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. 1992 Aug;174(16):5251–5257. doi: 10.1128/jb.174.16.5251-5257.1992

Linear- and circular-plasmid copy numbers in Borrelia burgdorferi.

J Hinnebusch 1, A G Barbour 1
PMCID: PMC206359  PMID: 1644750

Abstract

Borrelia burgdorferi, the Lyme disease agent, and other members of the spirochetal genus Borrelia have double-stranded linear plasmids in addition to supercoiled circular plasmids. The copy number relative to the chromosome was determined for 49- and 16-kb linear plasmids and a 27-kb circular plasmid of the type strain, B31, of B. burgdorferi. All three plasmids were present in low copy number, about one per chromosome equivalent, as determined by relative hybridizations of replicon-specific DNA probes. The low copy number of Borrelia plasmids suggests that initiation of DNA replication and partitioning are carefully controlled during the cell division cycle. The copy numbers of these three plasmids of strain B31 were unchanged after approximately 7,000 generations in continuous in vitro culture. A clone of B. burgdorferi B31 that did not contain the 16-kb linear plasmid was obtained after exposure of a culture to novobiocin, a DNA gyrase inhibitor. The plasmid-cured strain contains only one linear plasmid, the 49-kb plasmid, and thus has the smallest genome reported to date for B. burgdorferi.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Austin S., Nordström K. Partition-mediated incompatibility of bacterial plasmids. Cell. 1990 Feb 9;60(3):351–354. doi: 10.1016/0092-8674(90)90584-2. [DOI] [PubMed] [Google Scholar]
  2. Barbour A. G., Garon C. F. Linear plasmids of the bacterium Borrelia burgdorferi have covalently closed ends. Science. 1987 Jul 24;237(4813):409–411. doi: 10.1126/science.3603026. [DOI] [PubMed] [Google Scholar]
  3. Barbour A. G., Garon C. F. The genes encoding major surface proteins of Borrelia burgdorferi are located on a plasmid. Ann N Y Acad Sci. 1988;539:144–153. doi: 10.1111/j.1749-6632.1988.tb31847.x. [DOI] [PubMed] [Google Scholar]
  4. Barbour A. G., Hayes S. F. Biology of Borrelia species. Microbiol Rev. 1986 Dec;50(4):381–400. doi: 10.1128/mr.50.4.381-400.1986. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Barbour A. G. Isolation and cultivation of Lyme disease spirochetes. Yale J Biol Med. 1984 Jul-Aug;57(4):521–525. [PMC free article] [PubMed] [Google Scholar]
  6. Barbour A. G. Plasmid analysis of Borrelia burgdorferi, the Lyme disease agent. J Clin Microbiol. 1988 Mar;26(3):475–478. doi: 10.1128/jcm.26.3.475-478.1988. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Baril C., Richaud C., Baranton G., Saint Girons I. S. Linear chromosome of Borrelia burgdorferi. Res Microbiol. 1989 Oct;140(8):507–516. doi: 10.1016/0923-2508(89)90083-1. [DOI] [PubMed] [Google Scholar]
  8. Bergström S., Bundoc V. G., Barbour A. G. Molecular analysis of linear plasmid-encoded major surface proteins, OspA and OspB, of the Lyme disease spirochaete Borrelia burgdorferi. Mol Microbiol. 1989 Apr;3(4):479–486. doi: 10.1111/j.1365-2958.1989.tb00194.x. [DOI] [PubMed] [Google Scholar]
  9. Bresser J., Doering J., Gillespie D. Quick-blot: selective mRNA or DNA immobilization from whole cells. DNA. 1983;2(3):243–254. doi: 10.1089/dna.1983.2.243. [DOI] [PubMed] [Google Scholar]
  10. Bundoc V. G., Barbour A. G. Clonal polymorphisms of outer membrane protein OspB of Borrelia burgdorferi. Infect Immun. 1989 Sep;57(9):2733–2741. doi: 10.1128/iai.57.9.2733-2741.1989. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Burgdorfer W., Barbour A. G., Hayes S. F., Benach J. L., Grunwaldt E., Davis J. P. Lyme disease-a tick-borne spirochetosis? Science. 1982 Jun 18;216(4552):1317–1319. doi: 10.1126/science.7043737. [DOI] [PubMed] [Google Scholar]
  12. Costanzi C., Gillespie D. Fast blots: immobilization of DNA and RNA from cells. Methods Enzymol. 1987;152:582–587. doi: 10.1016/0076-6879(87)52065-1. [DOI] [PubMed] [Google Scholar]
  13. Cozzarelli N. R. The mechanism of action of inhibitors of DNA synthesis. Annu Rev Biochem. 1977;46:641–668. doi: 10.1146/annurev.bi.46.070177.003233. [DOI] [PubMed] [Google Scholar]
  14. Ferdows M. S., Barbour A. G. Megabase-sized linear DNA in the bacterium Borrelia burgdorferi, the Lyme disease agent. Proc Natl Acad Sci U S A. 1989 Aug;86(15):5969–5973. doi: 10.1073/pnas.86.15.5969. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Garon C. F., Dorward D. W., Corwin M. D. Structural features of Borrelia burgdorferi--the Lyme disease spirochete: silver staining for nucleic acids. Scanning Microsc Suppl. 1989;3:109–115. [PubMed] [Google Scholar]
  16. Hanahan D. Studies on transformation of Escherichia coli with plasmids. J Mol Biol. 1983 Jun 5;166(4):557–580. doi: 10.1016/s0022-2836(83)80284-8. [DOI] [PubMed] [Google Scholar]
  17. Hayes L. J., Wright D. J., Archard L. C. Segmented arrangement of Borrelia duttonii DNA and location of variant surface antigen genes. J Gen Microbiol. 1988 Jul;134(7):1785–1793. doi: 10.1099/00221287-134-7-1785. [DOI] [PubMed] [Google Scholar]
  18. Hinnebusch J., Barbour A. G. Linear plasmids of Borrelia burgdorferi have a telomeric structure and sequence similar to those of a eukaryotic virus. J Bacteriol. 1991 Nov;173(22):7233–7239. doi: 10.1128/jb.173.22.7233-7239.1991. [DOI] [PMC free article] [PubMed] [Google Scholar]
  19. Hinnebusch J., Bergström S., Barbour A. G. Cloning and sequence analysis of linear plasmid telomeres of the bacterium Borrelia burgdorferi. Mol Microbiol. 1990 May;4(5):811–820. doi: 10.1111/j.1365-2958.1990.tb00651.x. [DOI] [PubMed] [Google Scholar]
  20. Howe T. R., LaQuier F. W., Barbour A. G. Organization of genes encoding two outer membrane proteins of the Lyme disease agent Borrelia burgdorferi within a single transcriptional unit. Infect Immun. 1986 Oct;54(1):207–212. doi: 10.1128/iai.54.1.207-212.1986. [DOI] [PMC free article] [PubMed] [Google Scholar]
  21. Howe T. R., Mayer L. W., Barbour A. G. A single recombinant plasmid expressing two major outer surface proteins of the Lyme disease spirochete. Science. 1985 Feb 8;227(4687):645–646. doi: 10.1126/science.3969554. [DOI] [PubMed] [Google Scholar]
  22. Hyde F. W., Johnson R. C. Characterization of a circular plasmid from Borrelia burgdorferi, etiologic agent of Lyme disease. J Clin Microbiol. 1988 Oct;26(10):2203–2205. doi: 10.1128/jcm.26.10.2203-2205.1988. [DOI] [PMC free article] [PubMed] [Google Scholar]
  23. Hyde F. W., Johnson R. C. Genetic relationship of lyme disease spirochetes to Borrelia, Treponema, and Leptospira spp. J Clin Microbiol. 1984 Aug;20(2):151–154. doi: 10.1128/jcm.20.2.151-154.1984. [DOI] [PMC free article] [PubMed] [Google Scholar]
  24. Kurtti T. J., Munderloh U. G., Johnson R. C., Ahlstrand G. G. Colony formation and morphology in Borrelia burgdorferi. J Clin Microbiol. 1987 Nov;25(11):2054–2058. doi: 10.1128/jcm.25.11.2054-2058.1987. [DOI] [PMC free article] [PubMed] [Google Scholar]
  25. McHugh G. L., Swartz M. N. Elimination of plasmids from several bacterial species by novobiocin. Antimicrob Agents Chemother. 1977 Sep;12(3):423–426. doi: 10.1128/aac.12.3.423. [DOI] [PMC free article] [PubMed] [Google Scholar]
  26. Meier J. T., Simon M. I., Barbour A. G. Antigenic variation is associated with DNA rearrangements in a relapsing fever Borrelia. Cell. 1985 Jun;41(2):403–409. doi: 10.1016/s0092-8674(85)80013-1. [DOI] [PubMed] [Google Scholar]
  27. Nordström K., Austin S. J. Mechanisms that contribute to the stable segregation of plasmids. Annu Rev Genet. 1989;23:37–69. doi: 10.1146/annurev.ge.23.120189.000345. [DOI] [PubMed] [Google Scholar]
  28. Plasterk R. H., Simon M. I., Barbour A. G. Transposition of structural genes to an expression sequence on a linear plasmid causes antigenic variation in the bacterium Borrelia hermsii. Nature. 1985 Nov 21;318(6043):257–263. doi: 10.1038/318257a0. [DOI] [PubMed] [Google Scholar]
  29. Schwan T. G., Burgdorfer W., Garon C. F. Changes in infectivity and plasmid profile of the Lyme disease spirochete, Borrelia burgdorferi, as a result of in vitro cultivation. Infect Immun. 1988 Aug;56(8):1831–1836. doi: 10.1128/iai.56.8.1831-1836.1988. [DOI] [PMC free article] [PubMed] [Google Scholar]
  30. Simpson W. J., Garon C. F., Schwan T. G. Analysis of supercoiled circular plasmids in infectious and non-infectious Borrelia burgdorferi. Microb Pathog. 1990 Feb;8(2):109–118. doi: 10.1016/0882-4010(90)90075-2. [DOI] [PubMed] [Google Scholar]
  31. Stålhammar-Carlemalm M., Jenny E., Gern L., Aeschlimann A., Meyer J. Plasmid analysis and restriction fragment length polymorphisms of chromosomal DNA allow a distinction between Borrelia burgdorferi strains. Zentralbl Bakteriol. 1990 Oct;274(1):28–39. doi: 10.1016/s0934-8840(11)80972-2. [DOI] [PubMed] [Google Scholar]

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