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. 1992 Dec;174(24):8172–8177. doi: 10.1128/jb.174.24.8172-8177.1992

Evidence that the hemolysin/bacteriocin phenotype of Enterococcus faecalis subsp. zymogenes can be determined by plasmids in different incompatibility groups as well as by the chromosome.

Y Ike 1, D B Clewell 1
PMCID: PMC207561  PMID: 1459967

Abstract

The hemolysin (Hly/Bac) determinant in strains of Enterococcus faecalis was found to be present on plasmids in different incompatibility groups (conferring different sex pheromone responses) as well as on the chromosome. Of 33 Hly/Bac plasmids identified in clinical isolates, the related pheromone for 30 was cAD1; the related pheromone for another two (pYI1 and pYI3) or one (pYI2) was cOB1 or cY12, respectively. The representative Hly/Bac plasmids pAD1, pYI1, pOB1, and pYI2, which responded to pheromones cAD1, cOB1, cOB1, and cYI2, respectively, were compatible with one another. As additions to the incompatibility group IncHly of pAD1, groups for pOB1, pYI1, and pYI2 were designated IncHlyII, IncHlyIII, and IncHlyIV, respectively. Eleven of the 30 plasmids conferring a response to cAD1 were very similar to pAD1 on the basis of their restriction endonuclease profiles. EcoRI fragment D, F, or H containing parts of the Hly/Bac gene(s) of pAD1 hybridized to similar EcoRI fragments from each of the other three representatives of incompatibility groups (i.e., pOB1, pYI1, and pYI2) and to homologous DNA representing the chromosome of the plasmid-free Hly/Bac strain YI6-1.

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Selected References

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  1. BROCK T. D., DAVIE J. M. PROBABLE IDENTITY OF A GROUP D HEMOLYSIN WITH A BACTERIOCINE. J Bacteriol. 1963 Oct;86:708–712. doi: 10.1128/jb.86.4.708-712.1963. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Basinger S. F., Jackson R. W. Bacteriocin (hemolysin) of Streptococcus zymogenes. J Bacteriol. 1968 Dec;96(6):1895–1902. doi: 10.1128/jb.96.6.1895-1902.1968. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Clewell D. B., An F. Y., Mori M., Ike Y., Suzuki A. Streptococcus faecalis sex pheromone (cAD1) response: evidence that the peptide inhibitor excreted by pAD1-containing cells may be plasmid determined. Plasmid. 1987 Jan;17(1):65–68. doi: 10.1016/0147-619x(87)90011-4. [DOI] [PubMed] [Google Scholar]
  4. Clewell D. B., Brown B. L. Sex pheromone cAD1 in Streptococcus faecalis: induction of a function related to plasmid transfer. J Bacteriol. 1980 Aug;143(2):1063–1065. doi: 10.1128/jb.143.2.1063-1065.1980. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Clewell D. B., Flannagan S. E., Ike Y., Jones J. M., Gawron-Burke C. Sequence analysis of termini of conjugative transposon Tn916. J Bacteriol. 1988 Jul;170(7):3046–3052. doi: 10.1128/jb.170.7.3046-3052.1988. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Clewell D. B. Plasmids, drug resistance, and gene transfer in the genus Streptococcus. Microbiol Rev. 1981 Sep;45(3):409–436. doi: 10.1128/mr.45.3.409-436.1981. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Clewell D. B., Pontius L. T., An F. Y., Ike Y., Suzuki A., Nakayama J. Nucleotide sequence of the sex pheromone inhibitor (iAD1) determinant of Enterococcus faecalis conjugative plasmid pAD1. Plasmid. 1990 Sep;24(2):156–161. doi: 10.1016/0147-619x(90)90019-9. [DOI] [PubMed] [Google Scholar]
  8. Clewell D. B., Tomich P. K., Gawron-Burke M. C., Franke A. E., Yagi Y., An F. Y. Mapping of Streptococcus faecalis plasmids pAD1 and pAD2 and studies relating to transposition of Tn917. J Bacteriol. 1982 Dec;152(3):1220–1230. doi: 10.1128/jb.152.3.1220-1230.1982. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Clewell D. B., Weaver K. E. Sex pheromones and plasmid transfer in Enterococcus faecalis. Plasmid. 1989 May;21(3):175–184. doi: 10.1016/0147-619x(89)90041-3. [DOI] [PubMed] [Google Scholar]
  10. Colmar I., Horaud T. Enterococcus faecalis hemolysin-bacteriocin plasmids belong to the same incompatibility group. Appl Environ Microbiol. 1987 Mar;53(3):567–570. doi: 10.1128/aem.53.3.567-570.1987. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Dunny G. M., Brown B. L., Clewell D. B. Induced cell aggregation and mating in Streptococcus faecalis: evidence for a bacterial sex pheromone. Proc Natl Acad Sci U S A. 1978 Jul;75(7):3479–3483. doi: 10.1073/pnas.75.7.3479. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Dunny G. M., Clewell D. B. Transmissible toxin (hemolysin) plasmid in Streptococcus faecalis and its mobilization of a noninfectious drug resistance plasmid. J Bacteriol. 1975 Nov;124(2):784–790. doi: 10.1128/jb.124.2.784-790.1975. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Dunny G. M., Craig R. A., Carron R. L., Clewell D. B. Plasmid transfer in Streptococcus faecalis: production of multiple sex pheromones by recipients. Plasmid. 1979 Jul;2(3):454–465. doi: 10.1016/0147-619x(79)90029-5. [DOI] [PubMed] [Google Scholar]
  14. Ehrenfeld E. E., Clewell D. B. Transfer functions of the Streptococcus faecalis plasmid pAD1: organization of plasmid DNA encoding response to sex pheromone. J Bacteriol. 1987 Aug;169(8):3473–3481. doi: 10.1128/jb.169.8.3473-3481.1987. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Franke A. E., Clewell D. B. Evidence for a chromosome-borne resistance transposon (Tn916) in Streptococcus faecalis that is capable of "conjugal" transfer in the absence of a conjugative plasmid. J Bacteriol. 1981 Jan;145(1):494–502. doi: 10.1128/jb.145.1.494-502.1981. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Franke A. E., Clewell D. B. Evidence for conjugal transfer of a Streptococcus faecalis transposon (Tn916) from a chromosomal site in the absence of plasmid DNA. Cold Spring Harb Symp Quant Biol. 1981;45(Pt 1):77–80. doi: 10.1101/sqb.1981.045.01.014. [DOI] [PubMed] [Google Scholar]
  17. Fujimoto S., Hashimoto H., Ike Y. Low cost device for electrotransformation and its application to the highly efficient transformation of Escherichia coli and Enterococcus faecalis. Plasmid. 1991 Sep;26(2):131–135. doi: 10.1016/0147-619x(91)90053-y. [DOI] [PubMed] [Google Scholar]
  18. Galli D., Lottspeich F., Wirth R. Sequence analysis of Enterococcus faecalis aggregation substance encoded by the sex pheromone plasmid pAD1. Mol Microbiol. 1990 Jun;4(6):895–904. doi: 10.1111/j.1365-2958.1990.tb00662.x. [DOI] [PubMed] [Google Scholar]
  19. Gawron-Burke C., Clewell D. B. Regeneration of insertionally inactivated streptococcal DNA fragments after excision of transposon Tn916 in Escherichia coli: strategy for targeting and cloning of genes from gram-positive bacteria. J Bacteriol. 1984 Jul;159(1):214–221. doi: 10.1128/jb.159.1.214-221.1984. [DOI] [PMC free article] [PubMed] [Google Scholar]
  20. Ike Y., Clewell D. B. Genetic analysis of the pAD1 pheromone response in Streptococcus faecalis, using transposon Tn917 as an insertional mutagen. J Bacteriol. 1984 Jun;158(3):777–783. doi: 10.1128/jb.158.3.777-783.1984. [DOI] [PMC free article] [PubMed] [Google Scholar]
  21. Ike Y., Clewell D. B., Segarra R. A., Gilmore M. S. Genetic analysis of the pAD1 hemolysin/bacteriocin determinant in Enterococcus faecalis: Tn917 insertional mutagenesis and cloning. J Bacteriol. 1990 Jan;172(1):155–163. doi: 10.1128/jb.172.1.155-163.1990. [DOI] [PMC free article] [PubMed] [Google Scholar]
  22. Ike Y., Craig R. A., White B. A., Yagi Y., Clewell D. B. Modification of Streptococcus faecalis sex pheromones after acquisition of plasmid DNA. Proc Natl Acad Sci U S A. 1983 Sep;80(17):5369–5373. doi: 10.1073/pnas.80.17.5369. [DOI] [PMC free article] [PubMed] [Google Scholar]
  23. Ike Y., Flannagan S. E., Clewell D. B. Hyperhemolytic phenomena associated with insertions of Tn916 into the hemolysin determinant of Enterococcus faecalis plasmid pAD1. J Bacteriol. 1992 Mar;174(6):1801–1809. doi: 10.1128/jb.174.6.1801-1809.1992. [DOI] [PMC free article] [PubMed] [Google Scholar]
  24. Ike Y., Hashimoto H., Clewell D. B. Hemolysin of Streptococcus faecalis subspecies zymogenes contributes to virulence in mice. Infect Immun. 1984 Aug;45(2):528–530. doi: 10.1128/iai.45.2.528-530.1984. [DOI] [PMC free article] [PubMed] [Google Scholar]
  25. Ike Y., Hashimoto H., Clewell D. B. High incidence of hemolysin production by Enterococcus (Streptococcus) faecalis strains associated with human parenteral infections. J Clin Microbiol. 1987 Aug;25(8):1524–1528. doi: 10.1128/jcm.25.8.1524-1528.1987. [DOI] [PMC free article] [PubMed] [Google Scholar]
  26. Ike Y., Hashimoto H., Mitsuhashi S. A mutant defective in partitioning of composite plasmid Rms201. J Bacteriol. 1981 Aug;147(2):578–588. doi: 10.1128/jb.147.2.578-588.1981. [DOI] [PMC free article] [PubMed] [Google Scholar]
  27. Jacob A. E., Douglas G. J., Hobbs S. J. Self-transferable plasmids determining the hemolysin and bacteriocin of Streptococcus faecalis var. zymogenes. J Bacteriol. 1975 Mar;121(3):863–872. doi: 10.1128/jb.121.3.863-872.1975. [DOI] [PMC free article] [PubMed] [Google Scholar]
  28. Kreft B., Marre R., Schramm U., Wirth R. Aggregation substance of Enterococcus faecalis mediates adhesion to cultured renal tubular cells. Infect Immun. 1992 Jan;60(1):25–30. doi: 10.1128/iai.60.1.25-30.1992. [DOI] [PMC free article] [PubMed] [Google Scholar]
  29. LeBlanc D. J., Lee L. N., Clewell D. B., Behnke D. Broad geographical distribution of a cytotoxin gene mediating beta-hemolysis and bacteriocin activity among Streptococcus faecalis strains. Infect Immun. 1983 Jun;40(3):1015–1022. doi: 10.1128/iai.40.3.1015-1022.1983. [DOI] [PMC free article] [PubMed] [Google Scholar]
  30. Oliver D. R., Brown B. L., Clewell D. B. Analysis of plasmid deoxyribonucleic acid in a cariogenic strain of Streptococcus faecalis: an approach to identifying genetic determinants on cryptic plasmids. J Bacteriol. 1977 May;130(2):759–765. doi: 10.1128/jb.130.2.759-765.1977. [DOI] [PMC free article] [PubMed] [Google Scholar]
  31. Pontius L. T., Clewell D. B. A phase variation event that activates conjugation functions encoded by the Enterococcus faecalis plasmid pAD1. Plasmid. 1991 Nov;26(3):172–185. doi: 10.1016/0147-619x(91)90041-t. [DOI] [PubMed] [Google Scholar]
  32. Tomich P. K., An F. Y., Clewell D. B. Properties of erythromycin-inducible transposon Tn917 in Streptococcus faecalis. J Bacteriol. 1980 Mar;141(3):1366–1374. doi: 10.1128/jb.141.3.1366-1374.1980. [DOI] [PMC free article] [PubMed] [Google Scholar]
  33. Tomich P. K., An F. Y., Damle S. P., Clewell D. B. Plasmid-related transmissibility and multiple drug resistance in Streptococcus faecalis subsp. zymogenes strain DS16. Antimicrob Agents Chemother. 1979 Jun;15(6):828–830. doi: 10.1128/aac.15.6.828. [DOI] [PMC free article] [PubMed] [Google Scholar]
  34. Yagi Y., Clewell D. B. Recombination-deficient mutant of Streptococcus faecalis. J Bacteriol. 1980 Aug;143(2):966–970. doi: 10.1128/jb.143.2.966-970.1980. [DOI] [PMC free article] [PubMed] [Google Scholar]

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