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. 1993 Sep;175(17):5324–5328. doi: 10.1128/jb.175.17.5324-5328.1993

Escherichia coli produces linoleic acid during late stationary phase.

H D Rabinowitch 1, D Sklan 1, D H Chace 1, R D Stevens 1, I Fridovich 1
PMCID: PMC206585  PMID: 8366020

Abstract

Escherichia coli produces linoleic acid in the late stationary phase. This was the case whether the cultures were grown aerobically or anaerobically on a supplemented glucose-salts medium. The linoleic acid was detected by thin-layer chromatography and was measured as the methyl ester by gas chromatography. The linoleic acid methyl ester was identified by its mass spectrum. Lipids extracted from late-stationary-phase cells generated thiobarbituric acid-reactive carbonyl products when incubated with a free radical initiator. In contrast, extracts from log-phase or early-stationary-phase cells failed to do so, in accordance with the presence of polyunsaturated fatty acid only in the stationary-phase cells.

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Selected References

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