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. 1996 Nov;74(9):1419–1422. doi: 10.1038/bjc.1996.558

Expression of a photoreceptor protein, recoverin, as a cancer-associated retinopathy autoantigen in human lung cancer cell lines.

S Matsubara 1, Y Yamaji 1, M Sato 1, J Fujita 1, J Takahara 1
PMCID: PMC2074779  PMID: 8912538

Abstract

Recently, a photoreceptor protein, recoverin, has been recognised as an autoantigen of cancer-associated retinopathy (CAR), a rare paraneoplastic neurological syndrome often associated with patients with small-cell lung cancer (SCLC). Although until quite recently the specific expression of recoverin in cancer cells had not been indicated, Polans et al. (Polans AS, Witkowska D, Haley TL, Amundson D, Baizer L, Adamus G 1995, Proc. Natl. Acad. Sci. USA, 92, 9176-9180) demonstrated the specific expression of recoverin in lung tumour and primary cultured tumour cells from a CAR patient. We examined the expression of recoverin in human lung cancer cell lines by reverse transcription polymerase chain reaction (PCR), Northern blotting and Western immunoblotting. Recoverin was expressed in only one SCLC cell line from a patient with CAR. The sequence of recoverin cDNA from the cells was identical to the human recoverin sequence. These findings strongly support the hypothesis that the ectopic expression of wild-type recoverin in SCLC induces the cancer-retina immunological cross-reaction, leading to visual loss in CAR.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Adamus G., Ortega H., Witkowska D., Polans A. Recoverin: a potent uveitogen for the induction of photoreceptor degeneration in Lewis rats. Exp Eye Res. 1994 Oct;59(4):447–455. doi: 10.1006/exer.1994.1130. [DOI] [PubMed] [Google Scholar]
  2. Dalmau J., Furneaux H. M., Cordon-Cardo C., Posner J. B. The expression of the Hu (paraneoplastic encephalomyelitis/sensory neuronopathy) antigen in human normal and tumor tissues. Am J Pathol. 1992 Oct;141(4):881–886. [PMC free article] [PubMed] [Google Scholar]
  3. Fujita T., Yamaji Y., Sato M., Murao K., Takahara J. Gene expression of somatostatin receptor subtypes, SSTR1 and SSTR2, in human lung cancer cell lines. Life Sci. 1994;55(23):1797–1806. doi: 10.1016/0024-3205(94)90090-6. [DOI] [PubMed] [Google Scholar]
  4. Furneaux H. M., Rosenblum M. K., Dalmau J., Wong E., Woodruff P., Graus F., Posner J. B. Selective expression of Purkinje-cell antigens in tumor tissue from patients with paraneoplastic cerebellar degeneration. N Engl J Med. 1990 Jun 28;322(26):1844–1851. doi: 10.1056/NEJM199006283222604. [DOI] [PubMed] [Google Scholar]
  5. Gery I., Chanaud N. P., 3rd, Anglade E. Recoverin is highly uveitogenic in Lewis rats. Invest Ophthalmol Vis Sci. 1994 Jul;35(8):3342–3345. [PubMed] [Google Scholar]
  6. Kawamura S. Photoreceptor light-adaptation mediated by S-modulin, a member of a possible regulatory protein family of protein phosphorylation in signal transduction. Neurosci Res. 1994 Oct;20(4):293–298. doi: 10.1016/0168-0102(94)90050-7. [DOI] [PubMed] [Google Scholar]
  7. Kornguth S. E. Neuronal proteins and paraneoplastic syndromes. N Engl J Med. 1989 Dec 7;321(23):1607–1608. doi: 10.1056/NEJM198912073212310. [DOI] [PubMed] [Google Scholar]
  8. Matsubara S., Sato M., Mizobuchi M., Niimi M., Takahara J. Differential gene expression of growth hormone (GH)-releasing hormone (GRH) and GRH receptor in various rat tissues. Endocrinology. 1995 Sep;136(9):4147–4150. doi: 10.1210/endo.136.9.7649123. [DOI] [PubMed] [Google Scholar]
  9. Murakami A., Yajima T., Inana G. Isolation of human retinal genes: recoverin cDNA and gene. Biochem Biophys Res Commun. 1992 Aug 31;187(1):234–244. doi: 10.1016/s0006-291x(05)81483-4. [DOI] [PubMed] [Google Scholar]
  10. Oguro-Okano M., Griesmann G. E., Wieben E. D., Slaymaker S. J., Snutch T. P., Lennon V. A. Molecular diversity of neuronal-type calcium channels identified in small cell lung carcinoma. Mayo Clin Proc. 1992 Dec;67(12):1150–1159. doi: 10.1016/s0025-6196(12)61144-6. [DOI] [PubMed] [Google Scholar]
  11. Polans A. S., Buczyłko J., Crabb J., Palczewski K. A photoreceptor calcium binding protein is recognized by autoantibodies obtained from patients with cancer-associated retinopathy. J Cell Biol. 1991 Mar;112(5):981–989. doi: 10.1083/jcb.112.5.981. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Polans A. S., Witkowska D., Haley T. L., Amundson D., Baizer L., Adamus G. Recoverin, a photoreceptor-specific calcium-binding protein, is expressed by the tumor of a patient with cancer-associated retinopathy. Proc Natl Acad Sci U S A. 1995 Sep 26;92(20):9176–9180. doi: 10.1073/pnas.92.20.9176. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Polans A. S., Witkowska D., Haley T. L., Amundson D., Baizer L., Adamus G. Recoverin, a photoreceptor-specific calcium-binding protein, is expressed by the tumor of a patient with cancer-associated retinopathy. Proc Natl Acad Sci U S A. 1995 Sep 26;92(20):9176–9180. doi: 10.1073/pnas.92.20.9176. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Sato M., Frohman L. A. Differential effects of central and peripheral administration of growth hormone (GH) and insulin-like growth factor on hypothalamic GH-releasing hormone and somatostatin gene expression in GH-deficient dwarf rats. Endocrinology. 1993 Aug;133(2):793–799. doi: 10.1210/endo.133.2.8102097. [DOI] [PubMed] [Google Scholar]
  15. Sekido Y., Bader S. A., Carbone D. P., Johnson B. E., Minna J. D. Molecular analysis of the HuD gene encoding a paraneoplastic encephalomyelitis antigen in human lung cancer cell lines. Cancer Res. 1994 Sep 15;54(18):4988–4992. [PubMed] [Google Scholar]
  16. Thirkill C. E., FitzGerald P., Sergott R. C., Roth A. M., Tyler N. K., Keltner J. L. Cancer-associated retinopathy (CAR syndrome) with antibodies reacting with retinal, optic-nerve, and cancer cells. N Engl J Med. 1989 Dec 7;321(23):1589–1594. doi: 10.1056/NEJM198912073212307. [DOI] [PubMed] [Google Scholar]
  17. Thirkill C. E., Keltner J. L., Tyler N. K., Roth A. M. Antibody reactions with retina and cancer-associated antigens in 10 patients with cancer-associated retinopathy. Arch Ophthalmol. 1993 Jul;111(7):931–937. doi: 10.1001/archopht.1993.01090070049018. [DOI] [PubMed] [Google Scholar]
  18. Thirkill C. E., Tait R. C., Tyler N. K., Roth A. M., Keltner J. L. The cancer-associated retinopathy antigen is a recoverin-like protein. Invest Ophthalmol Vis Sci. 1992 Sep;33(10):2768–2772. [PubMed] [Google Scholar]
  19. Yamaji Y., Matsubara S., Yamadori I., Sato M., Fujita T., Fujita J., Takahara J. Characterization of a small-cell-lung-carcinoma cell line from a patient with cancer-associated retinopathy. Int J Cancer. 1996 Mar 1;65(5):671–676. doi: 10.1002/(SICI)1097-0215(19960301)65:5<671::AID-IJC18>3.0.CO;2-A. [DOI] [PubMed] [Google Scholar]

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