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. 1997;75(5):678–683. doi: 10.1038/bjc.1997.121

A follow-up study of progression from dysplasia to squamous cell carcinoma with immunohistochemical examination of p53 protein overexpression in the bronchi of ex-chromate workers.

Y Satoh 1, Y Ishikawa 1, K Nakagawa 1, T Hirano 1, E Tsuchiya 1
PMCID: PMC2063337  PMID: 9043024

Abstract

Squamous cell carcinoma (SCC) of the bronchus is considered to develop from preneoplastic 'dysplasia', but reports of sequential observation of this dysplasia-carcinoma sequence in humans are very few. We followed four dysplastic lesions found in the bronchi of three ex-chromate workers by bronchoscopy and biopsy and found that all of them progressed to SCC. Of the four lesions, three were severe dysplasias at the first biopsy which progressed to SCCs in 7-13 months. The last one was a slight dysplasia at the first biopsy and showed progression of the atypia to carcinoma in 6 years and 10 months. An immunohistochemical analysis of the chronological change in p53 protein expression in these lesions and in normal ciliated epithelium taken from the surroundings was conducted in each case. Overexpression of p53 protein was observed in two of the severe dysplasias and the one slight dysplasia, as well as their eventual SCCs. However, no such change was apparent in one case of severe dysplasia or its eventual SCC. Normal epithelium was consistently negative. Our results provide direct proof of the dysplasia-carcinoma sequence and suggest that alteration in the expression of p53 protein might be an important early event which persists. Therefore, the immunohistochemical detection of p53 overexpression in biopsy specimens of bronchial epithelium might be useful for evaluation of preneoplastic lesions in high-risk group individuals and for early diagnosis of bronchial cancer.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. BAETJER A. M. Pulmonary carcinoma in chromate workers. 1. A review of the literature and report of cases. AMA Arch Ind Hyg Occup Med. 1950 Nov;2(5):487–504. [PubMed] [Google Scholar]
  2. Bennett W. P., Colby T. V., Travis W. D., Borkowski A., Jones R. T., Lane D. P., Metcalf R. A., Samet J. M., Takeshima Y., Gu J. R. p53 protein accumulates frequently in early bronchial neoplasia. Cancer Res. 1993 Oct 15;53(20):4817–4822. [PubMed] [Google Scholar]
  3. Greenblatt M. S., Bennett W. P., Hollstein M., Harris C. C. Mutations in the p53 tumor suppressor gene: clues to cancer etiology and molecular pathogenesis. Cancer Res. 1994 Sep 15;54(18):4855–4878. [PubMed] [Google Scholar]
  4. Ishikawa Y., Nakagawa K., Satoh Y., Kitagawa T., Sugano H., Hirano T., Tsuchiya E. Characteristics of chromate workers' cancers, chromium lung deposition and precancerous bronchial lesions: an autopsy study. Br J Cancer. 1994 Jul;70(1):160–166. doi: 10.1038/bjc.1994.268. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Klein N., Vignaud J. M., Sadmi M., Plenat F., Borelly J., Duprez A., Martinet Y., Martinet N. Squamous metaplasia expression of proto-oncogenes and P 53 in lung cancer patients. Lab Invest. 1993 Jan;68(1):26–32. [PubMed] [Google Scholar]
  6. Nuorva K., Soini Y., Kamel D., Autio-Harmainen H., Risteli L., Risteli J., Vähäkangas K., Päkkö P. Concurrent p53 expression in bronchial dysplasias and squamous cell lung carcinomas. Am J Pathol. 1993 Mar;142(3):725–732. [PMC free article] [PubMed] [Google Scholar]
  7. Saccomanno G., Archer V. E., Auerbach O., Saunders R. P., Brennan L. M. Development of carcinoma of the lung as reflected in exfoliated cells. Cancer. 1974 Jan;33(1):256–270. doi: 10.1002/1097-0142(197401)33:1<256::aid-cncr2820330139>3.0.co;2-g. [DOI] [PubMed] [Google Scholar]
  8. Sozzi G., Miozzo M., Donghi R., Pilotti S., Cariani C. T., Pastorino U., Della Porta G., Pierotti M. A. Deletions of 17p and p53 mutations in preneoplastic lesions of the lung. Cancer Res. 1992 Nov 1;52(21):6079–6082. [PubMed] [Google Scholar]
  9. Sundaresan V., Ganly P., Hasleton P., Rudd R., Sinha G., Bleehen N. M., Rabbitts P. p53 and chromosome 3 abnormalities, characteristic of malignant lung tumours, are detectable in preinvasive lesions of the bronchus. Oncogene. 1992 Oct;7(10):1989–1997. [PubMed] [Google Scholar]
  10. TIPTON D. L., CROCKER T. T. DURATION OF BRONCHIAL SQUAMOUS METAPLASIA PRODUCED IN DOGS BY CIGARETTE SMOKE CONDENSATE. J Natl Cancer Inst. 1964 Sep;33:487–495. [PubMed] [Google Scholar]
  11. Thiberville L., Payne P., Vielkinds J., LeRiche J., Horsman D., Nouvet G., Palcic B., Lam S. Evidence of cumulative gene losses with progression of premalignant epithelial lesions to carcinoma of the bronchus. Cancer Res. 1995 Nov 15;55(22):5133–5139. [PubMed] [Google Scholar]
  12. Vogelstein B. Cancer. A deadly inheritance. Nature. 1990 Dec 20;348(6303):681–682. doi: 10.1038/348681a0. [DOI] [PubMed] [Google Scholar]

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