Skip to main content
PMC home page
British Journal of Experimental Pathology logoLink to British Journal of Experimental Pathology
. 1969 Apr;50(2):200–208.

Mitochondrial Antibodies in Primary Biliary Cirrhosis

II. The Complement Fixing Antigen as a Component of Mitochondrial Inner Membranes

P A Berg, U Muscatello, R W Horne, I M Roitt, Deborah Doniach
PMCID: PMC2072148  PMID: 5772074

Abstract

The autoantibodies found in the sera of patients with primary biliary cirrhosis have been shown to react with a component of the mitochondrial inner membranes. Outer membranes were inactive. The purity of the inner and outer membrane fractions obtained by 2 different methods was assessed by electron microscopy and marker enzyme tests. Using negative-staining it was possible to visualize antibody binding to mitochondrial membranes. At high resolution it could be seen that the 90° particles on the cristal membranes were not involved in the reaction with antibody, but it was not possible to establish in the present studies the precise antigenic site upon the mitochondrial inner membranes.

Full text

PDF
201

Images in this article

204-11Figs. 8a-8b
on p.204-11
204-3Figs. 2a-2b
on p.204-3
204-9Figs. 7a-7b
on p.204-9
204-13Figs. 9a-9c
on p.204-13
204-1Fig. 1a-1b
on p.204-1
204-7Figs. 5-6
on p.204-7
204-5Figs. 3-4
on p.204-5

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. BARTLETT G. R. Phosphorus assay in column chromatography. J Biol Chem. 1959 Mar;234(3):466–468. [PubMed] [Google Scholar]
  2. Berg P. A., Doniach D., Roitt I. M. Mitochondrial antibodies in primary biliary cirrhosis. I. Localization of the antigen to mitochondrial membranes. J Exp Med. 1967 Aug 1;126(2):277–290. doi: 10.1084/jem.126.2.277. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Cunningham W. P., Crane F. L., Sottocasa G. L. The relation between structure and function in electron-transport systems. I. Sequential release of nicotinamide-adenine dinucleotide dehydrogenase activity by a series of physical treatments. Biochim Biophys Acta. 1965 Nov 22;110(2):265–276. doi: 10.1016/s0926-6593(65)80034-0. [DOI] [PubMed] [Google Scholar]
  4. Doniach D., Roitt I. M., Walker J. G., Sherlock S. Tissue antibodies in primary biliary cirrhosis, active chronic (lupoid) hepatitis, cryptogenic cirrhosis and other liver diseases and their clinical implications. Clin Exp Immunol. 1966 Jul;1(3):237–262. [PMC free article] [PubMed] [Google Scholar]
  5. Green D. E., Allmann D. W., Harris R. A., Tan W. C. Enzyme localization in the inner and outer mitochondrial membranes. Biochem Biophys Res Commun. 1968 May 10;31(3):368–378. doi: 10.1016/0006-291x(68)90485-3. [DOI] [PubMed] [Google Scholar]
  6. HARRIS W. J. Holey films for electron microscopy. Nature. 1962 Nov 3;196:499–500. doi: 10.1038/196499b0. [DOI] [PubMed] [Google Scholar]
  7. Lindberg O., de Pierre J., Rylander E., Afzelius B. A. Studies of the mitochondrial energy-transfer system of brown adipose tissue. J Cell Biol. 1967 Jul;34(1):293–310. doi: 10.1083/jcb.34.1.293. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Parsons D. F., Williams G. R., Chance B. Characteristics of isolated and purified preparations of the outer and inner membranes of mitochondria. Ann N Y Acad Sci. 1966 Jul 14;137(2):643–666. doi: 10.1111/j.1749-6632.1966.tb50188.x. [DOI] [PubMed] [Google Scholar]
  9. SLATER T. F., PLANTEROSE D. N. An assay procedure for a succinate-neotetrazolium-reductase system. Biochem J. 1960 Mar;74:591–596. doi: 10.1042/bj0740591. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Schnaitman C., Erwin V. G., Greenawalt J. W. The submitochondrial localization of monoamine oxidase. An enzymatic marker for the outer membrane of rat liver mitochondria. J Cell Biol. 1967 Mar;32(3):719–735. doi: 10.1083/jcb.32.3.719. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Sottocasa G. L., Kuylenstierna B., Ernster L., Bergstrand A. An electron-transport system associated with the outer membrane of liver mitochondria. A biochemical and morphological study. J Cell Biol. 1967 Feb;32(2):415–438. doi: 10.1083/jcb.32.2.415. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Tipton K. F. The sub-mitochondrial localization of monoamine oxidase in rat liver and brain. Biochim Biophys Acta. 1967;135(5):910–920. doi: 10.1016/0005-2736(67)90060-0. [DOI] [PubMed] [Google Scholar]
  13. Tubbs P. K., Garland P. B. Membranes and fatty acid metabolism. Br Med Bull. 1968 May;24(2):158–164. doi: 10.1093/oxfordjournals.bmb.a070619. [DOI] [PubMed] [Google Scholar]
  14. WALKER J. G., DONIACH D., ROITT I. M., SHERLOCK S. SEROLOGICAL TESTS IN DIAGNOSIS OF PRIMARY BILIARY CIRRHOSIS. Lancet. 1965 Apr 17;1(7390):827–831. doi: 10.1016/s0140-6736(65)91372-3. [DOI] [PubMed] [Google Scholar]

Articles from British Journal of Experimental Pathology are provided here courtesy of Wiley

RESOURCES