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. 1993 Nov;175(21):7074–7080. doi: 10.1128/jb.175.21.7074-7080.1993

A cytoplasmic protein, NfrC, is required for bacteriophage N4 adsorption.

D R Kiino 1, R Licudine 1, K Wilt 1, D H Yang 1, L B Rothman-Denes 1
PMCID: PMC206835  PMID: 8226648

Abstract

At least four genes are required for irreversible adsorption of bacteriophage N4. nfrA and nfrB have been characterized previously and encode an outer membrane protein and inner membrane protein, respectively. The nfrC gene product is characterized in detail in this study. We have mapped the nfrD locus to min 52 on the Escherichia coli linkage map. Maxicell analysis of nfrC and a null allele (nfrC2) cloned into a high-copy-number plasmid shows its gene product to be 42 kDa in size. We determined the nfrC nucleotide sequence which predicts a gene product of 42 kDa. Western blots (immunoblots) of Escherichia coli proteins after cellular fractionation show NfrC to be a cytoplasmic protein which is required for irreversible bacteriophage N4 adsorption, an event occurring at the cell surface.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Alting-Mees M. A., Short J. M. pBluescript II: gene mapping vectors. Nucleic Acids Res. 1989 Nov 25;17(22):9494–9494. doi: 10.1093/nar/17.22.9494. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Bachmann B. J. Linkage map of Escherichia coli K-12, edition 7. Microbiol Rev. 1983 Jun;47(2):180–230. doi: 10.1128/mr.47.2.180-230.1983. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Boulanger P., Letellier L. Characterization of ion channels involved in the penetration of phage T4 DNA into Escherichia coli cells. J Biol Chem. 1988 Jul 15;263(20):9767–9775. [PubMed] [Google Scholar]
  4. Boulanger P., Letellier L. Ion channels are likely to be involved in the two steps of phage T5 DNA penetration into Escherichia coli cells. J Biol Chem. 1992 Feb 15;267(5):3168–3172. [PubMed] [Google Scholar]
  5. Daniels D. L., Plunkett G., 3rd, Burland V., Blattner F. R. Analysis of the Escherichia coli genome: DNA sequence of the region from 84.5 to 86.5 minutes. Science. 1992 Aug 7;257(5071):771–778. doi: 10.1126/science.1379743. [DOI] [PubMed] [Google Scholar]
  6. Devereux J., Haeberli P., Smithies O. A comprehensive set of sequence analysis programs for the VAX. Nucleic Acids Res. 1984 Jan 11;12(1 Pt 1):387–395. doi: 10.1093/nar/12.1part1.387. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Dower W. J., Miller J. F., Ragsdale C. W. High efficiency transformation of E. coli by high voltage electroporation. Nucleic Acids Res. 1988 Jul 11;16(13):6127–6145. doi: 10.1093/nar/16.13.6127. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Elliott J., Arber W. E. coli K-12 pel mutants, which block phage lambda DNA injection, coincide with ptsM, which determines a component of a sugar transport system. Mol Gen Genet. 1978 Apr 25;161(1):1–8. doi: 10.1007/BF00266608. [DOI] [PubMed] [Google Scholar]
  9. Erni B., Zanolari B., Kocher H. P. The mannose permease of Escherichia coli consists of three different proteins. Amino acid sequence and function in sugar transport, sugar phosphorylation, and penetration of phage lambda DNA. J Biol Chem. 1987 Apr 15;262(11):5238–5247. [PubMed] [Google Scholar]
  10. Falco S. C., Laan K. V., Rothman-Denes L. B. Virion-associated RNA polymerase required for bacteriophage N4 development. Proc Natl Acad Sci U S A. 1977 Feb;74(2):520–523. doi: 10.1073/pnas.74.2.520. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Gloor G., Chaconas G. The bacteriophage Mu N gene encodes the 64-kDa virion protein which is injected with, and circularizes, infecting Mu DNA. J Biol Chem. 1986 Dec 15;261(35):16682–16688. [PubMed] [Google Scholar]
  12. Guihard G., Boulanger P., Letellier L. Involvement of phage T5 tail proteins and contact sites between the outer and inner membrane of Escherichia coli in phage T5 DNA injection. J Biol Chem. 1992 Feb 15;267(5):3173–3178. [PubMed] [Google Scholar]
  13. Hancock R. E., Hantke K., Braun V. Iron transport of Escherichia coli K-12: involvement of the colicin B receptor and of a citrate-inducible protein. J Bacteriol. 1976 Sep;127(3):1370–1375. doi: 10.1128/jb.127.3.1370-1375.1976. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Henikoff S. Unidirectional digestion with exonuclease III creates targeted breakpoints for DNA sequencing. Gene. 1984 Jun;28(3):351–359. doi: 10.1016/0378-1119(84)90153-7. [DOI] [PubMed] [Google Scholar]
  15. Ito K., Sato T., Yura T. Synthesis and assembly of the membrane proteins in E. coli. Cell. 1977 Jul;11(3):551–559. doi: 10.1016/0092-8674(77)90073-3. [DOI] [PubMed] [Google Scholar]
  16. Kiino D. R., Rothman-Denes L. B. Genetic analysis of bacteriophage N4 adsorption. J Bacteriol. 1989 Sep;171(9):4595–4602. doi: 10.1128/jb.171.9.4595-4602.1989. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Kiino D. R., Singer M. S., Rothman-Denes L. B. Two overlapping genes encoding membrane proteins required for bacteriophage N4 adsorption. J Bacteriol. 1993 Nov;175(21):7081–7085. doi: 10.1128/jb.175.21.7081-7085.1993. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. Kim K., Yoo O. J. Two subunits of mannose permease, II-PMan and II-MMan, of Escherichia coli mediate coliphage N4 infection. Biochem Int. 1989 Mar;18(3):545–549. [PubMed] [Google Scholar]
  19. Kohara Y., Akiyama K., Isono K. The physical map of the whole E. coli chromosome: application of a new strategy for rapid analysis and sorting of a large genomic library. Cell. 1987 Jul 31;50(3):495–508. doi: 10.1016/0092-8674(87)90503-4. [DOI] [PubMed] [Google Scholar]
  20. Kuhn H. M., Meier-Dieter U., Mayer H. ECA, the enterobacterial common antigen. FEMS Microbiol Rev. 1988 Sep;4(3):195–222. doi: 10.1111/j.1574-6968.1988.tb02743.x. [DOI] [PubMed] [Google Scholar]
  21. Laemmli U. K. Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature. 1970 Aug 15;227(5259):680–685. doi: 10.1038/227680a0. [DOI] [PubMed] [Google Scholar]
  22. Manoil C., Beckwith J. TnphoA: a transposon probe for protein export signals. Proc Natl Acad Sci U S A. 1985 Dec;82(23):8129–8133. doi: 10.1073/pnas.82.23.8129. [DOI] [PMC free article] [PubMed] [Google Scholar]
  23. Mizusawa S., Ward D. F. A bacteriophage lambda vector for cloning with BamHI and Sau3A. Gene. 1982 Dec;20(3):317–322. doi: 10.1016/0378-1119(82)90200-1. [DOI] [PubMed] [Google Scholar]
  24. Nagai K., Thøgersen H. C. Synthesis and sequence-specific proteolysis of hybrid proteins produced in Escherichia coli. Methods Enzymol. 1987;153:461–481. doi: 10.1016/0076-6879(87)53072-5. [DOI] [PubMed] [Google Scholar]
  25. Novick S. L., Baldeschwieler J. D. Fluorescence measurement of the kinetics of DNA injection by bacteriophage lambda into liposomes. Biochemistry. 1988 Oct 4;27(20):7919–7924. doi: 10.1021/bi00420a050. [DOI] [PubMed] [Google Scholar]
  26. O'Farrell P. H. High resolution two-dimensional electrophoresis of proteins. J Biol Chem. 1975 May 25;250(10):4007–4021. [PMC free article] [PubMed] [Google Scholar]
  27. Puspurs A. H., Trun N. J., Reeve J. N. Bacteriophage Mu DNA circularizes following infection of Escherichia coli. EMBO J. 1983;2(3):345–352. doi: 10.1002/j.1460-2075.1983.tb01429.x. [DOI] [PMC free article] [PubMed] [Google Scholar]
  28. Sancar A., Hack A. M., Rupp W. D. Simple method for identification of plasmid-coded proteins. J Bacteriol. 1979 Jan;137(1):692–693. doi: 10.1128/jb.137.1.692-693.1979. [DOI] [PMC free article] [PubMed] [Google Scholar]
  29. Sanger F., Nicklen S., Coulson A. R. DNA sequencing with chain-terminating inhibitors. Proc Natl Acad Sci U S A. 1977 Dec;74(12):5463–5467. doi: 10.1073/pnas.74.12.5463. [DOI] [PMC free article] [PubMed] [Google Scholar]
  30. Studier F. W., Moffatt B. A. Use of bacteriophage T7 RNA polymerase to direct selective high-level expression of cloned genes. J Mol Biol. 1986 May 5;189(1):113–130. doi: 10.1016/0022-2836(86)90385-2. [DOI] [PubMed] [Google Scholar]
  31. Studier F. W., Rosenberg A. H., Dunn J. J., Dubendorff J. W. Use of T7 RNA polymerase to direct expression of cloned genes. Methods Enzymol. 1990;185:60–89. doi: 10.1016/0076-6879(90)85008-c. [DOI] [PubMed] [Google Scholar]
  32. Webster R. E. The tol gene products and the import of macromolecules into Escherichia coli. Mol Microbiol. 1991 May;5(5):1005–1011. doi: 10.1111/j.1365-2958.1991.tb01873.x. [DOI] [PubMed] [Google Scholar]
  33. Yehle C. O. Genome-linked protein associated with the 5' termini of bacteriophage phi29 DNA. J Virol. 1978 Sep;27(3):776–783. doi: 10.1128/jvi.27.3.776-783.1978. [DOI] [PMC free article] [PubMed] [Google Scholar]
  34. Zagursky R. J., Berman M. L. Cloning vectors that yield high levels of single-stranded DNA for rapid DNA sequencing. Gene. 1984 Feb;27(2):183–191. doi: 10.1016/0378-1119(84)90139-2. [DOI] [PubMed] [Google Scholar]

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