Skip to main content
PMC home page
Journal of Bacteriology logoLink to Journal of Bacteriology
. 1991 Feb;173(3):1120–1124. doi: 10.1128/jb.173.3.1120-1124.1991

Thermosensing ability of Trg and Tap chemoreceptors in Escherichia coli.

T Nara 1, L Lee 1, Y Imae 1
PMCID: PMC207232  PMID: 1991711

Abstract

The thermosensing ability of the Trg and Tap chemoreceptors in Escherichia coli was investigated after amplifying these receptors in a host strain lacking all four known chemoreceptors (Tar, Tsr, Trg, and Tap). Cells with an increased amount of either Trg or Tap showed mostly smooth swimming and no response to thermal stimuli. However, when the smooth-swimming bias of the cells was reduced by adding Trg- or Tap-mediated repellents, the cells showed clear changes in the swimming pattern upon temperature changes; Trg-containing cells showed tumbling at 23 degrees C but mostly smooth swimming at 32 degrees C, while Tap-containing cells showed smooth swimming at 20 degrees C but tumbling at 32 degrees C. These results indicate that although both Trg and Tap have the ability to sense thermal stimuli, Trg functions as a warm receptor, as reported previously for Tar and Tsr, while Tap functions as a cold receptor.

Full text

PDF
1121

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Hazelbauer G. L., Engström P. Parallel pathways for transduction of chemotactic signals in Escherichia coli. Nature. 1980 Jan 3;283(5742):98–100. doi: 10.1038/283098a0. [DOI] [PubMed] [Google Scholar]
  2. Hazelbauer G. L. The bacterial chemosensory system. Can J Microbiol. 1988 Apr;34(4):466–474. doi: 10.1139/m88-080. [DOI] [PubMed] [Google Scholar]
  3. Imae Y., Mizuno T., Maeda K. Chemosensory and thermosensory excitation in adaptation-deficient mutants of Escherichia coli. J Bacteriol. 1984 Jul;159(1):368–374. doi: 10.1128/jb.159.1.368-374.1984. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Imae Y., Oosawa K., Mizuno T., Kihara M., Macnab R. M. Phenol: a complex chemoeffector in bacterial chemotaxis. J Bacteriol. 1987 Jan;169(1):371–379. doi: 10.1128/jb.169.1.371-379.1987. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Kondoh H., Ball C. B., Adler J. Identification of a methyl-accepting chemotaxis protein for the ribose and galactose chemoreceptors of Escherichia coli. Proc Natl Acad Sci U S A. 1979 Jan;76(1):260–264. doi: 10.1073/pnas.76.1.260. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Krikos A., Conley M. P., Boyd A., Berg H. C., Simon M. I. Chimeric chemosensory transducers of Escherichia coli. Proc Natl Acad Sci U S A. 1985 Mar;82(5):1326–1330. doi: 10.1073/pnas.82.5.1326. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Lee L., Imae Y. Role of threonine residue 154 in ligand recognition of the tar chemoreceptor in Escherichia coli. J Bacteriol. 1990 Jan;172(1):377–382. doi: 10.1128/jb.172.1.377-382.1990. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Lee L., Mizuno T., Imae Y. Thermosensing properties of Escherichia coli tsr mutants defective in serine chemoreception. J Bacteriol. 1988 Oct;170(10):4769–4774. doi: 10.1128/jb.170.10.4769-4774.1988. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Maeda K., Imae Y., Shioi J. I., Oosawa F. Effect of temperature on motility and chemotaxis of Escherichia coli. J Bacteriol. 1976 Sep;127(3):1039–1046. doi: 10.1128/jb.127.3.1039-1046.1976. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Maeda K., Imae Y. Thermosensory transduction in Escherichia coli: inhibition of the thermoresponse by L-serine. Proc Natl Acad Sci U S A. 1979 Jan;76(1):91–95. doi: 10.1073/pnas.76.1.91. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Mizuno T., Imae Y. Conditional inversion of the thermoresponse in Escherichia coli. J Bacteriol. 1984 Jul;159(1):360–367. doi: 10.1128/jb.159.1.360-367.1984. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Oosawa K., Imae Y. Glycerol and ethylene glycol: members of a new class of repellents of Escherichia coli chemotaxis. J Bacteriol. 1983 Apr;154(1):104–112. doi: 10.1128/jb.154.1.104-112.1983. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Park C., Hazelbauer G. L. Mutations specifically affecting ligand interaction of the Trg chemosensory transducer. J Bacteriol. 1986 Jul;167(1):101–109. doi: 10.1128/jb.167.1.101-109.1986. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Weis R. M., Koshland D. E., Jr Chemotaxis in Escherichia coli proceeds efficiently from different initial tumble frequencies. J Bacteriol. 1990 Feb;172(2):1099–1105. doi: 10.1128/jb.172.2.1099-1105.1990. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Wolfe A. J., Berg H. C. Migration of bacteria in semisolid agar. Proc Natl Acad Sci U S A. 1989 Sep;86(18):6973–6977. doi: 10.1073/pnas.86.18.6973. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Wolfe A. J., Conley M. P., Kramer T. J., Berg H. C. Reconstitution of signaling in bacterial chemotaxis. J Bacteriol. 1987 May;169(5):1878–1885. doi: 10.1128/jb.169.5.1878-1885.1987. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Yamamoto K., Macnab R. M., Imae Y. Repellent response functions of the Trg and Tap chemoreceptors of Escherichia coli. J Bacteriol. 1990 Jan;172(1):383–388. doi: 10.1128/jb.172.1.383-388.1990. [DOI] [PMC free article] [PubMed] [Google Scholar]

Articles from Journal of Bacteriology are provided here courtesy of American Society for Microbiology (ASM)

RESOURCES