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. 1993 Sep;175(18):5824–5828. doi: 10.1128/jb.175.18.5824-5828.1993

Chloramphenicol induces the transcription of the major cold shock gene of Escherichia coli, cspA.

W Jiang 1, P Jones 1, M Inouye 1
PMCID: PMC206661  PMID: 8376329

Abstract

A downshift in temperature or exposure of cells to certain inhibitors of translation has been shown to induce the synthesis of cold shock proteins in Escherichia coli. We characterized the induction of the major cold shock protein (CS7.4, the product of the cspA gene) of E. coli in response to a shift from 37 to 15 degrees C and in response to the addition of chloramphenicol at 15 degrees C. A pulse-labeling assay and primer extension experiments indicated that the cold shock treatment resulted in a transient increase in the level of the cspA transcript and a transient induction of CS7.4, while the addition of chloramphenicol resulted in a constitutive increase in the level of cspA transcript and constitutive production of CS7.4. The addition of rifamycin immediately following the temperature downshift or along with the addition of chloramphenicol repressed the transcription of cspA as well as the induced production of CS7.4. Furthermore, changes in the cspA mRNA level were coincident with changes in CS7.4 synthesis. These results indicate that the expression of cspA induced by cold shock and chloramphenicol is at the level of transcription but not at the level of translation. Measurement of the half-life revealed that the cspA mRNA induced by chloramphenicol was more stable than that induced by cold shock.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Av-Gay Y., Aharonowitz Y., Cohen G. Streptomyces contain a 7.0 kDa cold shock like protein. Nucleic Acids Res. 1992 Oct 25;20(20):5478–5478. doi: 10.1093/nar/20.20.5478. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Goldstein J., Pollitt N. S., Inouye M. Major cold shock protein of Escherichia coli. Proc Natl Acad Sci U S A. 1990 Jan;87(1):283–287. doi: 10.1073/pnas.87.1.283. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Inouye S., Soberon X., Franceschini T., Nakamura K., Itakura K., Inouye M. Role of positive charge on the amino-terminal region of the signal peptide in protein secretion across the membrane. Proc Natl Acad Sci U S A. 1982 Jun;79(11):3438–3441. doi: 10.1073/pnas.79.11.3438. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Jones P. G., Cashel M., Glaser G., Neidhardt F. C. Function of a relaxed-like state following temperature downshifts in Escherichia coli. J Bacteriol. 1992 Jun;174(12):3903–3914. doi: 10.1128/jb.174.12.3903-3914.1992. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Jones P. G., Krah R., Tafuri S. R., Wolffe A. P. DNA gyrase, CS7.4, and the cold shock response in Escherichia coli. J Bacteriol. 1992 Sep;174(18):5798–5802. doi: 10.1128/jb.174.18.5798-5802.1992. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Jones P. G., VanBogelen R. A., Neidhardt F. C. Induction of proteins in response to low temperature in Escherichia coli. J Bacteriol. 1987 May;169(5):2092–2095. doi: 10.1128/jb.169.5.2092-2095.1987. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. La Teana A., Brandi A., Falconi M., Spurio R., Pon C. L., Gualerzi C. O. Identification of a cold shock transcriptional enhancer of the Escherichia coli gene encoding nucleoid protein H-NS. Proc Natl Acad Sci U S A. 1991 Dec 1;88(23):10907–10911. doi: 10.1073/pnas.88.23.10907. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Lund E., Kjeldgaard N. O. Metabolism of guanosine tetraphosphate in Escherichia coli. Eur J Biochem. 1972 Jul 24;28(3):316–326. doi: 10.1111/j.1432-1033.1972.tb01916.x. [DOI] [PubMed] [Google Scholar]
  9. Mackow E. R., Chang F. N. Correlation between RNA synthesis and ppGpp content in Escherichia coli during temperature shifts. Mol Gen Genet. 1983;192(1-2):5–9. doi: 10.1007/BF00327639. [DOI] [PubMed] [Google Scholar]
  10. Nagai H., Yuzawa H., Yura T. Interplay of two cis-acting mRNA regions in translational control of sigma 32 synthesis during the heat shock response of Escherichia coli. Proc Natl Acad Sci U S A. 1991 Dec 1;88(23):10515–10519. doi: 10.1073/pnas.88.23.10515. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Nakamura K., Masui Y., Inouye M. Use of a lac promoter-operator fragment as a transcriptional control switch for expression of the constitutive lpp gene in Escherichia coli. J Mol Appl Genet. 1982;1(4):289–299. [PubMed] [Google Scholar]
  12. Pao C. C., Dyess B. T. Stringent control of RNA synthesis in the absence of guanosine 5'-diphosphate-3'-diphosphate. J Biol Chem. 1981 Mar 10;256(5):2252–2257. [PubMed] [Google Scholar]
  13. Pestka S. Inhibitors of ribosome functions. Annu Rev Microbiol. 1971;25:487–562. doi: 10.1146/annurev.mi.25.100171.002415. [DOI] [PubMed] [Google Scholar]
  14. Platt T. Transcription termination and the regulation of gene expression. Annu Rev Biochem. 1986;55:339–372. doi: 10.1146/annurev.bi.55.070186.002011. [DOI] [PubMed] [Google Scholar]
  15. Qoronfleh M. W., Debouck C., Keller J. Identification and characterization of novel low-temperature-inducible promoters of Escherichia coli. J Bacteriol. 1992 Dec;174(24):7902–7909. doi: 10.1128/jb.174.24.7902-7909.1992. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Straus D. B., Walter W. A., Gross C. A. The heat shock response of E. coli is regulated by changes in the concentration of sigma 32. Nature. 1987 Sep 24;329(6137):348–351. doi: 10.1038/329348a0. [DOI] [PubMed] [Google Scholar]
  17. Tafuri S. R., Wolffe A. P. DNA binding, multimerization, and transcription stimulation by the Xenopus Y box proteins in vitro. New Biol. 1992 Apr;4(4):349–359. [PubMed] [Google Scholar]
  18. Tafuri S. R., Wolffe A. P. Xenopus Y-box transcription factors: molecular cloning, functional analysis and developmental regulation. Proc Natl Acad Sci U S A. 1990 Nov;87(22):9028–9032. doi: 10.1073/pnas.87.22.9028. [DOI] [PMC free article] [PubMed] [Google Scholar]
  19. Tanabe H., Goldstein J., Yang M., Inouye M. Identification of the promoter region of the Escherichia coli major cold shock gene, cspA. J Bacteriol. 1992 Jun;174(12):3867–3873. doi: 10.1128/jb.174.12.3867-3873.1992. [DOI] [PMC free article] [PubMed] [Google Scholar]
  20. VanBogelen R. A., Neidhardt F. C. Ribosomes as sensors of heat and cold shock in Escherichia coli. Proc Natl Acad Sci U S A. 1990 Aug;87(15):5589–5593. doi: 10.1073/pnas.87.15.5589. [DOI] [PMC free article] [PubMed] [Google Scholar]
  21. Vlasuk G. P., Inouye S., Ito H., Itakura K., Inouye M. Effects of the complete removal of basic amino acid residues from the signal peptide on secretion of lipoprotein in Escherichia coli. J Biol Chem. 1983 Jun 10;258(11):7141–7148. [PubMed] [Google Scholar]
  22. Willimsky G., Bang H., Fischer G., Marahiel M. A. Characterization of cspB, a Bacillus subtilis inducible cold shock gene affecting cell viability at low temperatures. J Bacteriol. 1992 Oct;174(20):6326–6335. doi: 10.1128/jb.174.20.6326-6335.1992. [DOI] [PMC free article] [PubMed] [Google Scholar]
  23. Wistow G. Cold shock and DNA binding. Nature. 1990 Apr 26;344(6269):823–824. doi: 10.1038/344823c0. [DOI] [PubMed] [Google Scholar]
  24. Wolffe A. P., Tafuri S., Ranjan M., Familari M. The Y-box factors: a family of nucleic acid binding proteins conserved from Escherichia coli to man. New Biol. 1992 Apr;4(4):290–298. [PubMed] [Google Scholar]

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