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. 1991 Apr;173(8):2707–2709. doi: 10.1128/jb.173.8.2707-2709.1991

Nomenclature relating to restriction of modified DNA in Escherichia coli.

E A Raleigh 1, J Benner 1, F Bloom 1, H D Braymer 1, E DeCruz 1, K Dharmalingam 1, J Heitman 1, M Noyer Weidner 1, A Piekarowicz 1, P L Kretz 1, et al.
PMCID: PMC207841  PMID: 2013582

Abstract

At least three restriction systems that attack DNA containing naturally modified bases have been found in common Escherichia coli K-12 strains. These systems are McrA, McrBC, and Mrr. A brief summary of the genetic and phenotypic properties so far observed in laboratory strains is set forth, together with a proposed nomenclature for describing these properties.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Blumenthal R. M., Gregory S. A., Cooperider J. S. Cloning of a restriction-modification system from Proteus vulgaris and its use in analyzing a methylase-sensitive phenotype in Escherichia coli. J Bacteriol. 1985 Nov;164(2):501–509. doi: 10.1128/jb.164.2.501-509.1985. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Dharmalingam K., Goldberg E. B. Phage-coded protein prevents restriction of unmodified progeny T4 DNA. Nature. 1976 Apr 1;260(5550):454–456. doi: 10.1038/260454a0. [DOI] [PubMed] [Google Scholar]
  3. Dharmalingam K., Revel H. R., Goldberg E. B. Physical mapping and cloning of bacteriophage T4 anti-restriction endonuclease gene. J Bacteriol. 1982 Feb;149(2):694–699. doi: 10.1128/jb.149.2.694-699.1982. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Dila D., Sutherland E., Moran L., Slatko B., Raleigh E. A. Genetic and sequence organization of the mcrBC locus of Escherichia coli K-12. J Bacteriol. 1990 Sep;172(9):4888–4900. doi: 10.1128/jb.172.9.4888-4900.1990. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Grant S. G., Jessee J., Bloom F. R., Hanahan D. Differential plasmid rescue from transgenic mouse DNAs into Escherichia coli methylation-restriction mutants. Proc Natl Acad Sci U S A. 1990 Jun;87(12):4645–4649. doi: 10.1073/pnas.87.12.4645. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Heitman J., Model P. Site-specific methylases induce the SOS DNA repair response in Escherichia coli. J Bacteriol. 1987 Jul;169(7):3243–3250. doi: 10.1128/jb.169.7.3243-3250.1987. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Kohler S. W., Provost G. S., Kretz P. L., Dycaico M. J., Sorge J. A., Short J. M. Development of a short-term, in vivo mutagenesis assay: the effects of methylation on the recovery of a lambda phage shuttle vector from transgenic mice. Nucleic Acids Res. 1990 May 25;18(10):3007–3013. doi: 10.1093/nar/18.10.3007. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Kretz P. L., Reid C. H., Greener A., Short J. M. Effect of lambda packaging extract mcr restriction activity on DNA cloning. Nucleic Acids Res. 1989 Jul 11;17(13):5409–5409. doi: 10.1093/nar/17.13.5409. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Noyer-Weidner M., Diaz R., Reiners L. Cytosine-specific DNA modification interferes with plasmid establishment in Escherichia coli K12: involvement of rglB. Mol Gen Genet. 1986 Dec;205(3):469–475. doi: 10.1007/BF00338084. [DOI] [PubMed] [Google Scholar]
  10. Piekarowicz A., Yuan R., Stein D. C. Isolation of temperature-sensitive McrA and McrB mutations and complementation analysis of the McrBC region of Escherichia coli K-12. J Bacteriol. 1991 Jan;173(1):150–155. doi: 10.1128/jb.173.1.150-155.1991. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Raleigh E. A., Murray N. E., Revel H., Blumenthal R. M., Westaway D., Reith A. D., Rigby P. W., Elhai J., Hanahan D. McrA and McrB restriction phenotypes of some E. coli strains and implications for gene cloning. Nucleic Acids Res. 1988 Feb 25;16(4):1563–1575. doi: 10.1093/nar/16.4.1563. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Raleigh E. A., Trimarchi R., Revel H. Genetic and physical mapping of the mcrA (rglA) and mcrB (rglB) loci of Escherichia coli K-12. Genetics. 1989 Jun;122(2):279–296. doi: 10.1093/genetics/122.2.279. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Raleigh E. A., Wilson G. Escherichia coli K-12 restricts DNA containing 5-methylcytosine. Proc Natl Acad Sci U S A. 1986 Dec;83(23):9070–9074. doi: 10.1073/pnas.83.23.9070. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Ravi R. S., Sozhamannan S., Dharmalingam K. Transposon mutagenesis and genetic mapping of the rglA and rglB loci of Escherichia coli. Mol Gen Genet. 1985;198(3):390–392. doi: 10.1007/BF00332928. [DOI] [PubMed] [Google Scholar]
  15. Revel H. R. Restriction of nonglucosylated T-even bacteriophage: properties of permissive mutants of Escherichia coli B and K12. Virology. 1967 Apr;31(4):688–701. doi: 10.1016/0042-6822(67)90197-3. [DOI] [PubMed] [Google Scholar]
  16. Ross T. K., Achberger E. C., Braymer H. D. Identification of a second polypeptide required for McrB restriction of 5-methylcytosine-containing DNA in Escherichia coli K12. Mol Gen Genet. 1989 Apr;216(2-3):402–407. doi: 10.1007/BF00334382. [DOI] [PubMed] [Google Scholar]
  17. Ross T. K., Achberger E. C., Braymer H. D. Nucleotide sequence of the McrB region of Escherichia coli K-12 and evidence for two independent translational initiation sites at the mcrB locus. J Bacteriol. 1989 Apr;171(4):1974–1981. doi: 10.1128/jb.171.4.1974-1981.1989. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. Ross T. K., Braymer H. D. Localization of a genetic region involved in McrB restriction by Escherichia coli K-12. J Bacteriol. 1987 Apr;169(4):1757–1759. doi: 10.1128/jb.169.4.1757-1759.1987. [DOI] [PMC free article] [PubMed] [Google Scholar]
  19. Sozhamannan S., Dharmalingam K. Molecular cloning and identification of protein products of rglB locus of Escherichia coli. Gene. 1988 Dec 25;74(1):51–52. doi: 10.1016/0378-1119(88)90249-1. [DOI] [PubMed] [Google Scholar]
  20. Whittaker P. A., Campbell A. J., Southern E. M., Murray N. E. Enhanced recovery and restriction mapping of DNA fragments cloned in a new lambda vector. Nucleic Acids Res. 1988 Jul 25;16(14B):6725–6736. doi: 10.1093/nar/16.14.6725. [DOI] [PMC free article] [PubMed] [Google Scholar]
  21. Woodcock D. M., Crowther P. J., Diver W. P., Graham M., Bateman C., Baker D. J., Smith S. S. RglB facilitated cloning of highly methylated eukaryotic DNA: the human L1 transposon, plant DNA, and DNA methylated in vitro with human DNA methyltransferase. Nucleic Acids Res. 1988 May 25;16(10):4465–4482. doi: 10.1093/nar/16.10.4465. [DOI] [PMC free article] [PubMed] [Google Scholar]
  22. Woodcock D. M., Crowther P. J., Doherty J., Jefferson S., DeCruz E., Noyer-Weidner M., Smith S. S., Michael M. Z., Graham M. W. Quantitative evaluation of Escherichia coli host strains for tolerance to cytosine methylation in plasmid and phage recombinants. Nucleic Acids Res. 1989 May 11;17(9):3469–3478. doi: 10.1093/nar/17.9.3469. [DOI] [PMC free article] [PubMed] [Google Scholar]
  23. van de Putte P., Plasterk R., Kuijpers A. A Mu gin complementing function and an invertible DNA region in Escherichia coli K-12 are situated on the genetic element e14. J Bacteriol. 1984 May;158(2):517–522. doi: 10.1128/jb.158.2.517-522.1984. [DOI] [PMC free article] [PubMed] [Google Scholar]

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