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. 1993 Nov;175(21):7115–7118. doi: 10.1128/jb.175.21.7115-7118.1993

Use of Salmonella phage P22 for transduction in Escherichia coli.

B L Neal 1, P K Brown 1, P R Reeves 1
PMCID: PMC206843  PMID: 8226656

Abstract

A cosmid (pPR1347) carrying both the rfb gene cluster and the rfc gene of a Salmonella group B serovar has been constructed; Escherichia coli K-12 strains carrying this cosmid produce long-chain O antigen, are sensitive to phage P22, and can be transduced by P22. Some of the benefits of P22 transduction are now available for studying E. coli and potentially other genera.

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Selected References

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