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. 1992 Dec;174(24):7963–7970. doi: 10.1128/jb.174.24.7963-7970.1992

Structures of the O1B and O1C lipopolysaccharide antigens of Escherichia coli.

D S Gupta 1, A S Shashkov 1, B Jann 1, K Jann 1
PMCID: PMC207532  PMID: 1281148

Abstract

The O-specific moieties of the O1B antigen (lipopolysaccharide) from Escherichia coli O1B:K1 and the O1C antigen from E. coli O1C:K- both consist of L-rhamnose, D-galactose, N-acetyl-D-glucosamine, and N-acetyl-D-mannosamine in a molar ratio of 2:1:1:1. By using fragmentation procedures, methylation analysis, and one- and two-dimensional nuclear magnetic resonance spectroscopy, the structures of these polysaccharides were found to be [formula: see text] In the O1B polysaccharide X is 2, and in the O1C polysaccharide X is 3. With the recently published structure of the O1A polysaccharides (B. Jann, A. S. Shashkov, D. S. Gupta, S. M. Panasenko, and K. Jann, Carbohydr. Polym. 18:51-57 1992), three related O1 antigens are now known. Their common (O1-specific) epitope is suggested to be the side-chain N-acetyl-D-mannosamine residue.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Achtman M., Mercer A., Kusecek B., Pohl A., Heuzenroeder M., Aaronson W., Sutton A., Silver R. P. Six widespread bacterial clones among Escherichia coli K1 isolates. Infect Immun. 1983 Jan;39(1):315–335. doi: 10.1128/iai.39.1.315-335.1983. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Cross A. S. The biologic significance of bacterial encapsulation. Curr Top Microbiol Immunol. 1990;150:87–95. doi: 10.1007/978-3-642-74694-9_5. [DOI] [PubMed] [Google Scholar]
  3. Gupta D. S., Jann B., Jann K. Escherichia coli O18ac antigen: structure of the O-specific polysaccharide moiety. Infect Immun. 1984 Jul;45(1):203–209. doi: 10.1128/iai.45.1.203-209.1984. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Joiner K. A., Brown E. J., Frank M. M. Complement and bacteria: chemistry and biology in host defense. Annu Rev Immunol. 1984;2:461–491. doi: 10.1146/annurev.iy.02.040184.002333. [DOI] [PubMed] [Google Scholar]
  5. Kenne L., Lindberg B., Petersson K., Katzenellenbogen E., Romanowska E. Structural studies of Shigella flexneri O-antigens. Eur J Biochem. 1978 Nov 2;91(1):279–284. doi: 10.1111/j.1432-1033.1978.tb20963.x. [DOI] [PubMed] [Google Scholar]
  6. Kocharova N. A., Knirel Y. A., Shashkov A. S., Kochetkov N. K., Pier G. B. Structure of an extracellular cross-reactive polysaccharide from Pseudomonas aeruginosa immunotype 4. J Biol Chem. 1988 Aug 15;263(23):11291–11295. [PubMed] [Google Scholar]
  7. Kusecek B., Wloch H., Mercer A., Vaisänen V., Pluschke G., Korhonen T., Achtman M. Lipopolysaccharide, capsule, and fimbriae as virulence factors among O1, O7, O16, O18, or O75 and K1, K5, or K100 Escherichia coli. Infect Immun. 1984 Jan;43(1):368–379. doi: 10.1128/iai.43.1.368-379.1984. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Moll A., Kusecek B., Pluschke G., Morelli G., Kamke M., Jann B., Jann K., Achtman M. A reexamination of the O1 lipopolysaccharide antigen group of Escherichia coli. Infect Immun. 1986 Aug;53(2):257–263. doi: 10.1128/iai.53.2.257-263.1986. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Myerowitz R. L., Albers A. C., Yee R. B., Orskov F. Relationship of K1 antigen to biotype in clinical isolates of Escherichia coli. J Clin Microbiol. 1977 Aug;6(2):124–127. doi: 10.1128/jcm.6.2.124-127.1977. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Niedermeier W., Tomana M. Gas chromatographic analysis of hexosamines in glycoproteins. Anal Biochem. 1974 Feb;57(2):363–368. doi: 10.1016/0003-2697(74)90090-6. [DOI] [PubMed] [Google Scholar]
  11. Nimmich W., Zingler G. Biochemical characteristics, phage patterns, and O1 factor analysis of Escherichia coli O1:K1:H7:F11 and O1:K1:H-:F9 strains isolated from patients with urinary tract infections. Med Microbiol Immunol. 1984;173(2):75–85. doi: 10.1007/BF02124821. [DOI] [PubMed] [Google Scholar]
  12. Orskov F., Orskov I. Complex formation between Escherichia coli lipopolysaccharide O antigen and capsular K antigen as detected by immunoelectrophoresis. APMIS. 1991 Jul;99(7):615–619. [PubMed] [Google Scholar]
  13. Orskov I., Orskov F., Jann B., Jann K. Serology, chemistry, and genetics of O and K antigens of Escherichia coli. Bacteriol Rev. 1977 Sep;41(3):667–710. doi: 10.1128/br.41.3.667-710.1977. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Pluschke G., Mayden J., Achtman M., Levine R. P. Role of the capsule and the O antigen in resistance of O18:K1 Escherichia coli to complement-mediated killing. Infect Immun. 1983 Dec;42(3):907–913. doi: 10.1128/iai.42.3.907-913.1983. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Robbins J. B., McCracken G. H., Jr, Gotschlich E. C., Orskov F., Orskov I., Hanson L. A. Escherichia coli K1 capsular polysaccharide associated with neonatal meningitis. N Engl J Med. 1974 May 30;290(22):1216–1220. doi: 10.1056/NEJM197405302902202. [DOI] [PubMed] [Google Scholar]
  16. Stellner K., Saito H., Hakomori S. I. Determination of aminosugar linkages in glycolipids by methylation. Aminosugar linkages of ceramide pentasaccharides of rabbit erythrocytes and of Forssman antigen. Arch Biochem Biophys. 1973 Apr;155(2):464–472. doi: 10.1016/0003-9861(73)90138-0. [DOI] [PubMed] [Google Scholar]
  17. Vermeulen C., Cross A., Byrne W. R., Zollinger W. Quantitative relationship between capsular content and killing of K1-encapsulated Escherichia coli. Infect Immun. 1988 Oct;56(10):2723–2730. doi: 10.1128/iai.56.10.2723-2730.1988. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. WILSON D. M., AJL S. Metabolism of L-rhamnose by Escherichia coli. I. L-rhamnose isomerase. J Bacteriol. 1957 Mar;73(3):410–414. doi: 10.1128/jb.73.3.410-414.1957. [DOI] [PMC free article] [PubMed] [Google Scholar]

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