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. 1993 Sep;175(17):5697–5700. doi: 10.1128/jb.175.17.5697-5700.1993

Chloramphenicol acetyltransferase, a cytoplasmic protein is incompatible for export from Bacillus subtilis.

M W Chen 1, V Nagarajan 1
PMCID: PMC206629  PMID: 8366055

Abstract

Bacillus subtilis cells expressing a hybrid protein (Lvsss-Cat) consisting of the B. amyloliquefaciens levansucrase signal peptide fused to B. pumilus chloramphenicol acetyltransferase (Cat) are unable to export Cat protein into the growth medium. A series of tripartite protein fusions was constructed by inserting various lengths of the Cat sequences between the levansucrase signal peptide and staphylococcal protein A or Escherichia coli alkaline phosphatase. Biochemical characterization of the various Cat protein fusions revealed that multiple regions in the Cat protein were causing the export defect.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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