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. 1992 Nov;174(21):7059–7062. doi: 10.1128/jb.174.21.7059-7062.1992

Conditional growth of Escherichia coli caused by expression of vaccinia virus DNA topoisomerase I.

M E Fernandez-Beros 1, Y C Tse-Dinh 1
PMCID: PMC207389  PMID: 1328167

Abstract

Active vaccinia virus topoisomerase I is expressed in Escherichia coli containing plasmid p1940 (S. Shuman, M. Golder, and B. Moss, J. Biol. Chem. 263:16401-16407, 1988). Growth curves showed a decline of 2 to 3 logs in the number of viable cells at 42 degrees C after shift from 30 degrees C because of increased vaccinia virus topoisomerase I level. Mutations in the gyrA and gyrB genes allowed cells to grow equally well at 42 and 30 degrees C. The presence of gyrase inhibitor also improved growth at 42 degrees C.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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