleic Acids Res. 1987 Oct 12;15(19):7865–7876. doi: 10.1093/nar/15.19.7865. [DOI] [PMC free article] [PubMed] [Google Scholar]
  19. Walker R. I., Caldwell M. B., Lee E. C., Guerry P., Trust T. J., Ruiz-Palacios G. M. Pathophysiology of Campylobacter enteritis. Microbiol Rev. 1986 Mar;50(1):81–94. doi: 10.1128/mr.50.1.81-94.1986. [DOI] [PMC free article] [PubMed] [Google Scholar]
  20. Wang Y., Taylor D. E. Natural transformation in Campylobacter species. J Bacteriol. 1990 Feb;172(2):949–955. doi: 10.1128/jb.172.2.949-955.1990. [DOI] [PMC free article] [PubMed] [Google Scholar]
  21. Yan W., Taylor D. E. Sizing and mapping of the genome of Campylobacter coli strain UA417R using pulsed-field gel electrophoresis. Gene. 1991 May 15;101(1):117–120. doi: 10.1016/0378-1119(91)90232-z. [DOI] [PubMed] [Google Scholar]

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