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. 1991 Feb;173(3):1339–1343. doi: 10.1128/jb.173.3.1339-1343.1991

Specificity of mini-Mu bacteriophage insertions in a small plasmid.

B A Castilho 1, M J Casadaban 1
PMCID: PMC207261  PMID: 1846863

Abstract

Target site selection for bacteriophage Mu transposition was studied in pools of over 10(7) independent mini-Mu insertions in pUC9, selected by transduction of the plasmid. Insertions in both orientations were clustered in three regions and, within these, at preferred sites.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Adzuma K., Mizuuchi K. Target immunity of Mu transposition reflects a differential distribution of Mu B protein. Cell. 1988 Apr 22;53(2):257–266. doi: 10.1016/0092-8674(88)90387-x. [DOI] [PubMed] [Google Scholar]
  2. Berg D. E., Schmandt M. A., Lowe J. B. Specificity of transposon Tn5 insertion. Genetics. 1983 Dec;105(4):813–828. doi: 10.1093/genetics/105.4.813. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Castilho B. A., Olfson P., Casadaban M. J. Plasmid insertion mutagenesis and lac gene fusion with mini-mu bacteriophage transposons. J Bacteriol. 1984 May;158(2):488–495. doi: 10.1128/jb.158.2.488-495.1984. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Craigie R., Mizuuchi M., Mizuuchi K. Site-specific recognition of the bacteriophage Mu ends by the Mu A protein. Cell. 1984 Dec;39(2 Pt 1):387–394. doi: 10.1016/0092-8674(84)90017-5. [DOI] [PubMed] [Google Scholar]
  5. Daniell E., Roberts R., Abelson J. Mutations in the lactose operon caused by bacteriophage Mu. J Mol Biol. 1972 Aug 14;69(1):1–8. doi: 10.1016/0022-2836(72)90019-8. [DOI] [PubMed] [Google Scholar]
  6. Darzins A., Kent N. E., Buckwalter M. S., Casadaban M. J. Bacteriophage Mu sites required for transposition immunity. Proc Natl Acad Sci U S A. 1988 Sep;85(18):6826–6830. doi: 10.1073/pnas.85.18.6826. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Halling S. M., Kleckner N. A symmetrical six-base-pair target site sequence determines Tn10 insertion specificity. Cell. 1982 Jan;28(1):155–163. doi: 10.1016/0092-8674(82)90385-3. [DOI] [PubMed] [Google Scholar]
  8. Kamp D., Kahmann R. Two pathways in bacteriophage Mu transposition? Cold Spring Harb Symp Quant Biol. 1981;45(Pt 1):329–336. doi: 10.1101/sqb.1981.045.01.046. [DOI] [PubMed] [Google Scholar]
  9. Klaer R., Kühn S., Fritz H. J., Tillmann E., Saint-Girons I., Habermann P., Pfeifer D., Starlinger P. Studies on transposition mechanisms and specificity of IS4. Cold Spring Harb Symp Quant Biol. 1981;45(Pt 1):215–224. doi: 10.1101/sqb.1981.045.01.034. [DOI] [PubMed] [Google Scholar]
  10. Maxam A. M., Gilbert W. Sequencing end-labeled DNA with base-specific chemical cleavages. Methods Enzymol. 1980;65(1):499–560. doi: 10.1016/s0076-6879(80)65059-9. [DOI] [PubMed] [Google Scholar]
  11. Mizuuchi K., Craigie R. Mechanism of bacteriophage mu transposition. Annu Rev Genet. 1986;20:385–429. doi: 10.1146/annurev.ge.20.120186.002125. [DOI] [PubMed] [Google Scholar]
  12. Reyes O., Beyou A., Mignotte-Vieux C., Richaud F. Mini-Mu transduction: cis-inhibition of the insertion of Mud transposons. Plasmid. 1987 Nov;18(3):183–192. doi: 10.1016/0147-619x(87)90061-8. [DOI] [PubMed] [Google Scholar]
  13. Tu C. P., Cohen S. N. Translocation specificity of the Tn3 element: characterization of sites of multiple insertions. Cell. 1980 Jan;19(1):151–160. doi: 10.1016/0092-8674(80)90396-7. [DOI] [PubMed] [Google Scholar]
  14. Vieira J., Messing J. The pUC plasmids, an M13mp7-derived system for insertion mutagenesis and sequencing with synthetic universal primers. Gene. 1982 Oct;19(3):259–268. doi: 10.1016/0378-1119(82)90015-4. [DOI] [PubMed] [Google Scholar]
  15. Waddell C. S., Craig N. L. Tn7 transposition: two transposition pathways directed by five Tn7-encoded genes. Genes Dev. 1988 Feb;2(2):137–149. doi: 10.1101/gad.2.2.137. [DOI] [PubMed] [Google Scholar]
  16. Yanisch-Perron C., Vieira J., Messing J. Improved M13 phage cloning vectors and host strains: nucleotide sequences of the M13mp18 and pUC19 vectors. Gene. 1985;33(1):103–119. doi: 10.1016/0378-1119(85)90120-9. [DOI] [PubMed] [Google Scholar]
  17. Zerbib D., Gamas P., Chandler M., Prentki P., Bass S., Galas D. Specificity of insertion of IS1. J Mol Biol. 1985 Oct 5;185(3):517–524. doi: 10.1016/0022-2836(85)90068-3. [DOI] [PubMed] [Google Scholar]

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