Skip to main content
PMC home page
Journal of Bacteriology logoLink to Journal of Bacteriology
. 1991 Apr;173(7):2283–2288. doi: 10.1128/jb.173.7.2283-2288.1991

Hydrolysis and reduction of factor 390 by cell extracts of Methanobacterium thermoautotrophicum (strain delta H).

S W Kengen 1, H W von den Hoff 1, J T Keltjens 1, C van der Drift 1, G D Vogels 1
PMCID: PMC207780  PMID: 2007552

Abstract

Cell extracts of Methanobacterium thermoautotrophicum (strain delta H) were found to perform a hydrogen-dependent reduction of factor 390 (F390), the 8-adenylyl derivative of coenzyme F420. Upon resolution of cell extracts, F390-reducing activity copurified with the coenzyme F420-dependent hydrogenase. This indicates that F390 serves as a substrate of that enzyme. Activity towards F390 was approximately 40-fold lower than that towards coenzyme F420 (0.12 and 5.2 mumol.min-1.mg of protein-1, respectively). In addition, cell extracts catalyzed the hydrolysis of F390 to AMP and coenzyme F420. This hydrolysis required the presence of thiols (6 mM) and much ionic strength (1 M KCl) and was reversibly inhibited by oxygen. The reaction proceeded optimally at pH 8.2 and was Mn dependent. Conditions for F390 hydrolysis in cell extracts are in many respects opposite to those previously described for F390 synthesis.

Full text

PDF
2288

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Eirich L. D., Vogels G. D., Wolfe R. S. Distribution of coenzyme F420 and properties of its hydrolytic fragments. J Bacteriol. 1979 Oct;140(1):20–27. doi: 10.1128/jb.140.1.20-27.1979. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Eirich L. D., Vogels G. D., Wolfe R. S. Proposed structure for coenzyme F420 from Methanobacterium. Biochemistry. 1978 Oct 31;17(22):4583–4593. doi: 10.1021/bi00615a002. [DOI] [PubMed] [Google Scholar]
  3. Ellermann J., Hedderich R., Böcher R., Thauer R. K. The final step in methane formation. Investigations with highly purified methyl-CoM reductase (component C) from Methanobacterium thermoautotrophicum (strain Marburg). Eur J Biochem. 1988 Mar 15;172(3):669–677. doi: 10.1111/j.1432-1033.1988.tb13941.x. [DOI] [PubMed] [Google Scholar]
  4. Fox J. A., Livingston D. J., Orme-Johnson W. H., Walsh C. T. 8-Hydroxy-5-deazaflavin-reducing hydrogenase from Methanobacterium thermoautotrophicum: 1. Purification and characterization. Biochemistry. 1987 Jul 14;26(14):4219–4227. doi: 10.1021/bi00388a007. [DOI] [PubMed] [Google Scholar]
  5. Gloss L. M., Hausinger R. P. Methanogen factor 390 formation: species distribution, reversibility and effects of non-oxidative cellular stresses. Biofactors. 1988 Oct;1(3):237–240. [PubMed] [Google Scholar]
  6. Hausinger R. P., Orme-Johnson W. H., Walsh C. Factor 390 chromophores: phosphodiester between AMP or GMP and methanogen factor 420. Biochemistry. 1985 Mar 26;24(7):1629–1633. doi: 10.1021/bi00328a010. [DOI] [PubMed] [Google Scholar]
  7. Jacobson F. S., Daniels L., Fox J. A., Walsh C. T., Orme-Johnson W. H. Purification and properties of an 8-hydroxy-5-deazaflavin-reducing hydrogenase from Methanobacterium thermoautotrophicum. J Biol Chem. 1982 Apr 10;257(7):3385–3388. [PubMed] [Google Scholar]
  8. Kiener A., Orme-Johnson W. H., Walsh C. T. Reversible conversion of coenzyme F420 to the 8-OH-AMP and 8-OH-GMP esters, F390-A and F390-G, on oxygen exposure and reestablishment of anaerobiosis in Methanobacterium thermoautotrophicum. Arch Microbiol. 1988;150(3):249–253. doi: 10.1007/BF00407788. [DOI] [PubMed] [Google Scholar]
  9. Rouvière P. E., Wolfe R. S. Novel biochemistry of methanogenesis. J Biol Chem. 1988 Jun 15;263(17):7913–7916. [PubMed] [Google Scholar]
  10. Schönheit P., Moll J., Thauer R. K. Nickel, cobalt, and molybdenum requirement for growth of Methanobacterium thermoautotrophicum. Arch Microbiol. 1979 Oct;123(1):105–107. doi: 10.1007/BF00403508. [DOI] [PubMed] [Google Scholar]
  11. Sedmak J. J., Grossberg S. E. A rapid, sensitive, and versatile assay for protein using Coomassie brilliant blue G250. Anal Biochem. 1977 May 1;79(1-2):544–552. doi: 10.1016/0003-2697(77)90428-6. [DOI] [PubMed] [Google Scholar]
  12. Van Beelen P., Geerts W. J., Pol A., Vogels G. D. Quantification of coenzymes and related compounds from methanogenic bacteria by high-performance liquid chromatography. Anal Biochem. 1983 Jun;131(2):285–290. doi: 10.1016/0003-2697(83)90171-9. [DOI] [PubMed] [Google Scholar]

Articles from Journal of Bacteriology are provided here courtesy of American Society for Microbiology (ASM)

RESOURCES