Skip to main content
PMC home page
British Journal of Experimental Pathology logoLink to British Journal of Experimental Pathology
. 1969 Dec;50(6):540–547.

Increased Incorporation of 3H-thymidine into Rat Liver DNA After Handling of Liver Lobes

K Weinbren, Gillian M Arden, G A Stirling
PMCID: PMC2072165  PMID: 5364385

Abstract

Handling of part of the rat liver without resection or interference with the vascular supply was associated with an increased rate of incorporation of 3H-thymidine into DNA. The response curve was distinctive and different in shape and in magnitude from that which occurs after partial hepatectomy. Incorporation was greater in the peripheral rather than central parts of the liver, was more striking after i.p. rather than intravenous administration of isotope and was associated with a proliferation of hepatic capsular cells. Radioautographs showed activity in capsular cells, inflammatory cells and also in a few peripherally placed hepatocytes.

The possibility is canvassed that this phenomenon may have led to misinterpretation of results of experiments designed to test for the presence of humoral stimuli or inhibitors in restoration of the liver.

Full text

PDF
546

Images in this article

544-1Figs. 3-6
on p.544-1

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. ALLISON A. C., MALLUCCI L. LYSOSOMES IN DIVIDING CELLS, WITH SPECIAL REFERENCE TO LYMPHOCYTES. Lancet. 1964 Dec 26;2(7374):1371–1373. doi: 10.1016/s0140-6736(64)91162-6. [DOI] [PubMed] [Google Scholar]
  2. ALSTON W. C., THOMSON R. Y. HUMORAL AND LOCAL FACTORS IN LIVER REGENERATION. Cancer Res. 1963 Jul;23:901–905. [PubMed] [Google Scholar]
  3. BENACERRAF B., BILBEY D., BIOZZI G., HALPERN B. N., STIFFEL C. The measurement of liver blood flow in partially hepatectomized rats. J Physiol. 1957 Apr 30;136(2):287–293. doi: 10.1113/jphysiol.1957.sp005759. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. BUCHER N. L., SWAFFIELD M. N., DITROIA J. F. THE INFLUENCE OF AGE UPON THE INCORPORATION OF THYMIDINE-2-C14 INTO THE DNA OF REGENERATING RAT LIVER. Cancer Res. 1964 Apr;24:509–512. [PubMed] [Google Scholar]
  5. Banerjee A. K., Aikat B. K. Effect of liver regeneration on the portal pressure and vascular pattern in normal and cirrhotic rats. J Pathol Bacteriol. 1968 Oct;96(2):327–335. doi: 10.1002/path.1700960210. [DOI] [PubMed] [Google Scholar]
  6. Bucher N. L. Experimental aspects of hepatic regeneration. N Engl J Med. 1967 Sep 28;277(13):686–contd. doi: 10.1056/NEJM196709282771306. [DOI] [PubMed] [Google Scholar]
  7. GRILLO R. S., URSO P., O'BRIAN D. M. THE EFFECT OF DOSE ON THE INCORPORATION OF 3H-THYMIDINE IN THE NUCLEI OF THE LIVER CAPSULE CELLS IN THE NEWT, TRITURUS VIRIDESCENS. Exp Cell Res. 1965 Mar;37:683–685. doi: 10.1016/0014-4827(65)90216-8. [DOI] [PubMed] [Google Scholar]
  8. Grisham J. W., Leong G. F., Albright M. L., Emerson J. D. Effect of exchange transfusion on labeling of nuclei with thymidine-3-H and on mitosis in hepatocytes of normal and regenerating rat liver. Cancer Res. 1966 Jul;26(7):1476–1485. [PubMed] [Google Scholar]
  9. LEONG G. F., GRISHAM J. W., HOLE B. V., ALBRIGHT M. L. EFFECT OF PARTIAL HEPATECTOMY ON DNA SYNTHESIS AND MITOSIS IN HETEROTOPIC PARTIAL AUTOGRAFTS OF RAT LIVER. Cancer Res. 1964 Sep;24:1496–1501. [PubMed] [Google Scholar]
  10. LIEBERMAN I., SHORT J. HEPATIC BLOOD SUPPLY AND CONTROL OF DEOXYRIBONUCLEIC ACID SYNTHESIS IN LIVER. Am J Physiol. 1965 May;208:896–902. doi: 10.1152/ajplegacy.1965.208.5.896. [DOI] [PubMed] [Google Scholar]
  11. WEINBREN K., WOODWARD E. DELAYED INCORPORATION OF 32P FROM ORTHOPHOSPHATE INTO DEOXYRIBONUCLEIC ACID OF RAT LIVER AFTER SUBTOTAL HEPATECTOMY. Br J Exp Pathol. 1964 Aug;45:442–449. [PMC free article] [PubMed] [Google Scholar]
  12. van Gool J., Ladiges N. C. Production of foetal globulin after injury in rat and man. J Pathol. 1969 Jan;97(1):115–126. doi: 10.1002/path.1710970115. [DOI] [PubMed] [Google Scholar]

Articles from British Journal of Experimental Pathology are provided here courtesy of Wiley

RESOURCES