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. 1993 Dec;175(23):7711–7714. doi: 10.1128/jb.175.23.7711-7714.1993

The pss and psd genes are required for motility and chemotaxis in Escherichia coli.

W Shi 1, M Bogdanov 1, W Dowhan 1, D R Zusman 1
PMCID: PMC206932  PMID: 8244943

Abstract

Mutants of Escherichia coli defective in phosphatidylserine synthase (encoded by pss) and phosphatidylserine decarboxylase (encoded by psd) make cell membranes deficient in phosphatidylethanolamine. In this report we show that wild-type pss and psd genes are required for motility and chemotaxis. Null mutants or strains with temperature-sensitive pss or psd mutations grown at high temperature (35 degrees C) were nonmotile. They lacked flagella and showed reduced rates of transcription of the flhD master operon (encoding FlhD and FlhC), the fliA operon (encoding sigma F), and the fliC operon (encoding flagellin). At low temperature (25 degrees C), the temperature-sensitive mutant cells showed motility and chemotaxis but at reduced levels. The extent of the motility and chemotaxis defects in the mutants was correlated with the amount of phosphatidylethanolamine in the membranes, suggesting a link between membrane phospholipid composition and expression of the flagellum chemotaxis regulon.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Adler J. A method for measuring chemotaxis and use of the method to determine optimum conditions for chemotaxis by Escherichia coli. J Gen Microbiol. 1973 Jan;74(1):77–91. doi: 10.1099/00221287-74-1-77. [DOI] [PubMed] [Google Scholar]
  2. Adler J. Chemotaxis in bacteria. Science. 1966 Aug 12;153(3737):708–716. doi: 10.1126/science.153.3737.708. [DOI] [PubMed] [Google Scholar]
  3. Adler J., Templeton B. The effect of environmental conditions on the motility of Escherichia coli. J Gen Microbiol. 1967 Feb;46(2):175–184. doi: 10.1099/00221287-46-2-175. [DOI] [PubMed] [Google Scholar]
  4. Bar Tana J., Howlett B. J., Koshland D. E., Jr Flagellar formation in Escherichia coli electron transport mutants. J Bacteriol. 1977 May;130(2):787–792. doi: 10.1128/jb.130.2.787-792.1977. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Casadaban M. J. Transposition and fusion of the lac genes to selected promoters in Escherichia coli using bacteriophage lambda and Mu. J Mol Biol. 1976 Jul 5;104(3):541–555. doi: 10.1016/0022-2836(76)90119-4. [DOI] [PubMed] [Google Scholar]
  6. DeChavigny A., Heacock P. N., Dowhan W. Sequence and inactivation of the pss gene of Escherichia coli. Phosphatidylethanolamine may not be essential for cell viability. J Biol Chem. 1991 Mar 15;266(8):5323–5332. [PubMed] [Google Scholar]
  7. Dobrogosz W. J., Hamilton P. B. The role of cyclic AMP in chemotaxis in Escherichia coli. Biochem Biophys Res Commun. 1971 Jan 22;42(2):202–207. doi: 10.1016/0006-291x(71)90088-x. [DOI] [PubMed] [Google Scholar]
  8. Farr S. B., Arnosti D. N., Chamberlin M. J., Ames B. N. An apaH mutation causes AppppA to accumulate and affects motility and catabolite repression in Escherichia coli. Proc Natl Acad Sci U S A. 1989 Jul;86(13):5010–5014. doi: 10.1073/pnas.86.13.5010. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Fiedler W., Rotering H. Properties of Escherichia coli mutants lacking membrane-derived oligosaccharides. J Biol Chem. 1988 Oct 15;263(29):14684–14689. [PubMed] [Google Scholar]
  10. Hawrot E., Kennedy E. P. Phospholipid composition and membrane function in phosphatidylserine decarboxylase mutants of Escherichia coli. J Biol Chem. 1978 Nov 25;253(22):8213–8220. [PubMed] [Google Scholar]
  11. Iino T. Genetics of structure and function of bacterial flagella. Annu Rev Genet. 1977;11:161–182. doi: 10.1146/annurev.ge.11.120177.001113. [DOI] [PubMed] [Google Scholar]
  12. Jones C. J., Aizawa S. The bacterial flagellum and flagellar motor: structure, assembly and function. Adv Microb Physiol. 1991;32:109–172. doi: 10.1016/s0065-2911(08)60007-7. [DOI] [PubMed] [Google Scholar]
  13. Komeda Y. Fusions of flagellar operons to lactose genes on a mu lac bacteriophage. J Bacteriol. 1982 Apr;150(1):16–26. doi: 10.1128/jb.150.1.16-26.1982. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Komeda Y., Suzuki H., Ishidsu J. I., Iino T. The role of cAMP in flagellation of Salmonella typhimurium. Mol Gen Genet. 1976 Dec 31;142(4):289–298. doi: 10.1007/BF00271253. [DOI] [PubMed] [Google Scholar]
  15. Komeda Y. Transcriptional control of flagellar genes in Escherichia coli K-12. J Bacteriol. 1986 Dec;168(3):1315–1318. doi: 10.1128/jb.168.3.1315-1318.1986. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Li C., Louise C. J., Shi W., Adler J. Adverse conditions which cause lack of flagella in Escherichia coli. J Bacteriol. 1993 Apr;175(8):2229–2235. doi: 10.1128/jb.175.8.2229-2235.1993. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Li Q. X., Dowhan W. Studies on the mechanism of formation of the pyruvate prosthetic group of phosphatidylserine decarboxylase from Escherichia coli. J Biol Chem. 1990 Mar 5;265(7):4111–4115. [PubMed] [Google Scholar]
  18. Ohnishi K., Kutsukake K., Suzuki H., Iino T. Gene fliA encodes an alternative sigma factor specific for flagellar operons in Salmonella typhimurium. Mol Gen Genet. 1990 Apr;221(2):139–147. doi: 10.1007/BF00261713. [DOI] [PubMed] [Google Scholar]
  19. Ohta A., Waggoner K., Louie K., Dowhan W. Cloning of genes involved in membrane lipid synthesis. Effects of amplification of phosphatidylserine synthase in Escherichia coli. J Biol Chem. 1981 Mar 10;256(5):2219–2225. [PubMed] [Google Scholar]
  20. Raetz C. R., Dowhan W. Biosynthesis and function of phospholipids in Escherichia coli. J Biol Chem. 1990 Jan 25;265(3):1235–1238. [PubMed] [Google Scholar]
  21. Raetz C. R., Foulds J. Envelope composition and antibiotic hypersensitivity of Escherichia coli mutants defective in phosphatidylserine synthetase. J Biol Chem. 1977 Aug 25;252(16):5911–5915. [PubMed] [Google Scholar]
  22. Raetz C. R., Kantor G. D., Nishijima M., Newman K. F. Cardiolipin accumulation in the inner and outer membranes of Escherichia coli mutants defective in phosphatidylserine synthetase. J Bacteriol. 1979 Aug;139(2):544–551. doi: 10.1128/jb.139.2.544-551.1979. [DOI] [PMC free article] [PubMed] [Google Scholar]
  23. Raetz C. R. Phosphatidylserine synthetase mutants of Escherichia coli. Genetic mapping and membrane phospholipid composition. J Biol Chem. 1976 Jun 10;251(11):3242–3249. [PubMed] [Google Scholar]
  24. Sager B. M., Sekelsky J. J., Matsumura P., Adler J. Use of a computer to assay motility in bacteria. Anal Biochem. 1988 Sep;173(2):271–277. doi: 10.1016/0003-2697(88)90189-3. [DOI] [PubMed] [Google Scholar]
  25. Shi W., Li C., Louise C. J., Adler J. Mechanism of adverse conditions causing lack of flagella in Escherichia coli. J Bacteriol. 1993 Apr;175(8):2236–2240. doi: 10.1128/jb.175.8.2236-2240.1993. [DOI] [PMC free article] [PubMed] [Google Scholar]
  26. Shi W., Zhou Y., Wild J., Adler J., Gross C. A. DnaK, DnaJ, and GrpE are required for flagellum synthesis in Escherichia coli. J Bacteriol. 1992 Oct;174(19):6256–6263. doi: 10.1128/jb.174.19.6256-6263.1992. [DOI] [PMC free article] [PubMed] [Google Scholar]
  27. Shi W., Zusman D. R. The two motility systems of Myxococcus xanthus show different selective advantages on various surfaces. Proc Natl Acad Sci U S A. 1993 Apr 15;90(8):3378–3382. doi: 10.1073/pnas.90.8.3378. [DOI] [PMC free article] [PubMed] [Google Scholar]
  28. Shibuya I., Miyazaki C., Ohta A. Alteration of phospholipid composition by combined defects in phosphatidylserine and cardiolipin synthases and physiological consequences in Escherichia coli. J Bacteriol. 1985 Mar;161(3):1086–1092. doi: 10.1128/jb.161.3.1086-1092.1985. [DOI] [PMC free article] [PubMed] [Google Scholar]
  29. Silverman M., Simon M. Characterization of Escherichia coli flagellar mutants that are insensitive to catabolite repression. J Bacteriol. 1974 Dec;120(3):1196–1203. doi: 10.1128/jb.120.3.1196-1203.1974. [DOI] [PMC free article] [PubMed] [Google Scholar]
  30. Yokota T., Gots J. S. Requirement of adenosine 3', 5'-cyclic phosphate for flagella formation in Escherichia coli and Salmonella typhimurium. J Bacteriol. 1970 Aug;103(2):513–516. doi: 10.1128/jb.103.2.513-516.1970. [DOI] [PMC free article] [PubMed] [Google Scholar]

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