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. 1992 Feb;174(3):1050–1052. doi: 10.1128/jb.174.3.1050-1052.1992

Biosynthesis of vitamin B12 in Pseudomonas denitrificans: the biosynthetic sequence from precorrin-6y to precorrin-8x is catalyzed by the cobL gene product.

F Blanche 1, A Famechon 1, D Thibaut 1, L Debussche 1, B Cameron 1, J Crouzet 1
PMCID: PMC206191  PMID: 1732195

Abstract

A protein catalyzing methylation at C-5 and C-15 and decarboxylation of the acetic acid side chain at C-12 on precorrin-6y to yield precorrin-8x was purified to homogeneity from a recombinant strain of Pseudomonas denitrificans. It was sequenced at the N terminus and shown to be encoded by the cobL gene.

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Selected References

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