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. 1990 May;172(5):2230–2235. doi: 10.1128/jb.172.5.2230-2235.1990

Transfer region of IncI1 plasmid R64 and role of shufflon in R64 transfer.

T Komano 1, N Funayama 1, S R Kim 1, T Nisioka 1
PMCID: PMC208852  PMID: 1970558

Abstract

To locate the transfer region of the 122-kiloase plasmid R64drd-11 belonging to incompatibility group I1, a series of deletion derivatives was constructed by in vitro recombinant DNA techniques followed by double homologous recombination in vivo. A plasmid designated pKK609 and bearing a 56.7-kilobase R64 sequence was the smallest transferable plasmid. A plasmid designated pKK610 and no longer possessing the 44-base-pair sequence of the R64 transfer system is located at one end. The other end of the R64 transfer region comprises a DNA segment of about 19 kilobases responsible for pilus formation. Shufflon, DNA with a novel rearrangement in R64, was found to be involved in pilus formation.

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Selected References

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