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. 1991 Jul;173(14):4540–4543. doi: 10.1128/jb.173.14.4540-4543.1991

Use of an Escherichia coli beta-glucuronidase gene as a reporter gene for investigation of Bacteroides promoters.

M J Feldhaus 1, V Hwa 1, Q Cheng 1, A A Salyers 1
PMCID: PMC208121  PMID: 2066346

Abstract

We have constructed transcriptional fusion vectors for use in Bacteroides spp., a genus of gram-negative obligate anaerobes found in high numbers in the human colon. The reporter group in these vectors is a promoterless beta-glucuronidase gene from Escherichia coli (uidA). Two of the vectors (pMJF-2 and pMJF-3) replicate in Bacteroides spp. The third, pCQW-1, does not replicate in Bacteroides spp. and can be used to introduce E. coli beta-glucuroindase fusions into the Bacteroides chromosome.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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