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. 1981 Oct;74(2):389–397. doi: 10.1111/j.1476-5381.1981.tb09983.x

Inhibition by apomorphine of the potassium-evoked release of [3H]-γ-aminobutyric acid from the rat substantia nigra in vitro

Sonia Arbilla, Linda A Kamal, SZ Langer
PMCID: PMC2071740  PMID: 7317688

Abstract

1 The spontaneous and potassium-evoked release of tritium from the rat substantia nigra prelabelled with [3H]-γ-aminobutyric acid [3H]-GABA were assessed in vitro under conditions of superfusion.

2 Kainic acid lesions performed in the right caudate nucleus resulted in a 70% reduction in the ability of the homolateral nigral cells to take up and retain [3H]-GABA when compared with the unlesioned side. The potassium-evoked release of [3H]-GABA remained proportional to the radioactivity retained in the tissue suggesting that the nigral GABA neurones that survived kainic acid treatment were still functional.

3 The spontaneous outflow of [3H]-GABA was significantly increased by exposure to different concentrations of exogenous GABA (10 to 1000 μM) when amino-oxyacetic acid was present in the incubation medium.

4 Apomorphine in concentrations ranging from 1 to 30 μM inhibited the calcium-dependent release of [3H]-GABA induced by 1 min exposure to 30 mM K+. These concentrations of apomorphine did not affect the spontaneous outflow of radioactivity. In vivo administration of haloperidol 0.2 mg/kg antagonized the in vitro inhibition by apomorphine of the K+-evoked release of [3H]-GABA.

5 The results obtained with apomorphine and haloperidol suggest the presence of presynaptic dopamine-like inhibitory receptors in gabaergic nerve terminals.

6 Dopamine in concentrations ranging up to 300 μM did not modify either the spontaneous or the K+-evoked release of [3H]-GABA from the substantia nigra. These concentrations of dopamine effectively displaced [3H]-dopamine recently taken up into the substantia nigra.

7 Our results do not support the view that dendritic release of dopamine from the substantia nigra might be involved in the physiological modulation of the spontaneous or the stimulation-evoked release of GABA.

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Selected References

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