Skip to main content
PMC home page
Journal of Bacteriology logoLink to Journal of Bacteriology
. 1992 Oct;174(20):6432–6437. doi: 10.1128/jb.174.20.6432-6437.1992

An alpha-helical hydrophobic hairpin as a specific determinant in protein-protein interaction occurring in Escherichia coli colicin A and B immunity systems.

V Geli 1, C Lazdunski 1
PMCID: PMC207597  PMID: 1400195

Abstract

A collection of chimeric pore-forming domains between colicins A and B was constructed to investigate the specific determinants responsible for recognition by the corresponding immunity proteins. The fusion sites in the hybrid proteins were positioned according to the three-dimensional structure of the soluble form of the colicin A pore-forming domain. The hydrophobic hairpin of colicin pore-forming domains, buried in the core of the soluble structure, was the main determinant recognized by the integral immunity proteins. The immunity protein function may require helix-helix recognition within the lipid bilayer.

Full text

PDF
6432

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Baty D., Frenette M., Lloubès R., Geli V., Howard S. P., Pattus F., Lazdunski C. Functional domains of colicin A. Mol Microbiol. 1988 Nov;2(6):807–811. doi: 10.1111/j.1365-2958.1988.tb00092.x. [DOI] [PubMed] [Google Scholar]
  2. Baty D., Knibiehler M., Verheij H., Pattus F., Shire D., Bernadac A., Lazdunski C. Site-directed mutagenesis of the COOH-terminal region of colicin A: effect on secretion and voltage-dependent channel activity. Proc Natl Acad Sci U S A. 1987 Mar;84(5):1152–1156. doi: 10.1073/pnas.84.5.1152. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Benedetti H., Frenette M., Baty D., Knibiehler M., Pattus F., Lazdunski C. Individual domains of colicins confer specificity in colicin uptake, in pore-properties and in immunity requirement. J Mol Biol. 1991 Feb 5;217(3):429–439. doi: 10.1016/0022-2836(91)90747-t. [DOI] [PubMed] [Google Scholar]
  4. Benedetti H., Lazdunski C., Lloubès R. Protein import into Escherichia coli: colicins A and E1 interact with a component of their translocation system. EMBO J. 1991 Aug;10(8):1989–1995. doi: 10.1002/j.1460-2075.1991.tb07728.x. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Chothia C., Lesk A. M. The relation between the divergence of sequence and structure in proteins. EMBO J. 1986 Apr;5(4):823–826. doi: 10.1002/j.1460-2075.1986.tb04288.x. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Cramer W. A., Cohen F. S., Merrill A. R., Song H. Y. Structure and dynamics of the colicin E1 channel. Mol Microbiol. 1990 Apr;4(4):519–526. doi: 10.1111/j.1365-2958.1990.tb00619.x. [DOI] [PubMed] [Google Scholar]
  7. Eick-Helmerich K., Braun V. Import of biopolymers into Escherichia coli: nucleotide sequences of the exbB and exbD genes are homologous to those of the tolQ and tolR genes, respectively. J Bacteriol. 1989 Sep;171(9):5117–5126. doi: 10.1128/jb.171.9.5117-5126.1989. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Geli V., Baty D., Crozel V., Morlon J., Lloubes R., Pattus F., Lazdunski C. A molecular genetic approach to the functioning of the immunity protein to colicin A. Mol Gen Genet. 1986 Mar;202(3):455–460. doi: 10.1007/BF00333276. [DOI] [PubMed] [Google Scholar]
  9. Geli V., Baty D., Lazdunski C. Use of a foreign epitope as a "tag" for the localization of minor proteins within a cell: the case of the immunity protein to colicin A. Proc Natl Acad Sci U S A. 1988 Feb;85(3):689–693. doi: 10.1073/pnas.85.3.689. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Geli V., Baty D., Pattus F., Lazdunski C. Topology and function of the integral membrane protein conferring immunity to colicin A. Mol Microbiol. 1989 May;3(5):679–687. doi: 10.1111/j.1365-2958.1989.tb00216.x. [DOI] [PubMed] [Google Scholar]
  11. Lakey J. H., Baty D., Pattus F. Fluorescence energy transfer distance measurements using site-directed single cysteine mutants. The membrane insertion of colicin A. J Mol Biol. 1991 Apr 5;218(3):639–653. doi: 10.1016/0022-2836(91)90707-d. [DOI] [PubMed] [Google Scholar]
  12. Lakey J. H., González-Mañas J. M., van der Goot F. G., Pattus F. The membrane insertion of colicins. FEBS Lett. 1992 Jul 27;307(1):26–29. doi: 10.1016/0014-5793(92)80895-n. [DOI] [PubMed] [Google Scholar]
  13. Lakey J. H., Massotte D., Heitz F., Dasseux J. L., Faucon J. F., Parker M. W., Pattus F. Membrane insertion of the pore-forming domain of colicin A. A spectroscopic study. Eur J Biochem. 1991 Mar 28;196(3):599–607. doi: 10.1111/j.1432-1033.1991.tb15855.x. [DOI] [PubMed] [Google Scholar]
  14. Lloubes R., Baty D., Lazdunski C. The promoters of the genes for colicin production, release and immunity in the ColA plasmid: effects of convergent transcription and Lex A protein. Nucleic Acids Res. 1986 Mar 25;14(6):2621–2636. doi: 10.1093/nar/14.6.2621. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Mende J., Braun V. Import-defective colicin B derivatives mutated in the TonB box. Mol Microbiol. 1990 Sep;4(9):1523–1533. doi: 10.1111/j.1365-2958.1990.tb02063.x. [DOI] [PubMed] [Google Scholar]
  16. Merrill A. R., Cramer W. A. Identification of a voltage-responsive segment of the potential-gated colicin E1 ion channel. Biochemistry. 1990 Sep 18;29(37):8529–8534. doi: 10.1021/bi00489a004. [DOI] [PubMed] [Google Scholar]
  17. Nogami T., Mizuno T., Mizushima S. Construction of a series of ompF-ompC chimeric genes by in vivo homologous recombination in Escherichia coli and characterization of the translational products. J Bacteriol. 1985 Nov;164(2):797–801. doi: 10.1128/jb.164.2.797-801.1985. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. Pressler U., Braun V., Wittmann-Liebold B., Benz R. Structural and functional properties of colicin B. J Biol Chem. 1986 Feb 25;261(6):2654–2659. [PubMed] [Google Scholar]
  19. Schein S. J., Kagan B. L., Finkelstein A. Colicin K acts by forming voltage-dependent channels in phospholipid bilayer membranes. Nature. 1978 Nov 9;276(5684):159–163. doi: 10.1038/276159a0. [DOI] [PubMed] [Google Scholar]
  20. Schramm E., Mende J., Braun V., Kamp R. M. Nucleotide sequence of the colicin B activity gene cba: consensus pentapeptide among TonB-dependent colicins and receptors. J Bacteriol. 1987 Jul;169(7):3350–3357. doi: 10.1128/jb.169.7.3350-3357.1987. [DOI] [PMC free article] [PubMed] [Google Scholar]
  21. Schramm E., Olschläger T., Tröger W., Braun V. Sequence, expression and localization of the immunity protein for colicin B. Mol Gen Genet. 1988 Jan;211(1):176–182. doi: 10.1007/BF00338410. [DOI] [PubMed] [Google Scholar]
  22. Song H. Y., Cohen F. S., Cramer W. A. Membrane topography of ColE1 gene products: the hydrophobic anchor of the colicin E1 channel is a helical hairpin. J Bacteriol. 1991 May;173(9):2927–2934. doi: 10.1128/jb.173.9.2927-2934.1991. [DOI] [PMC free article] [PubMed] [Google Scholar]
  23. Song H. Y., Cramer W. A. Membrane topography of ColE1 gene products: the immunity protein. J Bacteriol. 1991 May;173(9):2935–2943. doi: 10.1128/jb.173.9.2935-2943.1991. [DOI] [PMC free article] [PubMed] [Google Scholar]
  24. Tommassen J., van der Ley P., van Zeijl M., Agterberg M. Localization of functional domains in E. coli K-12 outer membrane porins. EMBO J. 1985 Jun;4(6):1583–1587. doi: 10.1002/j.1460-2075.1985.tb03820.x. [DOI] [PMC free article] [PubMed] [Google Scholar]
  25. van der Goot F. G., González-Mañas J. M., Lakey J. H., Pattus F. A 'molten-globule' membrane-insertion intermediate of the pore-forming domain of colicin A. Nature. 1991 Dec 5;354(6352):408–410. doi: 10.1038/354408a0. [DOI] [PubMed] [Google Scholar]

Articles from Journal of Bacteriology are provided here courtesy of American Society for Microbiology (ASM)

RESOURCES