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. 1992 Oct;174(20):6674–6677. doi: 10.1128/jb.174.20.6674-6677.1992

Site-specific integration of the Haemophilus influenzae bacteriophage HP1: location of the boundaries of the phage attachment site.

M A Hauser 1, J J Scocca 1
PMCID: PMC207650  PMID: 1383194

Abstract

Plasmids containing DNA segments from the attachment region of phage HP1 were constructed and tested for the ability to replace the phage attachment site substrate in site-specific recombination reactions. The distance separating the boundaries of the functional site was 418 bp. Replacements within the 11-residue segment 5'-GGCGGTTATCG at the left boundary or within the 12-residue segment 5'-GGATTTTTTGAA at the right boundary abolished substrate activity. A segment of the 418-residue sequence preserves the integrity of an operon of three Haemophilus influenzae tRNA genes after HP1 insertion within the coding sequence.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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