Abstract
Organ cultures of pig or calf trachea were infected with influenza viruses, parainfluenza viruses and a rhinovirus. Infection of calf tracheal cultures with a bovine rhinovirus and with parainfluenza virus type 3 produced considerable epithelial damage. Influenza strains and Sendai virus in calf tracheal cultures and swine influenza virus in pig tracheal cultures damaged the epithelium much less strikingly. In all the infections studied virus production continued undiminished for at least two weeks. Persistent infection of porcine cultures with swine influenza virus was followed for 24 days. Interferon was demonstrable in the infected cultures for 6 days, but on challenge with Sendai virus at various times up to the 19th day of infection these cultures yielded markedly less of the superinfecting virus than did control cultures. Treatment of swine influenza-infected cultures with porcine interferon or with swine influenza antiserum for 7 days did not eliminate the infection. The bearing that these infections in organ culture may have on the mechanisms of respiratory infections in vivo is discussed.
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