Skip to main content
PMC home page
British Journal of Pharmacology logoLink to British Journal of Pharmacology
. 1981 Jun;73(2):379–384. doi: 10.1111/j.1476-5381.1981.tb10432.x

Identification of a purine (P2) receptor linked to ion transport in a cultured renal (MDCK) epithelium.

N L Simmons
PMCID: PMC2071660  PMID: 6263386

Abstract

1 Exogenous adenosine triphosphate (ATP) stimulates the short circuit current (SCC) in a cultured renal-derived epithelium (MDCK). Half-maximal stimulation is achieved at 1.91 X 10(-5) M ATP. 2 It is suggested that ATP interacts with a P2 purine receptor upon the basis of (a) agonist potency (ATP greater than adenosine diphosphate much greater than adenosine monophosphate, adenosine; ATP greater than uridine triphosphate greater than inosine triphosphate much greater than cytosine triphosphate, guanosine triphosphate); (b) the inhibition of the ATP response by quinidine (1 X 10(-3) M) but not by theophylline (1 X 10(-3) M). 3 Indomethacin (1 X 10(-5) M) inhibits the response of the cultured epithelium to ATP. 4 Prostaglandin E1 (PGE1) stimulates SCC but potentiates the effect of ATP on SCC. The divalent cationic ionophore A23187 (1 X 10(-6) M) transiently stimulates SCC itself and abolishes ATP-induced stimulation of the SCC.

Full text

PDF
381

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Aiton J. F., Lamb J. F. The effect of exogenous adenosine triphosphate on potassium movements in HeLa cells. Q J Exp Physiol Cogn Med Sci. 1980 Jan;65(1):47–62. doi: 10.1113/expphysiol.1980.sp002491. [DOI] [PubMed] [Google Scholar]
  2. Arch J. R., Newsholme E. A. The control of the metabolism and the hormonal role of adenosine. Essays Biochem. 1978;14:82–123. [PubMed] [Google Scholar]
  3. Barker G., Simmons N. L. Dog kidney cell monolayers can display properties similar to high-resistance epithelia [proceedings]. J Physiol. 1979 Apr;289:33P–34P. [PubMed] [Google Scholar]
  4. Boyd I. A., Forrester T. The release of adenosine triphosphate from frog skeletal muscle in vitro. J Physiol. 1968 Nov;199(1):115–135. doi: 10.1113/jphysiol.1968.sp008642. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Cereijido M., Robbins E. S., Dolan W. J., Rotunno C. A., Sabatini D. D. Polarized monolayers formed by epithelial cells on a permeable and translucent support. J Cell Biol. 1978 Jun;77(3):853–880. doi: 10.1083/jcb.77.3.853. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Ilundain A., Naftalin R. J. Role of Ca(2+)-dependent regulator protein in intestinal secretion. Nature. 1979 May 31;279(5712):446–448. doi: 10.1038/279446a0. [DOI] [PubMed] [Google Scholar]
  7. Kohn P. G., Newey H., Smyth D. H. The effect of adenosine triphosphate on the transmural potential in rat small intestine. J Physiol. 1970 May;208(1):203–220. doi: 10.1113/jphysiol.1970.sp009114. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Levine L. Chemical carcinogens stimulate canine kidney (MDCK) cells to produce prostaglandins. Nature. 1977 Aug 4;268(5619):447–448. doi: 10.1038/268447a0. [DOI] [PubMed] [Google Scholar]
  9. Liang C. T., Sacktor B. Preparation of renal cortex basal-lateral and bursh border membranes. Localization of adenylate cyclase and guanylate cyclase activities. Biochim Biophys Acta. 1977 May 2;466(3):474–487. doi: 10.1016/0005-2736(77)90340-6. [DOI] [PubMed] [Google Scholar]
  10. Misfeldt D. S., Hamamoto S. T., Pitelka D. R. Transepithelial transport in cell culture. Proc Natl Acad Sci U S A. 1976 Apr;73(4):1212–1216. doi: 10.1073/pnas.73.4.1212. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Rorive G., Kleinzeller A. The effect of ATP and Ca 2+ on the cell volume in isolated kidney tubules. Biochim Biophys Acta. 1972 Jul 3;274(1):226–239. doi: 10.1016/0005-2736(72)90296-9. [DOI] [PubMed] [Google Scholar]
  12. Simmons N. L. Ion transport in high-resistance dog-kidney cell monolayers: the effect of adenosine 5', triphosphate [proceedings]. J Physiol. 1979 May;290(2):28P–29P. [PubMed] [Google Scholar]
  13. Simmons N. L., Naftalin R. J. Bidirectional sodium ion movements via the paracellular and transcellular routes across short-circuited rabbit ileum. Biochim Biophys Acta. 1976 Oct 19;448(3):426–450. doi: 10.1016/0005-2736(76)90298-4. [DOI] [PubMed] [Google Scholar]
  14. Vane J. R. Inhibition of prostaglandin synthesis as a mechanism of action for aspirin-like drugs. Nat New Biol. 1971 Jun 23;231(25):232–235. doi: 10.1038/newbio231232a0. [DOI] [PubMed] [Google Scholar]
  15. Westcott K. R., Engelhard V. H., Storm D. R. Production of cyclic AMP from extracellular ATP by intact LM cells. Biochim Biophys Acta. 1979 Feb 19;583(1):47–54. doi: 10.1016/0304-4165(79)90308-8. [DOI] [PubMed] [Google Scholar]
  16. Yount R. G., Babcock D., Ballantyne W., Ojala D. Adenylyl imidodiphosphate, an adenosine triphosphate analog containing a P--N--P linkage. Biochemistry. 1971 Jun 22;10(13):2484–2489. doi: 10.1021/bi00789a009. [DOI] [PubMed] [Google Scholar]

Articles from British Journal of Pharmacology are provided here courtesy of The British Pharmacological Society

RESOURCES