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British Journal of Experimental Pathology logoLink to British Journal of Experimental Pathology
. 1975 Feb;56(1):52–61.

Action of histamine on endothelial cells of guinea-pig isolated hepatic portal vein and its modification by indomethacin or removal of calcium.

A M Northover
PMCID: PMC2072717  PMID: 1106749

Abstract

Methods are described for preparing guinea-pig hepatic portal vein endothelium for light microscopy and electron microscopy. Histamine (100 mug/ml) caused damage to the endothelium which was visible with both the light and electron microscopes. The damage was reduced in the absence of calcium. The reduction was more apparent with the electron microscope than with the light microscope. Indomethacin (100 mug/ml) protected the endothelial cells against the damaging effects of histamine. Possible modes of action of histamine and indomethacin are discussed.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Becker C. G., Murphy G. E. Demonstration of contractile protein in endothelium and cells of the heart valves, endocardium, intima, arteriosclerotic plaques, and Aschoff bodies of rheumatic heart disease. Am J Pathol. 1969 Apr;55(1):1–37. [PMC free article] [PubMed] [Google Scholar]
  2. Björkerud S., Bondjers G. Endothelial integrity and viability in the aorta of the normal rabbit and rat as evaluated with dye exclusion tests and interference contrast microscopy. Atherosclerosis. 1972 May-Jun;15(3):285–300. doi: 10.1016/0021-9150(72)90019-6. [DOI] [PubMed] [Google Scholar]
  3. Cotran R. S., Suter E. R., Majno G. The use of colloidal carbon as a tracer for vascular injury. A review. Vasc Dis. 1967 Apr;4(2):107–127. [PubMed] [Google Scholar]
  4. FLOREY H. W., POOLE J. C., MEEK G. A. Endothelial cells and cement lines. J Pathol Bacteriol. 1959 Apr;77(2):625–636. doi: 10.1002/path.1700770234. [DOI] [PubMed] [Google Scholar]
  5. Garbarsch C., Christensen B. C. Scanning electron microscopy of aortic endothelial cell boundaries after staining with silver nitrate. Angiologica. 1970;7(6):365–373. doi: 10.1159/000157852. [DOI] [PubMed] [Google Scholar]
  6. Gaynor E., Bouvier C., Spaet T. H. Vascular lesions: possible pathogenetic basis of the generalized Shwartzman reaction. Science. 1970 Nov 27;170(3961):986–988. doi: 10.1126/science.170.3961.986. [DOI] [PubMed] [Google Scholar]
  7. HURLEY J. V. An electron microscopic study of leucocytic emigration and vascular permeability in rat skin. Aust J Exp Biol Med Sci. 1963 Apr;41:171–186. doi: 10.1038/icb.1963.17. [DOI] [PubMed] [Google Scholar]
  8. Hoff H. F., Gottlob R. A fine structure study of injury to the endothelial cells of the rabbit abdominal aorta by various stimuli. Angiology. 1967 Jul;18(7):440–451. doi: 10.1177/000331976701800704. [DOI] [PubMed] [Google Scholar]
  9. Hurley J. V., Ryan G. B. A delayed prolonged increase in venular permeability following intrapleural injections in the rat. J Pathol Bacteriol. 1967 Jan;93(1):87–99. doi: 10.1002/path.1700930108. [DOI] [PubMed] [Google Scholar]
  10. Joris I., Majno G., Ryan G. B. Endothelial contraction in vivo: a study of the rat mesentery. Virchows Arch B Cell Pathol. 1972;12(1):73–83. doi: 10.1007/BF02893987. [DOI] [PubMed] [Google Scholar]
  11. MCGOVERN V. J. Mast cells and their relationship to endothelial surfaces. J Pathol Bacteriol. 1956 Jan;71(1):1–6. [PubMed] [Google Scholar]
  12. Majno G., Gilmore V., Leventhal M. On the mechanism of vascular leakage caused by histaminetype mediators. A microscopic study in vivo. Circ Res. 1967 Dec;21(6):833–847. doi: 10.1161/01.res.21.6.833. [DOI] [PubMed] [Google Scholar]
  13. Majno G., Shea S. M., Leventhal M. Endothelial contraction induced by histamine-type mediators: an electron microscopic study. J Cell Biol. 1969 Sep;42(3):647–672. doi: 10.1083/jcb.42.3.647. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Northover A. M., Northover B. J. The effects of histamine, 5-hydroxytryptamine and bradykinin on rat mesenteric blood vessels. J Pathol. 1969 Aug;98(4):265–275. doi: 10.1002/path.1710980406. [DOI] [PubMed] [Google Scholar]
  15. Northover B. J. Effect of anti-inflammatory drugs on the binding of calcium to cellular membranes in various human and guinea-pig tissues. Br J Pharmacol. 1973 Jul;48(3):496–504. doi: 10.1111/j.1476-5381.1973.tb08356.x. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Northover B. J. Mechanism of the inhibitory action of indomethacin on smooth muscle. Br J Pharmacol. 1971 Mar;41(3):540–551. doi: 10.1111/j.1476-5381.1971.tb08052.x. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. POOLE J. C., SANDERS A. G., FLOREY H. W. The regeneration of aortic endothelium. J Pathol Bacteriol. 1958 Jan;75(1):133–143. doi: 10.1002/path.1700750116. [DOI] [PubMed] [Google Scholar]
  18. Peters T. J., Müller M., De Duve C. Lysosomes of the arterial wall. I. Isolation and subcellular fractionation of cells from normal rabbit aorta. J Exp Med. 1972 Nov 1;136(5):1117–1139. doi: 10.1084/jem.136.5.1117. [DOI] [PMC free article] [PubMed] [Google Scholar]
  19. ROBERTSON H. R., MOORE J. R., MERSEREAU W. A. Observations on thrombosis and endothelial repair following application of external pressure to a vein. Can J Surg. 1959 Oct;3:5–16. [PubMed] [Google Scholar]
  20. Tapp R. L., Trowell O. A. The experimental production of watery vacuolation in the acinar cells of the submandibular gland. J Physiol. 1967 Jan;188(2):191–205. doi: 10.1113/jphysiol.1967.sp008133. [DOI] [PMC free article] [PubMed] [Google Scholar]
  21. Tranzer J. P., Baumgartner H. R. Filling gaps in the vascular endothelium with blood platelets. Nature. 1967 Dec 16;216(5120):1126–1128. doi: 10.1038/2161126a0. [DOI] [PubMed] [Google Scholar]

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