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. 1990 Feb;172(2):1145–1147. doi: 10.1128/jb.172.2.1145-1147.1990

Detection of lipopolysaccharides by ethidium bromide staining after sodium dodecyl sulfate-polyacrylamide gel electrophoresis.

N Kido 1, M Ohta 1, N Kato 1
PMCID: PMC208550  PMID: 1688839

Abstract

A rapid and easy method for staining lipopolysaccharides with ethidium bromide is described. Lipopolysaccharides could be visualized by ethidium bromide with almost the same sensitivity as found with the silver-staining method in less than 30 min. The ethidium bromide-staining method was particularly suitable for staining lipopolysaccharides possessing acidic O-specific polysaccharides, which were poorly visualized by silver staining.

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Selected References

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  1. Dmitriev B. A., Backinowsky L. V., Lvov V. L., Kochetkov N. K., Hofman I. L. Somatic antigen of Shigella dysenteriae type 3. Structural features of specific polysaccharide chain. Eur J Biochem. 1975 Jan 15;50(3):539–547. doi: 10.1111/j.1432-1033.1975.tb09894.x. [DOI] [PubMed] [Google Scholar]
  2. Dmitriev B. A., Knirel Y. A., Sheremet O. K., Shashkov A. A., Kochetkov N. K., Hofman I. L. Somatic antigens of Shigella. The structure of the specific polysaccharide of Shigella newcastle (Sh. flexneri type 6) lipopolysaccharide. Eur J Biochem. 1979 Jul;98(1):309–316. doi: 10.1111/j.1432-1033.1979.tb13190.x. [DOI] [PubMed] [Google Scholar]
  3. Goldman R. C., Leive L. Heterogeneity of antigenic-side-chain length in lipopolysaccharide from Escherichia coli 0111 and Salmonella typhimurium LT2. Eur J Biochem. 1980;107(1):145–153. doi: 10.1111/j.1432-1033.1980.tb04635.x. [DOI] [PubMed] [Google Scholar]
  4. Hitchcock P. J., Brown T. M. Morphological heterogeneity among Salmonella lipopolysaccharide chemotypes in silver-stained polyacrylamide gels. J Bacteriol. 1983 Apr;154(1):269–277. doi: 10.1128/jb.154.1.269-277.1983. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Jann B., Reske K., Jann K. Heterogeneity of lipopolysaccharides. Analysis of polysaccharide chain lengths by sodium dodecylsulfate-polyacrylamide gel electrophoresis. Eur J Biochem. 1975 Dec 1;60(1):239–246. doi: 10.1111/j.1432-1033.1975.tb20996.x. [DOI] [PubMed] [Google Scholar]
  6. Kido N., Ohta M., Iida K., Hasegawa T., Ito H., Arakawa Y., Komatsu T., Kato N. Partial deletion of the cloned rfb gene of Escherichia coli O9 results in synthesis of a new O-antigenic lipopolysaccharide. J Bacteriol. 1989 Jul;171(7):3629–3633. doi: 10.1128/jb.171.7.3629-3633.1989. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Kontrohr T. The identification of 2-amino-2-deoxy-L-altruronic acid as a constituent of Shigella sonnei phase I lipopolysaccharide. Carbohydr Res. 1977 Oct;58(2):498–500. doi: 10.1016/s0008-6215(00)84379-7. [DOI] [PubMed] [Google Scholar]
  8. Munford R. S., Hall C. L., Rick P. D. Size heterogeneity of Salmonella typhimurium lipopolysaccharides in outer membranes and culture supernatant membrane fragments. J Bacteriol. 1980 Nov;144(2):630–640. doi: 10.1128/jb.144.2.630-640.1980. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Palva E. T., Mäkelä P. H. Lipopolysaccharide heterogeneity in Salmonella typhimurium analyzed by sodium dodecyl sulfate polyacrylamide gel electrophoresis. Eur J Biochem. 1980;107(1):137–143. doi: 10.1111/j.1432-1033.1980.tb04634.x. [DOI] [PubMed] [Google Scholar]
  10. Reske K., Jann K. The O8 antigen of Escherichia coli. Structure of the polysaccharide chain. Eur J Biochem. 1972 Dec 4;31(2):320–328. doi: 10.1111/j.1432-1033.1972.tb02536.x. [DOI] [PubMed] [Google Scholar]
  11. Russell R. R., Johnson K. G. SDS-polyacrylamide gel electrophoresis of lipopolysaccharides. Can J Microbiol. 1975 Dec;21(12):2013–2018. doi: 10.1139/m75-289. [DOI] [PubMed] [Google Scholar]
  12. Tsai C. M., Frasch C. E. A sensitive silver stain for detecting lipopolysaccharides in polyacrylamide gels. Anal Biochem. 1982 Jan 1;119(1):115–119. doi: 10.1016/0003-2697(82)90673-x. [DOI] [PubMed] [Google Scholar]

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